Active phase prebiotic feeding alters gut microbiota, induces weight-independent alleviation of hepatic steatosis and serum cholesterol in high-fat diet-fed mice

Ghosh, Shreya; Yang, Yifan; Wang, Linghua; Zhang, Chenhong; Zhao, Liping

doi:10.1016/j.csbj.2020.12.011

Cited by 19 publications

(11 citation statements)

References 70 publications

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“…As reported in a previous article, the decreased abundance of Akkermansia was related to severe appendicitis. Recently, Interventions by different means lead to an increased abundance of Akkermansia, which can inhibit the development of diseases in mice or humans ( Bouman and Kaur, 2021 ; Fan et al, 2021 ; Ghosh et al, 2021 ; Liu et al, 2021 ; Luo et al, 2021 ; Tsutsumi et al, 2021 ). This implies that Akkermansia may have initiated a probiotic effect.…”

Section: Discussionmentioning

confidence: 99%

Differences in the Establishment of Gut Microbiota and Metabolome Characteristics Between Balb/c and C57BL/6J Mice After Proton Irradiation

Sui

Zhao

et al. 2022

Front. Microbiol.

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Although proton irradiation is ubiquitous in outer space as well as in the treatment of human diseases, its effects remain largely unclear. This work aimed to investigate and compare the composition of gut microbiota composition of mice in different species exposed to high-dose radiation. Male Balb/c mice and C57BL/6J mice were irradiated at a high dose (5Gy). Fecal specimens before and after irradiation were subjected to high-throughput sequencing (HTS) for the amplification of 16S rRNA gene sequences. We observed substantial changes in gut microbial composition among mice irradiated at high doses compared to non-irradiated controls. The changes included both the alpha and beta diversities. Furthermore, there were 11 distinct alterations in the irradiation group compared to the non-radiation control, including the families Muribaculaceae, Ruminococcaceae, Lactobacillus, Lachnospiraceae_NK4A136, Bacteroides, Alistipes, Clostridiales, Muribaculum, and Alloprevotella. Such alterations in the gut microbiome were accompanied by alterations in metabolite abundances, while at the metabolic level, 32 metabolites were likely to be potential biomarkers. Some alterations may have a positive effect on the repair of intestinal damage. Simultaneously, metabolites were predicted to involve multiple signal pathways, such as Urea Cycle, Ammonia Recycling, Alpha Linolenic Acid and Linoleic Acid Metabolism, Ketone Body Metabolism, Aspartate Metabolism, Phenylacetate Metabolism, Malate-Aspartate Shuttle, Arginine and Proline Metabolism and Carnitine Synthesis. Metabolites produced by proton irradiation in the microbial region play a positive role in repairing damage, making this area worthy of further experimental exploration. The present work offers an analytical and theoretical foundation to investigate how proton radiation affects the treatment of human diseases and identifies potential biomarkers to address the adverse effects of radiation.ImportanceThe space radiation environment is extremely complex, protons radiation is still the main component of space radiation and play an important role in space radiation. We proposed for the first time to compare the feces of Balb/c and C57BL/6J mice to study the changes of intestinal flora before and after proton irradiation. However, the effect of proton irradiation on the gut microbiome of both types of mice has not been previously demonstrated. After proton irradiation in two kinds of mice, we found that the characteristics of intestinal microbiome were related to the repair of intestinal injury, and some metabolites played a positive role in the repair of intestinal injury.

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Section: Discussionmentioning

confidence: 99%

Differences in the Establishment of Gut Microbiota and Metabolome Characteristics Between Balb/c and C57BL/6J Mice After Proton Irradiation

Sui

Zhao

et al. 2022

Front. Microbiol.

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“…The quality control and sequence filtering of raw reads was performed according to the barcode matching and sequence overlapping using QIIME (version 1.9.1). The filtered high-quality reads were clustered into operational taxonomic units (OTUs) with a similarity level of 97%, then the obtained OTUs were used for taxonomical assignments by RDP classifier ( Chen et al., 2019 ; Ghosh et al., 2021 ). The microbial diversities and taxonomic compositions of the sampled bacterial communities were implemented using the R package software ( Zhang et al., 2015 ).…”

Section: Methodsmentioning

confidence: 99%

High-fiber diet ameliorates gut microbiota, serum metabolism and emotional mood in type 2 diabetes patients

Chen

Liu

Ren

et al. 2023

Front. Cell. Infect. Microbiol.

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Previous studies have demonstrated that patients with type 2 diabetes mellitus (T2DM) often had the problems of fecal microbiota dysbiosis, and were usually accompanied with psychiatric comorbidities (such as depression and anxiety). Here, we conducted a randomized clinical study to analyze the changes in gut microbiota, serum metabolism and emotional mood of patients with T2DM after consumption of a high-fiber diet. The glucose homeostasis of participants with T2DM was improved by the high-fiber diet, and the serum metabolome, systemic inflammation and psychiatric comorbidities were also altered. The increased abundances of Lactobacillus, Bifidobacterium and Akkermansias revealed that the proportions of beneficial gut microbes were enriched by the high-fiber diet, while the abundances of Desulfovibrio, Klebsiella and other opportunistic pathogens were decreased. Therefore, the current study demonstrated that the intestinal microbiota alterations which were influenced by the high-fiber diet could improve the serum metabolism and emotional mood of patients with T2DM.

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“…SCFA Analysis. Short-chain fatty acids (SCFAs) were determined by the method of Ghosh et al 24 with a slight modification. Briefly, 0.2 g of intestinal content was added to 0.8 mL of PBS, crushed, and mixed in a mill; then, the solution was centrifuged at 5000 rpm for 20 min, and 50 mL of 50% sulfuric acid solution was added to 0.4 mL of the supernatant for acidification, followed by 500 μL of internal standard solution (ethyl ether solution containing 50 μg/mL of 2methylvaleric acid).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Amelioration Effects and Regulatory Mechanisms of Different Tea Active Ingredients on DSS-Induced Colitis

Wei,

Wang

et al. 2023

J. Agric. Food Chem.

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The potential biological function of tea and its active components on colitis has attracted wide attention. In this study, different tea active ingredients including tea polyphenols (TPPs), tea polysaccharides (TPSs), theabrownin (TB), and theanine (TA) have been compared in the intervention of dextran sulfate sodium (DSS)-induced colitis in mice. Specifically, TPP showed the greatest effect on colitis since it reduced 60.87% of disease activity index (DAI) compared to that of the DSS-induced colitis group, followed by the reduction of 39.13% of TPS and 28.26% of TB on DAI, whereas there was no obvious alleviative effect of TA on colitis. TPP, TPS, and TB could regulate the composition and abundance of gut microbiota to increase the content of short-chain fatty acids (SCFAs) and enhance intestinal barrier function. Further evidence was observed that TPP and TPS regulated the activation of Nrf2/ARE and the TLR4/MyD88/NF-κB P65 pathway to alleviate the colitis. Results of cell experiments demonstrated that TPP showed the greatest antiapoptosis and mitochondrial function protective capability among the tea ingredients via inhibiting the Cytc/Cleaved-caspase-3 signaling pathway. In summary, the superior anticolitis activity of TPP compared to TPS and TB is primarily attributed to its unique upregulation of the abundance of Akkermansia and its ability to regulate the mitochondrial function.

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Active phase prebiotic feeding alters gut microbiota, induces weight-independent alleviation of hepatic steatosis and serum cholesterol in high-fat diet-fed mice

Cited by 19 publications

References 70 publications

Differences in the Establishment of Gut Microbiota and Metabolome Characteristics Between Balb/c and C57BL/6J Mice After Proton Irradiation

Differences in the Establishment of Gut Microbiota and Metabolome Characteristics Between Balb/c and C57BL/6J Mice After Proton Irradiation

High-fiber diet ameliorates gut microbiota, serum metabolism and emotional mood in type 2 diabetes patients

Amelioration Effects and Regulatory Mechanisms of Different Tea Active Ingredients on DSS-Induced Colitis

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