Acrylamide impairs ovarian function by promoting apoptosis and affecting reproductive hormone release, steroidogenesis and autophagy-related genes: An in vivo study

Aldawood, Nouf; Alrezaki, Abdulkarem; Alanazi, Shamsa; Amor, Nabil; Alwasel, Saleh; Sirotkin, Alexander V.; Harrath, Abdel Halim

doi:10.1016/j.ecoenv.2020.110595

Cited by 42 publications

(27 citation statements)

References 79 publications

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“…especially the ovary, which is considered the most important organ in a woman's reproductive and endocrine processes [35]. Many reproductive health issues have been linked to EDC exposure, including miscarriage, premature ovarian failure, and elevated sex steroid hormone levels.…”

Section: Discussionmentioning

confidence: 99%

Fetal programming: in utero exposure to acrylamide leads to intergenerational disrupted ovarian function and accelerated ovarian aging

Aldawood

Jalouli

Alrezaki

et al. 2022

Aging

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In this study we investigated the effects of multigenerational exposures to acrylamide (ACR) on ovarian function. Fifty-day-old Wistar albino female rats were divided into the control and ACR-treated groups (2.5, 10, and 20 mg/kg/day) from day 6 of pregnancy until delivery. The obtained females of the first (AF1) and second generation (AF2) were euthanized at 4 weeks of age, and plasma and ovary samples were collected. We found that in utero multigenerational exposure to ACR reduced fertility and ovarian function in AF1 through inducing histopathological changes as evidenced by the appearance of cysts and degenerating follicles, oocyte vacuolization, and pyknosis in granulosa cells. TMR red positive cells confirmed by TUNEL assay were mostly detected in the stroma of the treated groups. Estradiol and IGF-1 concentrations significantly decreased as a result of decreased CYP19 gene and its protein expression. However, ACR exposure in AF2 led to early ovarian aging as evidenced by high estradiol and progesterone levels among all treated groups compared to control group, corresponding to the upregulation of the CYP19 gene and protein expression. The apoptotic cells of the stroma were greatly detected compared to that in the control group, whereas no significant difference was reported in ESR1 and ESR2 gene expression. This study confirms the developmental adverse effects of ACR on ovarian function and fertility in at least two consecutive generations. It emphasizes the need for more effective strategies during pregnancy, such as eating healthy foods and avoiding consumption of ACR-rich products, including fried foods and coffee.

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Section: Discussionmentioning

confidence: 99%

Fetal programming: in utero exposure to acrylamide leads to intergenerational disrupted ovarian function and accelerated ovarian aging

Aldawood

Jalouli

Alrezaki

et al. 2022

Aging

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“…Immunofluorescence analysis was performed according to the methods used in our previous studies [ 64 , 65 ]. Briefly, 3-µm-thick sections of ovarian tissues were dewaxed, hydrated with gradient ethanol and washed twice with distilled water and three times with 1x PBS.…”

Section: Methodsmentioning

confidence: 99%

Allethrin Promotes Apoptosis and Autophagy Associated with the Oxidative Stress-Related PI3K/AKT/mTOR Signaling Pathway in Developing Rat Ovaries

Jalouli

Mofti

Elnakady

et al. 2022

IJMS

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The increased concern regarding the reduction in female fertility and the impressive numbers of women undergoing fertility treatment support the existence of environmental factors beyond inappropriate programming of developing ovaries. Among these factors are pyrethroids, which are currently some of the most commonly used pesticides worldwide. The present study was performed to investigate the developmental effects of the pyrethroid-based insecticide allethrin on ovarian function in rat offspring in adulthood. We mainly focused on the roles of oxidative stress, apoptosis, autophagy and the related pathways in ovarian injury. Thirty-day-old Wistar albino female rats were intragastrically administered 0 (control), 34.2 or 68.5 mg/kg body weight allethrin after breeding from Day 6 of pregnancy until delivery. We found that allethrin-induced ovarian histopathological damage was accompanied by elevations in oxidative stress and apoptosis. Interestingly, the number of autophagosomes in allethrin-treated ovaries was higher, and this increase was correlated with the upregulated expression of genes and proteins related to the autophagic marker LC-3. Furthermore, allethrin downregulated the expression of PI3K, AKT and mTOR in allethrin-treated ovaries compared with control ovaries. Taken together, the findings of this study suggest that exposure to the pyrethroid-based insecticide allethrin adversely affects both the follicle structure and function in rat offspring during adulthood. Specifically, allethrin can induce excessive oxidative stress and defective autophagy-related apoptosis, probably through inactivation of the PI3K/AKT/mTOR signaling pathway, and these effects may contribute to ovarian dysfunction and impaired fertility in female offspring.

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“…José García Martín, who received the FF UFK commemorative medal from the hand of the dean (Zdenka Gadušová in 2010), as a reward for his decisive contribution to international Kierkegaardian research; likewise, the visit of the Vice-Rector for International Relations of the UFK, Martina Pavliková, to the University of Granada, from which a collaboration between both universities was formally established, should be noted. Biologist, Prof. Alexander Sirotkin, has a long history of working with scientists from Saudi Arabia [39,40]. Cooperation with Czech scholars and scientists is also very fruitful: Prof. František Petrovič has been developing cooperation in the field of environmental sciences [41,42]; they are experts in Geography and cooperate in the field of services [43]; as well as Assoc.…”

Section: Selected Publication Outputs Of Ukf Staff With Foreign Researchersmentioning

confidence: 99%

How to Keep University Active during COVID-19 Pandemic: Experience from Slovakia

et al. 2021

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The paper outlines the adverse consequences and challenges induced by COVID-19 pandemic for the whole world and for universities in particular. The example of Constantine the Philosopher University in Nitra illustrates the difficulties and challenges caused by the pandemic in relation to the two main activities arising from the university mission-teaching and research. It presents some particular aspects of the university activities adversely affected by COVID-19 and shares the measures to minimize the resulted damages. Furthermore, it demonstrates that, despite complications induced by COVID-19, teaching, research, and international cooperation have been successfully continued.

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Acrylamide impairs ovarian function by promoting apoptosis and affecting reproductive hormone release, steroidogenesis and autophagy-related genes: An in vivo study

Cited by 42 publications

References 79 publications

Fetal programming: in utero exposure to acrylamide leads to intergenerational disrupted ovarian function and accelerated ovarian aging

Fetal programming: in utero exposure to acrylamide leads to intergenerational disrupted ovarian function and accelerated ovarian aging

Allethrin Promotes Apoptosis and Autophagy Associated with the Oxidative Stress-Related PI3K/AKT/mTOR Signaling Pathway in Developing Rat Ovaries

How to Keep University Active during COVID-19 Pandemic: Experience from Slovakia

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