Actin Motility: Staying on Track Takes a Little More Effort

Ehrenberg, Morton; McGrath, James L.

doi:10.1016/j.cub.2004.10.018

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…3D), consistent with the concept that these track-like structures are working in concert with the MT-based tracks, in coordination with motor proteins (e.g., myosin VIIa) (Fig. 3B, C) to support transport of cell organelles (e.g., residual bodies, phagosomes) and likely developing spermatids during the epithelial cycle, similar to other mammalian cells and/or epithelia 35 (Fig. 4).…”

Section: Role Of Microtubule (Mt)-based Cytoskeletonsupporting

confidence: 70%

“…The eventual winning motor protein that dictates the directional transport of a cargo is determined by the presence of protein kinases, phosphatases and/or Rab GTPases associated with the motor protein, which can modify the activity of a motor protein through changes in the adaptor proteins or other microtubule-associated proteins (MAPs). Also involved is the polarized F-actin microfilaments which also serve as the tracks to coordinately support cellular transport such as serving as a guide for MT growth 31,33,35 (Figs. 3 and 4).…”

Section: Role Of Microtubule (Mt)-based Cytoskeletonmentioning

confidence: 99%

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Transport of germ cells across the seminiferous epithelium during spermatogenesis—the involvement of both actin- and microtubule-based cytoskeletons

et al. 2016

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The transport of germ cells from the base of the seminiferous epithelium toward the luminal edge of the tubule lumen in the adluminal compartment during the epithelial cycle is an essential cellular event to support spermatogenesis. Thus, fully developed elongated spermatids (i.e., spermatozoa) can be released at spermiation in late stage VIII in rodents versus late stage II in humans. Earlier studies to examine the molecular mechanism(s) that support germ cell transport, most notably the transport of preleptotene spermatocytes across the blood-testis barrier (BTB), and the transport of elongating spermatids across the adluminal compartment during spermiogenesis, is focused on the adhesion protein complexes at the cell-cell interface. It is generally accepted that cell junctions at the Sertoli cell-cell interface at the BTB, including the actin-based tight junction (TJ), basal ectoplasmic specialization (basal ES, a testis-specific adherens junction) and gap junction (GJ), as well as the intermediate filament-based desmosome undergo constant remodeling to accommodate the transport of preleptotene spermatocytes across the barrier. On the other hand, similar junction dynamics (i.e., disassembly, reassembly and stabilization/maintenance) take place at the Sertoli-spermatid interface. Emerging evidence has shown that junction dynamics at the Sertoli cell-cell vs. Sertoli-germ cell interface are supported by the 2 intriguingly coordinated cytoskeletons, namely the F-actin-and microtubule (MT)-based cytoskeletons. Herein, we provide a brief summary and critically evaluate the recent findings. We also provide an updated hypothetical concept regarding germ cell transport in the testis utilizing the MT-conferred tracks and the MT-specific motor proteins. Furthermore, this cellular event is also supported by the F-actin-based cytoskeleton.

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Section: Role Of Microtubule (Mt)-based Cytoskeletonsupporting

confidence: 70%

Section: Role Of Microtubule (Mt)-based Cytoskeletonmentioning

confidence: 99%

Transport of germ cells across the seminiferous epithelium during spermatogenesis—the involvement of both actin- and microtubule-based cytoskeletons

et al. 2016

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“…In the cytoplasm, actin and microtubule motors are responsible for moving organelles along filaments (reviewed in Ehrenberg and McGrath, 2004). In the cytoplasm, actin and microtubule motors are responsible for moving organelles along filaments (reviewed in Ehrenberg and McGrath, 2004).…”

Section: Effects Of Inhibiting Actin Polymerization On Nuclear Bodiesmentioning

confidence: 99%

Dynamic Nature of Cleavage Bodies and Their Spatial Relationship to DDX1 Bodies, Cajal Bodies, and Gems

Roy

Katyal

et al. 2006

MBoC

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DDX1 bodies, cleavage bodies, Cajal bodies (CBs), and gems are nuclear suborganelles that contain factors involved in RNA transcription and/or processing. Although all four nuclear bodies can exist as distinct entities, they often colocalize or overlap with each other. To better understand the relationship between these four nuclear bodies, we examined their spatial distribution as a function of the cell cycle. Here, we report that whereas DDX1 bodies, CBs and gems are present throughout interphase, CPSF-100-containing cleavage bodies are predominantly found during S and G2 phases, whereas CstF-64-containing cleavage bodies are primarily observed during S phase. All four nuclear bodies associate with each other during S phase, with cleavage bodies colocalizing with DDX1 bodies, and cleavage bodies/DDX1 bodies residing adjacent to gems and CBs. Although inhibitors of RNA transcription had no effect on DDX1 bodies or cleavage bodies, inhibitors of DNA replication resulted in loss of CstF-64-containing cleavage bodies. A striking effect on nuclear structures was observed with latrunculin B, an inhibitor of actin polymerization, resulting in the formation of needlelike nuclear spicules made up of CstF-64, CPSF-100, RNA, and RNA polymerase II. Our results suggest that cleavage body components are highly dynamic in nature.

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“…3 The term also refers to contests between different species. Ehrenberg and McGrath (2004) refer to the interaction of microtubule motors, Larsson, Beignon and Bhardwaj (2004) and Zhou et al (2004) refer to the interaction between viruses and the dendritic cells or other parts of the immune system as tugs of war. Tibbetts and Reeve (2000) consider the role of the amount of reproductive sharing within a group for the likelihood of within-group conflict among the social wasp Polistes dominulus.…”

Section: Introductionmentioning

confidence: 99%

Equilibrium and Efficiency in the Tug-of-War

Konrad

Kovenock

2005

SSRN Journal

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Actin Motility: Staying on Track Takes a Little More Effort

Abstract: The pigment cells of amphibians and fish have provided excellent models for understanding how actin and microtubule motors move organelles along the complex trailway of filaments in the cytoplasm. Recent work provides more evidence that these motors are engaged in a continuous tug-of-war.

Cited by 4 publications

References 15 publications

Transport of germ cells across the seminiferous epithelium during spermatogenesis—the involvement of both actin- and microtubule-based cytoskeletons

Transport of germ cells across the seminiferous epithelium during spermatogenesis—the involvement of both actin- and microtubule-based cytoskeletons

Dynamic Nature of Cleavage Bodies and Their Spatial Relationship to DDX1 Bodies, Cajal Bodies, and Gems

Equilibrium and Efficiency in the Tug-of-War

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