Acute electric field downregulates human plasma immunoreactive interleukin-6 and -1β levels: Molecular mechanisms underlying inflammation alleviation through electric field therapy

Abstract

Medical treatment using high-voltage electric potential (HELP) devices to generate an electric field (EF) is an alternative therapy commonly used in Japan. However, the mechanisms underlying potential health benefits of this therapy are still unclear. Therefore, we investigated the effect of HELP exposure (9 kV/electrode+9 kV/ electrode, 30 min) on several cytokines and hormones using enzyme-linked immunosorbent assays in plasma samples obtained from healthy human subjects before and after a single treatment session. Immunoreactive interleukin (IL)-1β and IL-6 levels were significantly downregulated following HELP exposure. Under these treatment conditions, HELP exposure did not exert on immunoreactive IL-10, IL-18, transforming growth factor-beta 1 (TGF-β1), tumor necrosis factor-alpha (TNF-α) adrenaline, serotonin, histamine, neuropeptide Y, somatostatin, insulin, or dehydroepiandrosterone sulfate (DHEAS) levels. The activation of transient receptor potential melastatin 8 (TRPM8) induces the suppression of the levels of inflammatory markers. Therefore, we further examined the in silico docking

Targeted lipidomics reveals changes in N-acyl serines by acute exposure to an electric field: Molecular insights into the docking of N-18:1 serine interaction with TRPV1 or PPAR-α

“…As described previously [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], EF exposure comprised of a system that was equipped with a transformer, a seat, and two insulator-covered electrodes. One electrode was placed on the floor plate, near the subject's feet, while the other was placed on the head.…”

“…High-voltage electric field (EF) therapy may be an effective treatment for shoulder stiffness, headache, insomnia, and chronic constipation [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Although EF therapy was discovered about 90 years ago, the molecular mechanisms associated with its health benefits remain elusive.…”

“…Although EF therapy was discovered about 90 years ago, the molecular mechanisms associated with its health benefits remain elusive. A therapeutic device that exposes the human body to high-voltage electric potential (HELP) was approved by the Ministry of Health, Labour and Welfare in Japan [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. A review of the literature suggests that HELP exposure may be an alternative therapy for several conditions.…”

Molecular insights into the docking of N-palmitoyl serine (Palmi-Ser) interaction with the nuclear receptor subfamily 4 group A member 2 (NR4A2): Effect of Palmi-Ser on survival rate in the Drosophila model of Parkinson's disease

“…High-voltage electric field (EF) therapy may be effective in treating shoulder stiffness, headache, insomnia, and chronic constipation [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. Although EF therapy was discovered about 90 years ago, the molecular mechanisms associated with its health benefits remain elusive.…”

“…Although EF therapy was discovered about 90 years ago, the molecular mechanisms associated with its health benefits remain elusive. The Ministry of Health, Labor, and Welfare in Japan approved a therapeutic device that exposes the human body to high-voltage electric potential (HELP) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. A review of the literature suggests that HELP exposure may be an alternative therapy for several conditions.…”