Actinobacterial endophytes for improved crop performance

Franco, Christopher M. M.; Michelsen, P.; Percy, Nigel; Conn, Vanessa M.; Listiana, Erna; Moll, Simon; Loria, Rosemary; Coombs, Justin T.

doi:10.1071/ap07067

Cited by 69 publications

(47 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on these results, Pseudonocardia was the dominant genus (57%), followed by Nocardiopsis (8.3%), Micromonospora (6.4%), Streptosporangium (3.8%), and other organisms (24.5%) that could not be accurately identified to the genus level. The genera Micromonospora, Streptosporangium, and Nocardiopsis have also been reported frequently from a wide range of host plants, such as maize, lichens, and wheat (21,30,33). Forty-six strains, including representatives of each group, were subjected to 16S rRNA gene sequence analysis.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Diversity, and Antimicrobial Activity of Rare Actinobacteria from Medicinal Plants of Tropical Rain Forests in Xishuangbanna, China

Qin

Chen

et al. 2009

Appl Environ Microbiol

380

263

View full text Add to dashboard Cite

Endophytic actinobacteria are relatively unexplored as potential sources of novel species and novel natural products for medical and commercial exploitation. Xishuangbanna is recognized throughout the world for its diverse flora, especially the rain forest plants, many of which have indigenous pharmaceutical histories. However, little is known about the endophytic actinobacteria of this tropical area. In this work, we studied the diversity of actinobacteria isolated from medicinal plants collected from tropical rain forests in Xishuangbanna. By the use of different selective isolation media and methods, a total of 2,174 actinobacteria were isolated. Forty-six isolates were selected on the basis of their morphologies on different media and were further characterized by 16S rRNA gene sequencing. The results showed an unexpected level of diversity, with 32 different genera. To our knowledge, this is the first report describing the isolation of Saccharopolyspora, Dietzia, Blastococcus, Dactylosporangium, Promicromonospora, Oerskovia, Actinocorallia, and Jiangella species from endophytic environments. At least 19 isolates are considered novel taxa by our current research. In addition, all 46 isolates were tested for antimicrobial activity and were screened for the presence of genes encoding polyketide synthetases and nonribosomal peptide synthetases. The results confirm that the medicinal plants of Xishuangbanna represent an extremely rich reservoir for the isolation of a significant diversity of actinobacteria, including novel species, that are potential sources for the discovery of biologically active compounds.The class Actinobacteria accounts for a high proportion of soil microbial biomass and contains the most economically significant prokaryotes, producing more than half of the bioactive compounds in a literature survey (46), including antibiotics (6), immunosuppressive agents (55), antitumor agents (18), and enzymes (64). Actinobacteria belonging to the genus Streptomyces, in particular, are excellent producers. The emergence of drug resistance in many bacterial pathogens and the current increase in the number of fungal infections has caused a resurgence of interest in finding new reserves of biologically active compounds (63). As the search for novel natural products continues, it becomes apparent that the rate of discovery of new compounds from soil streptomycetes has decreased, whereas the rate of reisolation of known compounds has increased (28). Recently, evidence has accumulated that rare actinomycete species, which are often very difficult to isolate and cultivate, might represent a unique source of novel biologically active compounds (4). On the other hand, new microbial habitats need to be examined in the search for novel bioactive compounds. One biologically important but relatively overlooked niche is the inner tissues of higher plants. Early studies have demonstrated that some actinobacteria can form intimate associations with plants and colonize their inner tissues. Frankia species and Streptomyce...

show abstract

Section: Resultsmentioning

confidence: 99%

Isolation, Diversity, and Antimicrobial Activity of Rare Actinobacteria from Medicinal Plants of Tropical Rain Forests in Xishuangbanna, China

Qin

Chen

et al. 2009

Appl Environ Microbiol

380

263

View full text Add to dashboard Cite

show abstract

“…Reports on such biological control activities by endophytic actinomycetes are relatively few and include the suppression of Fusarium oxysporum Schlecht. Fries (Smith 1957;Cao et al 2005), F. pseudograminearum Aoki and O'Donnell (Franco et al 2007), Verticillium dahliae Kleb. (Krechel et al 2002), Rhizoctonia solani Kühn (Krechel et al 2002;Cao et al 2004b;Coombs et al 2004), Plectosporium tabacinum (Beyma) Palm et al (El-Tarabily 2003), Gauemannomyces graminis var.…”

Section: Introductionmentioning

confidence: 98%

“…(Krechel et al 2002), Rhizoctonia solani Kühn (Krechel et al 2002;Cao et al 2004b;Coombs et al 2004), Plectosporium tabacinum (Beyma) Palm et al (El-Tarabily 2003), Gauemannomyces graminis var. tritici Walker (Coombs et al 2004;Franco et al 2007) and Pythium spp. (Franco et al 2007;El-Tarabily et al 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Performance of three endophytic actinomycetes in relation to plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the United Arab Emirates

2010

View full text Add to dashboard Cite

In the current study, the performance of three endophytic actinomycetes identified as Actinoplanes campanulatus, Micromonospora chalcea and Streptomyces spiralis previously shown to reduce seedling damping-off, and root and crown rots of mature cucumber (Cucumis sativus) caused by Pythium aphanidermatum in pots under greenhouse conditions were further evaluated to determine their potential as biological control agents and as plant growth promoters in the field under the conditions of commercial production of cucumbers in the United Arab Emirates (UAE). When applied individually or in combination to cucumber seedlings, the three isolates significantly promoted plant growth and yield and reduced seedling damping-off and root and crown rots of mature cucumber plants. Individually the performance level of S. spiralis was relatively the best followed by A. campanulatus and then by M. chalcea. The three isolates (which were not inhibitory to each other) performed better, both as biological control agents as well as plant growth promoters, when applied together than when they were inoculated individually. The ability of these three isolates to colonize the internal tissues of roots, stems and leaves under field conditions, and to persist up to 8 weeks after seedling inoculation, showed that they can easily adapt to an endophytic habit systemically within healthy cucumber plants. As the three endophytic actinomycete isolates also colonized the rhizosphere and showed outstanding rhizosphere competency it is clear that they are facultative and not obligate endophytes. The success with the three inoculants indicated that they could well be used in place of the fungicide metalaxyl which is currently recommended for the management of Pythium diseases in the UAE. This is the first successful field use of endophytic actinomycetes as promising plant growth promoters and biological control agents against Pythium diseases of cucumber.

show abstract

“…Although it is located within the plant tissues, its development is also accustomed by both biotic and abiotic factors from within and outside the plant. Consecutively it is able to elicit physiological changes that modify the growth and development of its host [7,8].…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

Widiantini¹,

Herdiansyah²,

Yulia³

2017

KLS

View full text Add to dashboard Cite

Isolation was attempted to collect endophytic bacteria as potential biocontrol agents against rice blast disease (Pyricularia oryzae Cav.). The disease is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led to the searching of alternative controlling method to replace the commonly used pesticide-based method. Endophytic bacteria are bacteria that have intimate relationship with their host without inducing any pathogenic symptom.The use of endophytic microbial as biocontrol agent has its own advantages as the microbes are more easily to adapt to the environment needed by the host plant. We evaluated endophytic bacteria isolated from healthy rice plants and tested for their potential biocontrol activity using dual culture assay. Ten isolates were found to inhibit the growth of P. oryzae of more than 50%. Microscopic observation showed that they were able to cause the mycelia malformation of P. oryzae. Further work is currently in progress to determine their effectiveness in the pot trial.

show abstract

Actinobacterial endophytes for improved crop performance

Cited by 69 publications

References 32 publications

Isolation, Diversity, and Antimicrobial Activity of Rare Actinobacteria from Medicinal Plants of Tropical Rain Forests in Xishuangbanna, China

Isolation, Diversity, and Antimicrobial Activity of Rare Actinobacteria from Medicinal Plants of Tropical Rain Forests in Xishuangbanna, China

Performance of three endophytic actinomycetes in relation to plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the United Arab Emirates

Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

Contact Info

Product

Resources

About