Acrylamide Gel as a Supporting Medium for Zone Electrophoresis

Raymond, Samuel; Weintraub, Lewis

doi:10.1126/science.130.3377.711.a

Cited by 43 publications

(3 citation statements)

References 2 publications

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“…Tadamasa KANAOKA 1 , Tatsuro ENDO 2 , Hideaki HISAMOTO 2 and Kenji SUEYOSHI ＊2,3 ＊ E-mail : sueyoshi@omu.ac.jp 1 Department of Material Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-ku, Sakai-shi, Osaka 599-8531 2 Department of Material Chemistry, Graduate School of Engineering, Osaka Metropolitan University, 1-1, Gakuen-cho, Naka-ku, Sakai-shi, Osaka 599-8531 3 CREST, Japan Science and Technology Agency, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076 (Received June 5, 2023; Accepted June 20, 2023)…”

Section: Development Of a Cartridge Device Integrated With Multiple H...mentioning

confidence: 99%

二次元デジタル電気泳動分離のための異種機能性ハイドロゲル集積カートリッジデバイスの開発

KANAOKA,

ENDO,

HISAMOTO

et al. 2023

Bunseki kagaku

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Section: Development Of a Cartridge Device Integrated With Multiple H...mentioning

confidence: 99%

二次元デジタル電気泳動分離のための異種機能性ハイドロゲル集積カートリッジデバイスの開発

KANAOKA,

ENDO,

HISAMOTO

et al. 2023

Bunseki kagaku

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“…Hydrogels consist of a network of polymer chains that are linked together by a crosslinker like methylenebisacrylamide in acrylamide gels. 18 The ratio between the monomers and crosslinkers defines the mesh size of the polymerized network. 19 Due to the hydrophilic character of the polymer chains, such networks can incorporate large amounts of aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel-driven paper-based microfluidics

Niedl

Beta

2015

Lab Chip

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Paper-based microfluidics provide an inexpensive, easy to use technology for point-of-care diagnostics in developing countries. Here, we combine paper-based microfluidic devices with responsive hydrogels to add an entire new class of functions to these versatile low-cost fluidic systems. The hydrogels serve as fluid reservoirs. In response to an external stimulus, e.g. an increase in temperature, the hydrogels collapse and release fluid into the structured paper substrate. In this way, chemicals that are either stored on the paper substrate or inside the hydrogel pads can be dissolved, premixed, and brought to reaction to fulfill specific analytic tasks. We demonstrate that multi-step sequences of chemical reactions can be implemented in a paper-based system and operated without the need for external precision pumps. We exemplify this technology by integrating an antibody-based E. coli test on a small and easy to use paper device.

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“…Traditional polyacrylamide/agarose gel electrophoresis (GE) has been applied as one of the most general tools in biologic study, , protein identification, , nucleic acid sequencing, , genetic engineering drugs, antibody for cancer treatment, , as well as clinic diagnosis , since its introduction by Remond and Weintraub in 1959. − However, traditional slab GE suffered from labor and time consumption due to complex handling of protein or nucleic acid band fixing, staining, destaining, and scanning after a GE run.…”

mentioning

confidence: 99%

Gel Electrophoresis Chip Using Joule Heat Self-Dissipation, Short Run Time, and Online Dynamic Imaging

et al. 2021

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Gel electrophoresis (GE) is one of the most general tools in biomedicine. However, it suffers from low resolution, and its mechanism has not been fully revealed yet. Herein, we presented the dispersion model of w 2 (t) ∝ Tt, showing the band dispersion (w) via temperature (T) and running time (t) control. Second, we designed an efficient GE chip via the time control and rapid Joule heat self-dissipation by thermal conductive plastic (TCP) and electrode buffer. Third, we conducted the simulations on TCP and polymethylmethacrylate (PMMA) chips, unveiling that (i) the temperature of TCP was lower than the PMMA one, (ii) the temperature uniformity of TCP was better than the PMMA one, and (iii) the resolution of TCP was superior to the PMMA one. Fourth, we designed both TCP and PMMA chips for experimentally validating the dispersion model, TCP chip, and simulations. Finally, we applied the TCP chip to thalassemia and model urine protein assays. The TCP chip has merits of high resolution, rapid run of 6–10 min, and low cost. This work paves the way for greatly improving electrophoretic techniques in gel, chip, and capillary via temperature and time control for biologic study, biopharma quality control, clinical diagnosis, and so on.

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Acrylamide Gel as a Supporting Medium for Zone Electrophoresis

Abstract: Acrylamide polymerized in buffer solutions forms a stable, flexible, transparent gel which is useful in zone electrophoresis.

Cited by 43 publications

References 2 publications

二次元デジタル電気泳動分離のための異種機能性ハイドロゲル集積カートリッジデバイスの開発

二次元デジタル電気泳動分離のための異種機能性ハイドロゲル集積カートリッジデバイスの開発

Hydrogel-driven paper-based microfluidics

Gel Electrophoresis Chip Using Joule Heat Self-Dissipation, Short Run Time, and Online Dynamic Imaging

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