Acute Hypoxia during Organogenesis Affects Cardiac Autonomic Balance in Pregnant Rats

Maslova, M. V.; Граф, А. В.; Маклакова, А. С.; Krushinskaya, Ya. V.; Sokolova, N. A.; Koshelev, V. B.

doi:10.1007/s10517-005-0241-2

Cited by 8 publications

(7 citation statements)

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“…Female rats were exposed to hypobaric hypoxia at 5% O 2 (11500 m altitude, 145 mm Hg) in a decompression (altitude) chamber "Mez Mohelnice" (Mohelnice, Czech Republic) of 3.3 L volume, as described previously [25,26]. Briefly, after closing the chamber, air pressure inside the chamber was progressively decreased during 1 min (200 m/sec) by the vacuum pump connected to the chamber.…”

Section: Acute Hypobaric Hypoxiamentioning

confidence: 99%

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Граф

Trofimova

Ksenofontov

et al. 2020

Cells

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Function of brain amino acids as neurotransmitters or their precursors implies changes in the amino acid levels and/or metabolism in response to physiological and environmental challenges. Modelling such challenges by pregnancy and/or hypoxia, we characterize the amino acid pool in the rat cerebellum, quantifying the levels and correlations of 15 amino acids and activity of 2-oxoglutarate dehydrogenase complex (OGDHC). The parameters are systemic indicators of metabolism because OGDHC limits the flux through mitochondrial TCA cycle, where amino acids are degraded and their precursors synthesized. Compared to non-pregnant state, pregnancy increases the cerebellar content of glutamate and tryptophan, decreasing interdependence between the quantified components of amino acid metabolism. In response to hypoxia, the dependence of cerebellar amino acid pool on OGDHC and the average levels of arginine, glutamate, lysine, methionine, serine, phenylalanine, and tryptophan increase in non-pregnant rats only. This is accompanied by a higher hypoxic resistance of the non-pregnant vs. pregnant rats, pointing to adaptive significance of the hypoxia-induced changes in the cerebellar amino acid metabolism. These adaptive mechanisms are not effective in the pregnancy-changed metabolic network. Thus, the cerebellar amino acid levels and OGDHC activity provide sensitive markers of the physiology-dependent organization of metabolic network and its stress adaptations.

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Section: Acute Hypobaric Hypoxiamentioning

confidence: 99%

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Граф

Trofimova

Ksenofontov

et al. 2020

Cells

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“…Owing to this, we tested if SP would show a protective action on the behavioral and biochemical changes induced by hypoxia. We used the previously established model, in which acute hypoxia was created in a decompression chamber with female rats sensitized to the insult by pregnancy [21, 24, 31]. Protective action of SP was observed already during the acute hypoxia in decompression chamber.…”

Section: Resultsmentioning

confidence: 99%

“…Protective action of SP was observed already during the acute hypoxia in decompression chamber. Within an animal group, the resistance to hypoxia assessed by both physiological and behavioral parameters is known to vary, defined as low, medium, or high when the time before collapsing under hypoxic conditions is less than 5 minutes, between 5 and 10 minutes, and 10 minutes and more, respectively [24, 32]. Figure 4 shows that pre-conditioning with a low doze of SP (5 mg/kg) increased the percentage of the highly resistant animals at the expense of the medium resistant group.…”

Section: Resultsmentioning

confidence: 99%

“…Pregnant rats were exposed to hypobaric hypoxia at day 9–10 of pregnancy by placing in a decompression (altitude) chamber of 3.3 L volume, with a vacuum pump “Mez Mohelnice” (Mohelnice, Czech Republic). Acute hypoxia was achieved by decreasing the atmospheric pressure in 1 min to 145 mm Hg, correspondent to 11500 m altitude [24]. SP was introduced to animals at 5 and 25 mg/kg by intranasal application of the water solution of the trisodium salt, with the physiologic solution substituting for SP in all reference groups.…”

Section: Methodsmentioning

confidence: 99%

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Behavioral Impact of the Regulation of the Brain 2-Oxoglutarate Dehydrogenase Complex by Synthetic Phosphonate Analog of 2-Oxoglutarate: Implications into the Role of the Complex in Neurodegenerative Diseases

Trofimova

Lovat

Groznaya

et al. 2010

International Journal of Alzheimer's Disease

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Decreased activity of the mitochondrial 2-oxoglutarate dehydrogenase complex (OGDHC) in brain accompanies neurodegenerative diseases. To reveal molecular mechanisms of this association, we treated rats with a specific inhibitor of OGDHC, succinyl phosphonate, or exposed them to hypoxic stress. In males treated with succinyl phosphonate and in pregnancy-sensitized females experiencing acute hypobaric hypoxia, we revealed upregulation of brain OGDHC (within 24 hours), with the activity increase presumably representing the compensatory response of brain to the OGDHC inhibition. This up-regulation of brain OGDHC was accompanied by an increase in exploratory activity and a decrease in anxiety of the experimental animals. Remarkably, the hypoxia-induced elevation of brain OGDHC and most of the associated behavioral changes were abrogated by succinyl phosphonate. The antagonistic action of hypoxia and succinyl phosphonate demonstrates potential therapeutic significance of the OGDHC regulation by the phosphonate analogs of 2-oxoglutarate.

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“…Pregnant rats were exposed to hypoxia as described previously [ 35 , 67 ] on the 10th or 20th day of pregnancy. These time points roughly correspond to the first and second trimester of human pregnancy.…”

Section: Methodsmentioning

confidence: 99%

Acute Prenatal Hypoxia in Rats Affects Physiology and Brain Metabolism in the Offspring, Dependent on Sex and Gestational Age

Граф

Maslova

Artiukhov

et al. 2022

IJMS

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Hypoxia is damaging to the fetus, but the developmental impact may vary, with underlying molecular mechanisms unclear. We demonstrate the dependence of physiological and biochemical effects of acute prenatal hypoxia (APH) on sex and gestational age. Compared to control rats, APH on the 10th day of pregnancy (APH-10) increases locomotion in both the male and female offspring, additionally increasing exploratory activity and decreasing anxiety in the males. Compared to APH-10, APH on the 20th day of pregnancy (APH-20) induces less behavioral perturbations. ECG is changed similarly in all offspring only by APH-10. Sexual dimorphism in the APH outcome on behavior is also observed in the brain acetylation system and 2-oxoglutarate dehydrogenase reaction, essential for neurotransmitter metabolism. In view of the perturbed behavior, more biochemical parameters in the brains are assessed after APH-20. Of the six enzymes, APH-20 significantly decreases the malic enzyme activity in both sexes. Among 24 amino acids and dipeptides, APH-20 increases the levels of only three amino acids (Phe, Thr, and Trp) in male offspring, and of seven amino acids (Glu, Gly, Phe, Trp, Ser, Thr, Asn) and carnosine in the female offspring. Thus, a higher reactivity of the brain metabolism to APH stabilizes the behavior. The behavior and brain biochemistry demonstrate sexually dimorphic responses to APH at both gestational stages, whereas the APH effects on ECG depend on gestational age rather than sex.

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Acute Hypoxia during Organogenesis Affects Cardiac Autonomic Balance in Pregnant Rats

Cited by 8 publications

References 0 publications

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Behavioral Impact of the Regulation of the Brain 2-Oxoglutarate Dehydrogenase Complex by Synthetic Phosphonate Analog of 2-Oxoglutarate: Implications into the Role of the Complex in Neurodegenerative Diseases

Acute Prenatal Hypoxia in Rats Affects Physiology and Brain Metabolism in the Offspring, Dependent on Sex and Gestational Age

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