Acoustic wave biosensors: physical models and biological applications of quartz crystal microbalance

Ferreira, Guilherme N. M.; Dasilva, A. M.; Tomé, Brigitte

doi:10.1016/j.tibtech.2009.09.003

Cited by 215 publications

(152 citation statements)

References 59 publications

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“…Unfortunately, the mere use of PEG as carrier material for antigens is not sufficient in order to significantly reduce non-specific protein interaction, although PEG hydrogels are widely believed to be protein-repellent materials. Our finding is in line with recent studies showing that the protein-repellent properties attributed to PEG are in fact due to a protein corona that forms around PEG in blood [3]. This means that PEG does interact non-specifically with serum albumin.…”

Section: Characterization Of Soil Release Polymers By Scp Adhesion Assupporting

confidence: 92%

“…Controlling adhesive interaction under water is also very important in technology, e.g., mussel adhesion on surfaces of ships as well as for cleaning processes or controlling the haptic sensation of surfaces [1,2]. On the molecular level, adhesion phenomena are typically indirectly studied using methods like quartz crystal microbalance, surface plasmon resonance or fluorescence microscopy [3,4]. These methods simply relate adhesion to the adsorbed amount of the molecules of interest.…”

Section: Introductionmentioning

confidence: 99%

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Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers

Strzelczyk

Wang

Lindhorst

et al. 2017

Gels

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Adhesive processes in aqueous media play a crucial role in nature and are important for many technological processes. However, direct quantification of adhesion still requires expensive instrumentation while their sample throughput is rather small. Here we present a fast, and easily applicable method on quantifying adhesion energy in water based on interferometric measurement of polymer microgel contact areas with functionalized glass slides and evaluation via the Johnson-Kendall-Roberts (JKR) model. The advantage of the method is that the microgel matrix can be easily adapted to reconstruct various biological or technological adhesion processes. Here we study the suitability of the new adhesion method with two relevant examples: (1) antibody detection and (2) soil release polymers. The measurement of adhesion energy provides direct insights on the presence of antibodies showing that the method can be generally used for biomolecule detection. As a relevant example of adhesion in technology, the antiadhesive properties of soil release polymers used in today's laundry products are investigated. Here the measurement of adhesion energy provides direct insights into the relation between polymer composition and soil release activity. Overall, the work shows that polymer hydrogel particles can be used as versatile adhesion sensors to investigate a broad range of adhesion processes in aqueous media.

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Section: Characterization Of Soil Release Polymers By Scp Adhesion Assupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers

Strzelczyk

Wang

Lindhorst

et al. 2017

Gels

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“…3 Bonroy et al showed that the equation is valid in an electrodeposited porous gold substrate. 14 We evaluated IgG binding to a Protein A-immobilized sensor chip (Fig.…”

Section: Qcm Sensingmentioning

confidence: 99%

“…1 In general, ligand molecules are chemically immobilized on the solid surface of a sensor chip, to which analyte molecules in solution specifically bind. The resultant change of the surface characteristics are detected by such phenomena as electrochemical reactions, 2 quartz crystal microbalance (QCM), 3 surface plasmon resonance (SPR) 4 and surface acoustic wave (SAW). 5 An enhancement of the sensitivity has attracted strong interest in terms of the detection of a sample of low concentration and small molecules.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Electrodeposited Gold Nanostructures for Applications in QCM Sensing

et al. 2012

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The electrodeposition of gold nanostructures increases the surface area of a biosensor, which brings an enhancement of the sensitivity by increasing the amount of analyte binding to the surface. To evaluate the relationship among the surface structure, the area and the analyte binding, we quantitatively analyzed them for quartz crystal microbalance (QCM) sensing by scanning electron microscopy and cyclic voltammetry measurements. The results indicate a several-times increase of analyte bindings, and also the limitation of the sensing performance.

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“…Equation 44 shows the decay characteristic length as proportional to the ratio viscosity to density of the liquid and as inversely proportional to the angular frequency (ω) [58]. For 10MHz AT cut quartz crystal this length is: δ=178nm.…”

Section: Qcr In Contact With Fluidsmentioning

confidence: 99%

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

Ahumada¹,

Armando²

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IVResumen de la tesis de Luis Armando Carvajal Ahumada, presentada como requisito parcial para la obtención del grado de DOCTOR en INGENIERÍA BIOMÉDICA. Madrid, 03 de Abril de 2017 DISEÑO Y EVALUACIÓN DE UN SENSOR BASADO EN RESONADORES DE CRISTAL DE CUARZO (QCR) PARA CARACTERIZAR MUESTRAS BIOLÓGICAS RELACIONADAS CON LA DIAGNOSIS DE ENFERMEDADES ARTRÍTICASResumen:Los resonadores de cristal de cuarzo son dispositivos ampliamente utilizados como biosensores debido a su alta sensibilidad ante fenómenos físico-químicos que ocurren en su superficie, a la reproducibilidad de las mediciones y por ser transductores fáciles de utilizar. Sauerbrey[1] fue el primero en demostrar que el resonador cristal de cuarzo puede ser utilizado como una microbalanza útil para caracterizar pequeñas deposiciones de masa en el electrodo del cristal. Sauerbrey demostró que, si se deposita una delgada capa de material rígido sobre el cristal de cuarzo, tanto el cristal como el material depositado vibrarán a la misma frecuencia y se generará un cambio en la frecuencia de resonancia inicial del cristal. Específicamente, la frecuencia de resonancia disminuye al aumentar la cantidad de masa depositada.El cristal de cuarzo también puede operar en medio fluidos. En este caso además del desplazamiento en frecuencia del cristal, se genera una pérdida significativa en el factor de calidad del resonador. Para fluidos Newtonianos (viscosidad constante), Kanazawa y Gordon[2] demostraron que el desplazamiento en frecuencia que sufre el resonador así como el cambio en el ancho de la curva de admitancia, depende de la densidad y la viscosidad del líquido. Este fenómeno permite utilizar los resonadores de cristal de cuarzo como pequeños viscosímetros siempre que se conozca la densidad del fluido a analizar.En el caso de deposición de fluidos viscoelásticos, la medición del cambio en la frecuencia de resonancia del cristal no es suficiente para obtener el valor de viscosidad del fluido. Por tanto, se requiere de la medición de una segunda variable (mitad del ancho de media banda -Γ) para obtener el valor correcto de la viscosidad del fluido depositado.[3] Para fluidos pseudoplásticos (viscosidad aparente dependiente de la velocidad de cizallamiento aplicada), se requiere de un modelo matemático que explique el comportamiento de la viscosidad del fluido a distintas velocidades de cizallamiento. En particular para el análisis de muestras con este comportamiento, se ha elegido el modelo Rouse ya que explica con suficiente detalle el comportamiento pseudoplástico y resulta simple de aplicar a las medidas experimentales.En este trabajo se propone el diseño de un biosensor basado en un resonador de cristal de cuarzo (QCR) para la caracterización de muestras biológicas que emulan el comportamiento del líquido sinovial.El líquido sinovial es una mezcla de plasma dializado y ácido hialurónico con comportamiento pseudoplástico. Su principal función es la de actuar como lubricante en las articulaciones V manteniendo al mínimo la fricción entre los huesos, especialment...

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Acoustic wave biosensors: physical models and biological applications of quartz crystal microbalance

Cited by 215 publications

References 59 publications

Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers

Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers

Evaluation of Electrodeposited Gold Nanostructures for Applications in QCM Sensing

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

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