Activity losses among T4 lysozyme charge variants after adsorption to colloidal silica

Bower, C. K.; Sananikone, S.; Bothwell, Michelle Kay; McGuire, Joseph

doi:10.1002/(sici)1097-0290(19990805)64:3<373::aid-bit14>3.0.co;2-j

Cited by 29 publications

(30 citation statements)

References 9 publications

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“…Proteins, including lysozyme, horseradish peroxidase, catalase, and trypsin, adsorb strongly to SiO 2 (sizes ranging from 9 to 40 nm) nanoparticles. These proteins undergo a partial loss of structure and generally a significant loss in enzyme activity (Norde and Anusiem 1992;Kondo et al 1993;Bower et al 1998;Czeslik and Winter 2001).…”

Section: Nanosilica For Medicine and Drug Developmentmentioning

confidence: 99%

Nanosilica—from medicine to pest control

2008

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Nanotechnology is a broad interdisciplinary area of research, development, and industrial activity that has been growing rapidly worldwide for the past decade. More ambitious uses of nanoparticles are bioremediation of contaminated environments, controlled release of fragrances, biocides, and antifungals on textiles. Silica nanocomposites have received much attention because of its thermal degradation behavior and applications in chromatography, medicine, optics, etc. Nanobiotech takes agriculture from the battleground of genetically modified organisms to the brave new world of atomically modified organisms where rice has been modified atomically. Silica has been widely applied in various industries. Application of gold-coated silica has been used in the treatment for benign and malignant tumor. Surface-modified hydrophobic as well as lipophilic nanosilica could be effectively used as novel drugs for treatment of chicken malaria and nuclear polyhedrosis virus (BmNPV), a scourge in silkworm industry. Here, the authors attempt to provide a review to explain the impact of nanosilica on basic biology, medicine, agro-nanoproducts, and use of amorphous nanosilica as biopesticide.

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Section: Nanosilica For Medicine and Drug Developmentmentioning

confidence: 99%

Nanosilica—from medicine to pest control

2008

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“…We used a number of techniques to study the interfacial behavior of charge-changed as well as structural stability mutants of T4 lysozyme, including in situ ellipsometry [20][21][22][23][24], air-water interfacial tensiometry [25,26], radioisotope labeling with 125 I [24] and 14 C [26], circular dichroism (CD) [27,28], the interferometric surface force technique [29], and various assays of enzymatic activity of T4 lysozyme mutants bound to surfaces [30,31]. The elution of adsorbed protein by surfactant had been used by Horbett and coworkers [32][33][34][35][36] to provide an index of protein binding strength, and the earliest work with T4 lysozyme at interfaces was focused on ellipsometric detection of the adsorption and dodecyltrimethylammonium bromide-mediated elution of T4 lysozyme stability variants at hydrophilic and hydrophobic surfaces [20].…”

Section: Measurement Of Protein Adsorption and Binding Strengthmentioning

confidence: 99%

“…Bower et al [30,31] correlated the changes in secondary structure that occurred among T4 lysozyme variants with their corresponding changes in enzymatic activity using two different assays. In one, lyophilized Micrococcus lysodeikticus cells were suspended in phosphate buffer, and the activities of T4 lysozyme as well as T4 lysozyme-nanoparticle complexes were determined by measuring the decrease in turbidity of the Micrococcus substrate as a function of time.…”

Section: Measurement Of Adsorbed T4 Lysozyme Functionmentioning

confidence: 99%

Building a working understanding of protein adsorption with model systems and serendipity

McGuire

2014

Colloids and Surfaces B: Biointerfaces

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“…Lower and coworkers [199] have investigated the adsorption of T4 lysozyme on colloidal silica. It was observed that the enzymatic activity decreased upon adsorption due to the differences in adsorbed enzyme structure and orientation as well as the electrostatic effects.…”

Section: Immobilization In Siomentioning

confidence: 99%

Enzyme‐Based Bioinorganic Materials

Forano

Prévot

2007

Bio‐inorganic Hybrid Nanomaterials

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Trends in research materials currently focus on innovation in the field of intelligent or smart materials [1]. Smart materials are materials that display functionalities able to detect a change in the near environment, answer to a stimulus, modify a property, store, transfer or convert a signal, in a sense behave like living cells. The design of smart materials focuses on the concept of biomimetic materials [2][3][4] in order to reproduce specific and selective properties not deliverable by standard chemical or physical processes. Hence, there has been much effort concentrated on the development of enzyme-based biohybrid materials. Enzyme-based biohybrid materials are under much investigation for the operation of physical or chemical functions. Enzymes display active and selective properties such as molecular recognition, complexation or carrier functions, and a wide range of catalytic reactions (hydrolysis, condensation, redox reaction, isomerization, reaction transfer). Enzymes are thus very attractive as an alternative to traditional catalysts when chemical routes are difficult to apply. Enzymes are able to catalyze regio-and stereo-selective reactions with rate accelerations up to 10-17 fold [17]. They are interesting materials for realization of sensing devices, recognition systems and nanoreactors with many potential industrial developments in biocatalysis [4] for the food industry or effluent treatment [5,6], in biomedical domains for medical implants or enzyme delivery [7], in bioanalysis and affinity chromatography [8,9], and in biosensor technology [10][11][12].Engineering the development of biohybrid enzyme-based materials requires solution of the limitations of the use of enzymes due to their intrinsic fragility against the various environmental stresses and their narrow range of working conditions (e.g. short pH and temperature range). Embedding enzymes in protective matrices in order to solve these problems has been envisaged for many years [13,14]. Immobilization of enzymes in solid supports is seen as a strategy to preserve the active site, to improve the performance of the enzyme, that is, its activity, reaction rate and long-term stability, and to allow enzyme reuse. Physical and chemical properties of the solid supports such as particle size, surface area, pore diameter, mechanical j443 Bio-inorganic Hybrid Nanomaterials. Edited

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Activity losses among T4 lysozyme charge variants after adsorption to colloidal silica

Cited by 29 publications

References 9 publications

Nanosilica—from medicine to pest control

Nanosilica—from medicine to pest control

Building a working understanding of protein adsorption with model systems and serendipity

Enzyme‐Based Bioinorganic Materials

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