Activated platelet-derived microparticle numbers are elevated in patients with severe fungal (Candida albicans) sepsis

Woth, Gábor; Tökés-Füzesi, Margit; Magyarlaki, T.; Kovaćs, Gábor L.; Vermes, I.; Mühl, Diána

doi:10.1258/acb.2012.011215

Cited by 45 publications

(34 citation statements)

References 28 publications

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“…However, PDMP assays may consist of quantifying assays, functional assays and morphologic assays. Although the ISTH report was a first step towards achieving standardization of assay methods for evaluating PDMPs, studies continue to use different assay types [24][25][26][27][28][29][30][31][32] . Strasser and coworkers showed a good correlation among three different methods of characterizing PDMPs and suggested that an analysis method other than FCM should be used concurrently to detect MPs when the PDMP size is below the limit of detection on FCM 24) .…”

Section: Discussionmentioning

confidence: 99%

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Association of the Plasma Platelet-Derived Microparticles to Platelet Count Ratio with Hospital Mortality and Disseminated Intravascular Coagulopathy in Critically Ill Patients

Ohuchi

Fujino

Kishimoto

et al. 2015

J Atheroscler Thromb

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Aim:The role of platelet-derived microparticles (PDMPs) in the crosstalk between coagulopathy and inflammation in critically ill patients remains unclear. The aim of this cohort observational study was to investigate the associations between the PDMP levels and hospital mortality or disseminated intravascular coagulopathy (DIC). Methods: This study included 119 patients who were admitted to the ICU. The PDMP levels were measured using an enzyme-linked immunosorbent assay three times a week, for a total of 372 samples. We calculated the maximum (max) PDMP value, max PDMP/platelet (PDMP/Plts) ratio (converted to the PDMP levels per 10 4 platelets) and nadir platelet count during the ICU stay. Baseline patient data and scores, including the Japanese Association for Acute Medicine (JAAM) DIC score, were collected, and potential predictors were analyzed for possible associations with hospital mortality. Results: The max PDMP/Plts ratio was significantly different comparing the survivors (n 98: median, 2.54) and non-survivors (n 21: median 17.59; p 0.001). There was a weak but statistically significant negative correlation between the max PDMP level and nadir platelet count (r 0.332, p 0.001). The max PDMP level and max PDMP/Plts ratio were higher in the DIC group (81.48 and 9.27, respectively) than in the non-DIC group (34.88 and 2.35, p 0.001 and p 0.001, respectively). The max PDMP/Plts ratio was the only variable found to be independently associated with hospital mortality according to a multivariate logistic regression analysis. Conclusions: PDMPs are involved in the development of DIC but are not related to hospital mortality. There is a good association between the PDMP/Plts ratio and hospital mortality and/or DIC in critically ill patients.

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Section: Discussionmentioning

confidence: 99%

“…Woth and coworkers showed that different surface antigens of PDMPs are elevated in subjects with or without fungal infection (CD42a) 28) . Therefore, the measurement method recommended by the ISTH, which uses only a single antigen (CD41), might be too limited for assessing PDMPs.…”

Section: Max Pdmp/plts Ratio For Predicting Hospital Mortalitymentioning

confidence: 99%

Association of the Plasma Platelet-Derived Microparticles to Platelet Count Ratio with Hospital Mortality and Disseminated Intravascular Coagulopathy in Critically Ill Patients

Ohuchi

Fujino

Kishimoto

et al. 2015

J Atheroscler Thromb

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“…For Candida albicans , it is known that this species interacts with platelets in vitro [19, 20], and high numbers of microparticles shed from activated platelets are detected in patients with severe Candida sepsis [21]. Candida albicans strains that are susceptible in vitro to platelet-derived microbicidal peptides (PMP) show decreased virulence in a rabbit model [22].…”

Section: Introductionmentioning

confidence: 99%

Candida: Platelet Interaction and Platelet Activity in vitro

Eberl¹,

Speth²,

Jacobsen³

et al. 2018

J Innate Immun

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Over the last 2 decades, platelets have been recognized as versatile players of innate immunity. The interaction of platelets with fungal pathogens and subsequent processes may critically influence the clinical outcome of invasive mycoses. Since the role of platelets in Candida infections is poorly characterized and controversially discussed, we studied interactions of human platelets with yeast cells, (pseudo-)hyphae, biofilms and secretory products of human pathogenic Candida species applying platelet rich plasma and a whole blood model. Incubation of Candida with platelets resulted in moderate mutual interaction with some variation between different species. The rate of platelets binding to Candida (pseudo-) hyphae and candidal biofilm was comparably low as that to the yeast form. Candida-derived secretory products did not affect platelet activity – neither stimulatory nor inhibitory. The small subset of platelets that bound to Candida morphotypes was consequently activated. However, this did not result in reduced growth or viability of the different Candida species. A whole blood model simulating in vivo conditions confirmed platelet activation in the subpopulation of Candida-bound platelets. Thus, the inability of platelets to efficiently react on Candida presence might favor fungal survival in the blood and contribute to high morbidity of Candida sepsis.

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“…In neutropenic patients the base line platelet count is one predictive parameter for invasive aspergillosis [156] and thrombo cytopenia in liver transplant recipients increases the risk for fungal infections [157]. Elevated numbers of plateletderived microparticles, as found during severe sepsis caused by C. albicans [158], further underline the relevance of these blood cells in fighting fungal infections. Recent insights in the host-pathogen interplay during fungal infec tions have revealed diverse relations between platelets and infectious fungal propagules.…”

Section: Platelets In Fungal Infectionsmentioning

confidence: 99%

Platelets as Immune Cells in Infectious Diseases

et al. 2013

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Platelets have been shown to cover a broad range of functions. Besides their role in hemostasis, they have immunological functions and thus participate in the interaction between pathogens and host defense. Platelets have a broad repertoire of receptor molecules that enable them to sense invading pathogens and infection-induced inflammation. Consequently, platelets exert antimicrobial effector mechanisms, but also initiate an intense crosstalk with other arms of the innate and adaptive immunity, including neutrophils, monocytes/macrophages, dendritic cells, B cells and T cells. There is a fragile balance between beneficial antimicrobial effects and detrimental reactions that contribute to the pathogenesis, and many pathogens have developed mechanisms to influence these two outcomes. This review aims to highlight aspects of the interaction strategies between platelets and pathogenic bacteria, viruses, fungi and parasites, in addition to the subsequent networking between platelets and other immune cells, and the relevance of these processes for the pathogenesis of infections.

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Activated platelet-derived microparticle numbers are elevated in patients with severe fungal (Candida albicans) sepsis

Abstract: The measurement of CD42a- and PAC1-positive microparticles may provide important additional information which can help to improve the early instalment of antifungal therapy of severe septic patients.

Cited by 45 publications

References 28 publications

Association of the Plasma Platelet-Derived Microparticles to Platelet Count Ratio with Hospital Mortality and Disseminated Intravascular Coagulopathy in Critically Ill Patients

Association of the Plasma Platelet-Derived Microparticles to Platelet Count Ratio with Hospital Mortality and Disseminated Intravascular Coagulopathy in Critically Ill Patients

<b><i>Candida</i></b>: Platelet Interaction and Platelet Activity in vitro

Platelets as Immune Cells in Infectious Diseases

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