2012
DOI: 10.4049/jimmunol.1103285
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Activated Mouse B Cells Lack Expression of Granzyme B

Abstract: Recently, it has been reported that human B cells express and secrete the cytotoxic protease granzyme B (GrB) after stimulation with IL-21 and BCR cross-linking. To date, there are few clues on the function of GrB in B cell biology. As experimental transgenic murine systems should provide insights into these issues, we assayed for GrB in C57BL/6 B cells using an extensive array of physiologically relevant stimuli but were unable to detect either GrB expression or its proteolytic activity, even when Ag-specific… Show more

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Cited by 31 publications
(25 citation statements)
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“…Furthermore, GrB-secreting B cells possess cytotoxic potential in the absence of perforin expression (64), which is thought to contribute to their immunoregulatory potential (66). In contrast, Hagn et al (67) could not detect GrB expression in resting or activated murine B cells.…”
Section: Unresolved Questionsmentioning
confidence: 90%
“…Furthermore, GrB-secreting B cells possess cytotoxic potential in the absence of perforin expression (64), which is thought to contribute to their immunoregulatory potential (66). In contrast, Hagn et al (67) could not detect GrB expression in resting or activated murine B cells.…”
Section: Unresolved Questionsmentioning
confidence: 90%
“…This is mostly due to the fact that activated mouse B cells lack GrzB [39], suggesting that GrzB biology may not be directly translated from one species to another. Thus, we will need to develop novel in vivo systems to understand fully the role of GrzB in PC development and function in humans.…”
Section: Discussionmentioning
confidence: 99%
“…In vivo immunization and detection of NP-specific antibody via enzyme-linked immunosorbent assay was performed as described in Hagn et al 41 Cell culture of mouse B cells. Mouse B cells were prepared, CFSE labelled and cultured as described in Hawkins et al 37 Cell numbers were determined by reference to fluorescence-activated cell sorting calibration beads as described in Hawkins et al 20 B cells were typically 495% B220 þ , CD19 þ , IgM þ , IgD þ as determined by flow cytometry.…”
Section: Methodsmentioning
confidence: 99%