Acute and chronic toxicity assessment and the gene expression of Dhb, Vtg, Arnt, CYP4, and CYP314 in Daphnia magna exposed to pharmaceuticals

Bang, Seung Hyuck; Ahn, Ji‐Young; Hong, Nam-Hui; Sekhon, Simranjeet Singh; Kim, Yang‐Hoon; Min, Jiho

doi:10.1007/s13273-015-0013-7

Cited by 25 publications

(7 citation statements)

References 24 publications

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“…The effects of tetracycline on vitellogenin have been reported in Daphnia and other species. The Daphnia vitellogenin gene was down-regulated under the exposure of 0.9 mg L –1 of tetracycline, and reduced reproduction was also observed . Conversely, vitellogenin was produced in male fish ( O. latipes ) following the exposure of tetracycline in the range of 1 to 20 mg L –1…”

Section: Resultsmentioning

confidence: 99%

Multigenerational Effects of the Antibiotic Tetracycline on Transcriptional Responses of Daphnia magna and Its Relationship to Higher Levels of Biological Organizations

Kim

Asselman

Jeong

et al. 2017

Environ. Sci. Technol.

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Given the risk of environmental pollution by pharmaceutical compounds and the effects of these compounds on exposed ecosystems, ecologically relevant and realistic assessments are required. However, many studies have been mostly focused on individual responses in a single generation exposed to one-effect concentrations. Here, transcriptional responses of the crustacean Daphnia magna to the antibiotic tetracycline across multiple generations and effect concentrations were investigated. The results demonstrated that tetracycline induced different transcriptional responses of daphnids that were dependent on dose and generation. For example, reproduction-related expressed sequence tags (ESTs), including vitellogenin, were distinctly related to the dose-dependent tetracycline exposure, whereas multigenerational exposure induced significant change of molting-related ESTs such as cuticle protein. A total of 65 ESTs were shared in all contrasts, suggesting a conserved mechanism of tetracycline toxicity regardless of exposure concentration or time. Most of them were associated with general stress responses including translation, protein and carbohydrate metabolism, and oxidative phosphorylation. In addition, effects across the dose-response curve showed higher correlative connections among transcriptional, physiological, and individual responses than multigenerational effects. In the multigenerational exposure, the connectivity between adjacent generations decreased with increasing generation number. The results clearly highlight that exposure concentration and time trigger different mechanisms and functions, providing further evidence that multigenerational and dose-response effects cannot be neglected in environmental risk assessment.

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Section: Resultsmentioning

confidence: 99%

Multigenerational Effects of the Antibiotic Tetracycline on Transcriptional Responses of Daphnia magna and Its Relationship to Higher Levels of Biological Organizations

Kim

Asselman

Jeong

et al. 2017

Environ. Sci. Technol.

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“…To conduct this experiment, D. magna, which were less than 30days old were collect ed and were exposed to lethal concentration of 50% (LC50) of caffeine, ibuprofen, aspirin and tetracycline for 48 h, respectively 18 . After exposure the chemicals in D. magna, the proteins were isolated for proteomic analysis.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of Daphnia magna exposed to caffeine, ibuprofen, aspirin and tetracycline

Bang

Hong

Ahn

et al. 2015

Toxicol. Environ. Health Sci.

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The toxicity of pharmaceuticals thrown indiscrimina tely adversely affects the environment. In this study, the impact of caffeine, ibuprofen, aspirin and tetra cycline has been examined through toxicity assay based proteomic analysis. Proteomic profile in Daph nia magna was analyzed by twodimensional elec trophoresis with pH in the range 3 to 10. The LC50 of caffeine, ibuprofen, aspirin and tetracycline deter mined as 445.3, 91.5, 310 and 90 mg/L, respectively, was used to expose to D. magna grown for 48 hour before isolating total proteins. The results show that the expression pattern of protein's profile in D. magna gets changed due to the toxicity of caffeine, ibupro fen, aspirin and tetracycline. A large number of pro teins expressed differently in D. magna in compari son to those exposed to various pharmaceuticals. The results showed that 18 up and 1 downregula tion spot in relation to caffeine exposure; 24 up and 6 downregulation spots in relation to ibuprofen exp osure; 3 up and 4 downregulation spots in relation to aspirin exposure and 10 up and 3 downregula tion spots in relation to tetracycline exposure have been observed. Thus, the present systemic approach using proteomic analysis is very helpful in screening novel biomarkers for pharmaceuticals induced haz ardous effects.

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“…SDS-PAGE was carried out at 200 mV, 400 mA for 1 h 20 min at room temperature until the bromophenol blue indicator reached the bottom of the gel. The gels were fixed with fixing buffer (50% methanol, 12% acetic acid, 38% distilled water and 0.00053% formaldehyde) for over 2 hour 9,11 .…”

Section: Two-dimensional Polyacrylamide Gel Electrophoresismentioning

confidence: 99%

Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

Sekhon

Ahn

Yoon

et al. 2015

Toxicol. Environ. Health Sci.

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Acute and chronic toxicity assessment and the gene expression of Dhb, Vtg, Arnt, CYP4, and CYP314 in Daphnia magna exposed to pharmaceuticals

Cited by 25 publications

References 24 publications

Multigenerational Effects of the Antibiotic Tetracycline on Transcriptional Responses of Daphnia magna and Its Relationship to Higher Levels of Biological Organizations

Multigenerational Effects of the Antibiotic Tetracycline on Transcriptional Responses of Daphnia magna and Its Relationship to Higher Levels of Biological Organizations

Proteomic analysis of Daphnia magna exposed to caffeine, ibuprofen, aspirin and tetracycline

Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

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