Active carbon uptake in Laminaria digitata and L. saccharina (Phaeophyta) is driven by a proton pump in the plasma membrane

Klenell, Markus; Snoeijs, Pauli; Pedersén, Marianne

doi:10.1007/978-94-017-0920-0_4

Cited by 18 publications

(20 citation statements)

References 20 publications

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“…These results are in agreement with the photosynthetic rates measured at pH 9.5 at constant DIC, which showed positive values in S. polyschides and L. ochroleuca but zero in the two species of Phyllariopsis. In particular, P. brevipes showed an overall mean pH c of 9.01, similar to that reported before for P. purpurascens (Flores- Moya and Fernández 1998); a pH c of 9.55 was found in L. ochroleuca, in the range of those described for other Laminaria species (9.2-9.76; Surif and Raven 1989;Maberly 1990;2000, Klenell et al 2004), whereas S. polyschides showed a pH c of 9.78. Interestingly, pH c …”

Section: Discussionsupporting

confidence: 81%

“…The facilitation of uncatalyzed HCO 3 -dehydration by the activity of a putative H ? -pump has also been proposed to explain the inorganic carbon uptake in the same species of Laminaria (Axelsson et al 2000;Klenell et al 2004). However, this mechanism would be inhibited by low concentrations of buffers, which has not been observed in these species, at least in the case of Tris buffer (data not shown).…”

Section: Phyllariopsis Purpurascensmentioning

confidence: 95%

“…The higher capacity in the use of HCO 3 -in S. polyschides and L. ochroleuca suggests that other mechanisms, apart from the presence of CA ext , could be present. In fact, the direct uptake of HCO 3 -at pH 9.5 has been proposed for some species of Laminaria, such as Saccharina latissima (sub L. saccharina) and L. digitata (Klenell et al 2004). Moreover, in the giant kelp Macrocystis pyrifera the contribution of an anion exchange protein for the uptake of HCO 3 -seems to be the main inorganic carbon acquisition system regardless of pH (Fernández et al 2014.…”

Section: Phyllariopsis Purpurascensmentioning

confidence: 96%

“…The most widespread mechanism for the use of HCO 3 -appears to be its conversion into CO 2 catalysed by an extracellular carbonic anhydrase (CA ext ; Surif and Raven 1989;Björk et al 1992;Haglund et al 1992a, b;Mercado et al 1996;FloresMoya and Fernández 1998;Zou et al 2003Zou et al , 2011, which is inhibited by acetazolamide (AZ). This mechanism has been proposed to be facilitated in some seaweeds because of the presence of putative acidic zones in the periplasmic space (Axelsson et al 2000;Klenell et al 2002Klenell et al , 2004Mercado et al 2006) due to the activity of a putative H ? -pump at the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

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Photosynthetic use of inorganic carbon in deep-water kelps from the Strait of Gibraltar

García‐Sánchez¹,

Huertas

Fernández³

et al. 2015

Photosynth Res

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Mechanisms of inorganic carbon assimilation were investigated in the four deep-water kelps inhabiting sea bottoms at the Strait of Gibraltar; these species are distributed at different depths (Saccorhiza polysiches at shallower waters, followed by Laminaria ochroleuca, then Phyllariopsis brevipes and, at the deepest bottoms, Phyllariopsis purpurascens). To elucidate the capacity to use HCO3(-) as a source of inorganic carbon for photosynthesis in the kelps, different experimental approaches were used. Specifically, we measured the irradiance-saturated gross photosynthetic rate versus pH at a constant dissolved inorganic carbon (DIC) concentration of 2 mM, the irradiance-saturated apparent photosynthesis (APS) rate versus DIC, the total and the extracellular carbonic anhydrase (CAext), the observed and the theoretical photosynthetic rates supported by the spontaneous dehydration of HCO3(-) to CO2, and the δ(13)C signature in tissues of the algae. While S. polyschides and L. ochroleuca showed photosynthetic activity at pH 9.5 (around 1.0 µmol O2 m(-2) s(-1)), the activity was close to zero in both species of Phyllariopsis. The APS versus DIC was almost saturated for the DIC values of natural seawater (2 mM) in S. polyschides and L. ochroleuca, but the relationship was linear in P. brevipes and P. purpurascens. The four species showed total and CAext activities but the inhibition of the CAext originated the observed photosynthetic rates at pH 8.0 to be similar to the theoretical rates that could be supported by the spontaneous dehydration of HCO3(-). The isotopic (13)C signatures ranged from -17.40 ± 1.81 to -21.11 ± 1.73 ‰ in the four species. Additionally, the δ(13)C signature was also measured in the deep-water Laminaria rodriguezii growing at 60-80 m, showing even a more negative value of -26.49 ± 1.25 ‰. All these results suggest that the four kelps can use HCO3(-) as external carbon source for photosynthesis mainly by the action of external CAext, but they also suggest that the species inhabiting shallower waters show a higher capacity than the smaller kelps living in deeper waters. In fact, the photosynthesis in the two Phyllariopsis species could be accomplished by the spontaneous dehydration of HCO3(-) to CO2. These differences in the capacity to use HCO3(-) in photosynthesis among species could be important considering the increasing levels of atmospheric CO2 predicted for the near future.

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Section: Discussionsupporting

confidence: 81%

Section: Phyllariopsis Purpurascensmentioning

confidence: 95%

Section: Phyllariopsis Purpurascensmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Photosynthetic use of inorganic carbon in deep-water kelps from the Strait of Gibraltar

García‐Sánchez¹,

Huertas

Fernández³

et al. 2015

Photosynth Res

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“…It has been further shown that stimulation of photosynthesis of brown algae by blue light is correlated with an increased inorganic carbon uptake (Klenell et al 2002(Klenell et al , 2004Dring 1993a, b, 1996a, b;Schmid et al 1996). The degree of blue light stimulation is absolutely dependent on the CO 2 concentration of the medium (Forster and Dring 1992).…”

Section: Discussionmentioning

confidence: 97%

Effect of blue light on indoor seedling culture of Saccharina japonica (Phaeophyta)

et al. 2010

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Saccharina japonica is a brown alga that has been commercially cultured on a large scale in China. Integrating the light condition under seawater and the adaptation of Saccharina to this condition, it is expected that blue light would be beneficial to Saccharina culture system. Consequently, the detailed effect of blue light on the key stages during indoor seedling culture of S. japonica was investigated in this study. Irradiances and light qualities had little effect on zoospore attachment and germination. Egg formation occurred sooner under blue light than white light. Under optimum irradiances, 95±4% female gametophytes gave rise to eggs in 6 d under blue light, while it took 12 d for over 90% formation of eggs under white light. Over a culture period of 3 weeks, mean sporeling length and width under blue light was 1.39 and 1.56 times of that under white light, respectively, while the mean sporeling size obtained under red light was only 25% of that under white light. The higher growth rate under blue light was largely due to higher photosynthetic efficiency, as indicated by chlorophyll fluorescence of photosystem II. In addition, the mean ratio of sporeling width to length under blue light was significantly higher than that under white light. These results suggest that blue light would be superior to white light for indoor seedling culture of S. japonica. Based on these findings, an improved S. japonica seedling culture system is discussed.

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Kelps and Environmental Changes in Kongsfjorden: Stress Perception and Responses

Bischof

Buschbaum

Fredriksen

et al. 2019

Advances in Polar Ecology

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On rocky substrata along shallow water cold-temperate and Arctic coastlines, large brown seaweeds ("kelps") form structure-and organism-rich habitats of vast ecological significance. The distribution of these ecosystem engineers is largely controlled by the prevailing temperature, light regime and substrate availability, but can also be influenced by biotic interactions within the kelp communities. In Kongsfjorden, right in the transition of an Arctic to an Atlantic fjord system, the

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Active carbon uptake in Laminaria digitata and L. saccharina (Phaeophyta) is driven by a proton pump in the plasma membrane

Cited by 18 publications

References 20 publications

Photosynthetic use of inorganic carbon in deep-water kelps from the Strait of Gibraltar

Photosynthetic use of inorganic carbon in deep-water kelps from the Strait of Gibraltar

Effect of blue light on indoor seedling culture of Saccharina japonica (Phaeophyta)

Kelps and Environmental Changes in Kongsfjorden: Stress Perception and Responses

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