Acridine orange association equilibrium in aqueous solution

WITHDRAWN: Preconcentration and indirect quantification of trace nitrite, nitrate and total nitrite in selected beverage and milk samples using ion-pairing cloud-point extraction with acridine orange

“…Also, below (4-5) Â 10 À5 mol L À1 , an 251 isobestic point is observed at 471.5 nm. However, at higher 252 concentrations, it has been observed that a peak at 465 nm is 253 changed to 450 nm with a blue shift of 15 nm, and the isobestic 254 point disappears (Costantino et al, 1984). This may be due to 255 charge transfer based on acid-base interactions between I 3 À and 256 AOH + or (AOH) 2 2+ as well as dimerization of dye, as it is also 257 observed in the existing study.…”

Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy

“…Here, we present the rationale for using R/GFIR instead of red fluorescence to assess the metachromatic shift induced by molecular stacking of Acridine Orange concentrated due to its protonation in AVOs (Costantino et al, 1984). Control cells have a level of R/GFIR due to the red fluorescence in lysosomes and basal autophagy, but autophagy induction augments AVO volume due to the increase in autolysosomes, and the measurement of Acridine Orange R/GFIR can detect this increase.…”

A Quantitative Model for Using Acridine Orange as a Transmembrane pH Gradient Probe

“…For AO, this process was investigated first by Zanker (25). The latest data obtained by absorbance spectroscopy (30) give the following thermodynamic parameters for AO dimerization in aqueous solutions: ⌬H 12 ϭ Ϫ30.8 kJ/ mol and ⌬S 12 ϭ Ϫ25.7 J/mol/K. The solid line in Fig.…”