ACRYLAMIDE INDUCED GENOTOXIC EFFECT IN GOLDEN HAMSTER (Mesocricetus auratus) GENOME

Abstract

This work aims at dislosing the capability of acrylamide in causing genotoxic effect in golden hamster genome. To achieve such a purpose, five different doses that ranged from 1 to 20 mg/ kg b. wt. were chosen and tested employing three genotoxic bioassays. These assays are : 1-Estimation of cell proliferation, 2-Analysis of chromosomal abnormalities; and 3-In vivo induction of sister chromatid exchanges.The results obtained showed that all tested doses were found to be toxic and cellular toxicity was observed. Analysis o chromosomal aberrations revealed that acrylamide is a positive clastogenic agent. Sister chromatid exchange assay indicated that acrylamide is a powerful inducer of primary genetic damage.