Activity, stability and conformational flexibility of seed coat soybean peroxidase

Kamal, J.K. Amisha; Behere, Digambar V.

doi:10.1016/s0162-0134(03)00004-7

Cited by 72 publications

(52 citation statements)

References 44 publications

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“…In addition, SBP shows a remarkable thermal stability, works well in a wide pH range and has a low sensitivity to various organic solvents [20][21]. These features ensure the possibility of creating a very stable system that could be self-maintained by the TiO 2 action.…”

Section: Introductionmentioning

confidence: 99%

TiO2-soybean peroxidase composite materials as a new photocatalytic system

Calza

Avetta

Rubulotta

et al. 2014

Chemical Engineering Journal

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Section: Introductionmentioning

confidence: 99%

TiO2-soybean peroxidase composite materials as a new photocatalytic system

Calza

Avetta

Rubulotta

et al. 2014

Chemical Engineering Journal

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“…acidic pH ranged 5-7 in four different varieties of apple (Dubey et al 2007;Dalal and Gupta 2010;Singh et al 2010). Optimum pH of peroxidases was also reported in case of vegetable sources Estimation of secondary structural elements at various pH values indicated that there is a significant reduction of betastrands and beta-turns at pH 5.5 causing the heme to be further exposed to the solvent and increasing the overall conformational flexibility of the protein (Kamal and Behere 2003).…”

Section: Effect Of Phmentioning

confidence: 78%

Biochemical characterization of Santalum album (Chandan) leaf peroxidase

Kumar¹,

Kamle²,

Singh³

2011

Physiol Mol Biol Plants

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The Santalum peroxidase was extracted from the leaves and precipitated with double volume of chilled acetone. The optimum percent relative activity for the Santalum peroxidase was observed at pH 5.0 and 50°C temperature. The Santalum peroxidase per cent relative activity was stimulated in the presence of phenolic compounds like ferrulic acid and caffeic acids; however, indole-3-acetic acid (IAA) and protocatechuic acid act as inhibitors. All divalent cations Fe 2+ , Mn 2+ , Mg 2+ , Cu 2+ and Zn 2+ stimulate the relative activity of the Santalum peroxidase at concentration of 2.0 μM. Amino acids like L-alanine and L-valine activate the per cent relative activity, while L-proline and DL-methionine showed moderate inhibition for the Santalum peroxidase. However, a very low a concentration of cysteine acts as a strong inhibitor of Santalum peroxidase at the concentration of 0.4 mM. Native polyacrylamide gel electrophoresis (Native-PAGE) was performed for isoenzyme determination and two bands were observed. K m and V max values were calculated from Lineweaver-Burk graph. The apparent V max /K m value for O-dianisidine and H 2 O 2 were 400 and 5.0×105 Units/min/ mL respectively.

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“…189 SBP has advantages over HRP in terms of catalytic activity and stability [55]; these 190 can be exploited in biosensors. Also, unlike HRP, SBP is active in the pH range [2][3][4][5][6]191 offering a greater range of potential biosensing applications [56].…”

Section: Sbp In Biosensors 188mentioning

confidence: 99%

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Ryan

Carolan

Ó’Fágáin

2006

Trends in Biotechnology

141

102

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Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful 9 biotechnological tools. HRP is often termed the classical plant heme peroxidase, and 10 although it has been studied for decades our understanding has deepened since its 11 cloning and subsequent expression, which has enabled numerous mutational and 12 protein engineering studies. SBP, however, has been neglected until recently; despite 13 offering a real alternative to HRP that actually outperforms it in terms of stability. 14 SBP is now used in numerous biotechnological applications, including biosensors. 15Review of both is timely. This article summarises and discusses the main insights into 16 the structure and mechanism of HRP, with special emphasis on HRP mutagenesis, and 17 outlines its use in a variety of applications. It also reviews current knowledge and 18 applications to date of SBP, particularly biosensors. The final paragraphs speculate on 19 the future of plant heme-based peroxidases, with probable trends outlined and 20 explored. 21 22

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Activity, stability and conformational flexibility of seed coat soybean peroxidase

Cited by 72 publications

References 44 publications

TiO2-soybean peroxidase composite materials as a new photocatalytic system

TiO2-soybean peroxidase composite materials as a new photocatalytic system

Biochemical characterization of Santalum album (Chandan) leaf peroxidase

Horseradish and soybean peroxidases: comparable tools for alternative niches?

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