Activation of Dopamine D1 Receptors in Dermal Fibroblasts Restores Vascular Endothelial Growth Factor-A Production by These Cells and Subsequent Angiogenesis in Diabetic Cutaneous Wound Tissues

Chakroborty, Debanjan; Sarkar, Chandrani; Lu, Kai; Bhat, Madhavi; Dasgupta, Partha; Basu, Sujit

doi:10.1016/j.ajpath.2016.05.008

Cited by 16 publications

(27 citation statements)

References 46 publications

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“…Images were taken using an Axio Scope upright light microscope (Carl Zeiss, Oberkochen, Germany) and compared. Microvessel density (CD31 expression) or angiogenesis was quantitated by analyzing 10 random fields/section [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…HSMEC lysates were collected and used for Western blot assays to determine the expression of phospho-extracellular signal-regulated kinases 1/2 (p-Erk1/2) (0.19 μg/ml) (Cat# 9101, Cell Signaling Technology, 1:1000), total Erk1/2 (0.019 μg/ml) (Cat# 9102, Cell Signaling Technology, 1:1000), phospho-Akt (p-Akt) (0.01 μg/ml) (Cat# 9271, Cell Signaling Technology, 1:1000), total Akt (0.034 μg/ml) (Cat# 9272, Cell Signaling Technology, 1:1000), phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) (0.033 μg/ml) (Cat# 4511, Cell Signaling Technology, 1:1000), and total p38 MAPK (0.023 μg/ml) (Cat# 9212, Cell Signaling Technology, 1:1000). Finally, densitometry was used to quantitate the antibody-reactive bands [ 14 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

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Chebulinic acid is a safe and effective antiangiogenic agent in collagen-induced arthritis in mice

Iwenofu

Mitra

et al. 2020

Arthritis Res Ther

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Background Although vascular endothelial growth factor-A (VEGF)-induced angiogenesis has been reported to play an important role in the pathogenesis of rheumatoid arthritis (RA), serious side effects, mainly grade 2–3 hypertension, which is commonly observed with currently available anti-VEGF agents, can be detrimental for RA patients due to hypertension and associated cardiovascular complications seen in these patients. Thus, identification of anti-VEGF molecules that do not increase blood pressure could be useful for the treatment of RA. Chebulinic acid (CI), a water-soluble small-molecule tannin, can inhibit the actions of VEGF, and a report suggested that CI might not increase blood pressure due to its compensatory effects on the cardiovascular system. Therefore, the effects of CI on blood pressure in mice and the progression of the disease in a murine collagen-induced arthritis (CIA) model were investigated. Methods CIA was induced in DBA/1J mice with type II collagen. The effects of CI in these animals were then evaluated by determination of clinical, histopathological, and immunohistochemical parameters. The effects of CI on VEGF-induced proangiogenic genes and signaling pathways were examined in vitro and in vivo. Results Significant CD31 and VEGF expressions were detected in the synovial tissues of mice with CIA, similar to their expressions observed in human RA patients. However, treatment with CI significantly inhibited paw swelling, decreased the mean articular index and joint pathology scores in these animals through inhibition of VEGF-induced proangiogenic gene expressions and signaling pathways that regulate angiogenesis. Unlike currently used antiangiogenic agents, CI at a dose that inhibits VEGF actions did not increase blood pressure in mice. Conclusion CI can act as a safe and potent anti-VEGF antiangiogenic agent for the treatment of types of inflammatory arthritis, such as RA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Chebulinic acid is a safe and effective antiangiogenic agent in collagen-induced arthritis in mice

Iwenofu

Mitra

et al. 2020

Arthritis Res Ther

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“…As opposed to receptor antagonism, the role of direct activation of the D 1 receptor in wound healing and angiogenesis was evaluated by Chakroborty et al [ 9 ]. Excisional wounds in streptozotocin-induced (type 1 model) diabetic mice healed more quickly in animals treated for five days with specific D 1 receptor agonists (SKF38393 or SKF81297), as compared to controls.…”

Section: Influence On Angiogenesis and Dermal Wound Healingmentioning

confidence: 99%

“…DA receptors are also expressed on various cell types within the skin [ 8 , 9 , 10 ]. Fuziwara, et al demonstrated that DA plays a role in murine skin barrier homeostasis by acting on D 2 receptors that are present on epidermal keratinocytes, resulting in changes in their cAMP level that regulate barrier function [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…This study found differential expression of the D2 and D4 receptors within the basal and outermost layers of the epidermis [ 11 ]. Other work has demonstrated the presence of D 1 receptors in dermal fibroblasts [ 9 ], and D 2 receptors in endothelial cells [ 12 ]. Dopamine reaches the skin primarily via nerve endings, but there is also evidence to suggest that keratinocytes express enzymes to both synthesize and metabolize dopamine [ 11 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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A Concise Review of the Conflicting Roles of Dopamine-1 versus Dopamine-2 Receptors in Wound Healing

et al. 2017

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Catecholamines play an important regulatory role in cutaneous wound healing. The exact role of dopamine in human epidermis has yet to be fully elucidated. Current published evidence describes its differential effects on two separate families of G protein coupled receptors: D1-like and D2-like dopamine receptors. Dopamine may enhance angiogenesis and wound healing through its action on dopamine D1 receptors, while impairing wound healing when activating D2 receptors. This review summarizes the evidence for the role of dopamine in wound healing and describes potential mechanisms behind its action on D1 versus D2-like receptors in the skin.

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Suppression of beta 2 adrenergic receptor actions prevent UVB mediated cutaneous squamous cell tumorigenesis through inhibition of VEGF‐A induced angiogenesis

Bhat

Peters

et al. 2021

Molecular Carcinogenesis

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Although beta 2 adrenergic receptors (β2ADR) are present in the keratinocytes, their role in cutaneous squamous cell tumorigenesis needs to be ascertained. For the first time, we report here that selective β2ADR antagonists by inhibiting β2ADR actions significantly retarded the progression of ultraviolet B (UVB) induced premalignant cutaneous squamous cell lesions. These antagonists acted by inhibiting vascular endothelial growth factor‐A (VEGF) mediated angiogenesis to prevent UVB radiation‐induced squamous cell carcinoma of the skin.

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Activation of Dopamine D1 Receptors in Dermal Fibroblasts Restores Vascular Endothelial Growth Factor-A Production by These Cells and Subsequent Angiogenesis in Diabetic Cutaneous Wound Tissues

Cited by 16 publications

References 46 publications

Chebulinic acid is a safe and effective antiangiogenic agent in collagen-induced arthritis in mice

Chebulinic acid is a safe and effective antiangiogenic agent in collagen-induced arthritis in mice

A Concise Review of the Conflicting Roles of Dopamine-1 versus Dopamine-2 Receptors in Wound Healing

Suppression of beta 2 adrenergic receptor actions prevent UVB mediated cutaneous squamous cell tumorigenesis through inhibition of VEGF‐A induced angiogenesis

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