Acute and sub-chronic toxicological evaluation of the herbal product HAD-B1 in Beagle dogs

Song, Seung Han; Chang, Hyeok-Joon; Kim, Soo-Dam; Kwag, Eun-Bin; Park, So‐Jung; Yoo, Hwa‐Seung

doi:10.1016/j.toxrep.2021.11.002

Cited by 2 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, many authors stated that EOs, nanoemulsion preparations, and oil terpenes were relatively safe when assessed against several nontarget species [3,17,28,69]. In that regard, most reports have focussed on assessing the acute toxicity, whereas both subchronic and chronic evaluations have not been fully undertaken [5,17,[69][70][71]. Regarding cypress oil, health risks or side effects following administration of designated therapeutic dosages are not recorded.…”

Section: Discussionmentioning

confidence: 99%

Cupressus sempervirens Essential Oil, Nanoemulsion, and Major Terpenes as Sustainable Green Pesticides against the Rice Weevil

et al. 2023

View full text Add to dashboard Cite

In order to find effective, biorational, and eco-friendly pest control tools, Cupressus sempervirens var. horizontalis essential oil (EO) was produced using hydrodistillation, before being analyzed with gas chromatography, specifically, using flame ionization detection. The monoterpene components α-pinene (46.3%), δ-3-carene (22.7%), and α-cedrol, a sesquiterpene hydrocarbon, (5.8%), were the main fractions. An oil-in-water nanoemulsion was obtained following a green protocol. The EO, its nanoemulsion, and its terpenes each exhibited both insecticidal and insect repellent activities against the rice weevil, Sitophilus oryzae. In a contact bioassay, the nanoemulsion induced a 100% adult mortality rate in a concentration of 10.0 µL/cm2 after 4 days of treatment, whereas 40 µL/cm2 of EO and α-cedrol was required to kill 100% of weevils. Using fumigation, nanoemulsion and EO at 10 µL/L air caused a 100% adult mortality rate after 4 days of treatment. The LC50 values of botanicals ranged between 5.8 and 53.4 µL/cm2 for contact, and between 4.1 and 19.6 µL/L for fumigation. The phytochemicals strongly repelled the weevil at concentrations between 0.11 and 0.88 µL/cm2, as well as considerably inhibiting AChE bioactivity. They were found to be safe for earthworms (Eisenia fetida) at 200 mg/kg, which also caused no significant alteration in wheat grain viability. This study provides evidence for the potential of using the EO of C. sempervirens and its nanoemulsion as natural, eco-friendly grain protectants against S. oryzae.

show abstract