Acute Ischemic Liver Failure in the Rat: A Reproducible Model Not Requiring Portal Decompression

Asakawa, Hiroichi; Jeppsson, Bengt; Mack, Peter J.; Hultberg, Björn; Hägerstrand, Inga; Bengmark, S

doi:10.1159/000129002

Cited by 24 publications

(16 citation statements)

References 5 publications

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“…This method does not require any shunt, thus offering technical simplicity. 21 Ischemiareperfusion injury was produced in the left and median lobes for 45 minutes followed by 120 minutes reperfusion. When zincprotoporphyrin IX, a specific inhibitor of HO, was administered to rats, the drug (10 µmoles/kg body weight) was twice injected subcutaneously at 3 and 16 hours before the start of ischemia.…”

Section: Methodsmentioning

confidence: 99%

Doxorubicin preconditioning: A protection against rat hepatic ischemia-reperfusion injury

Itō

Ozasa²,

Sanada

et al. 2000

Hepatology

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Doxorubicin produces clinically useful responses in a variety of human cancers. However, the toxicity of doxorubicin has limited its usefulness. This side effect is mainly due to the doxorubicin-mediated free radical formation. Administration of doxorubicin (10 mg/kg body weight) to rats intravenously induces heme oxygenase-1 (HO-1) in the liver. The levels of HO-1 protein were first detected at 6 hours and peaked at about 18 to 24 hours after the injection. It is known that HO-1 plays a protective role against the oxidative injury. Therefore, we have examined the protective effect of doxorubicin preconditioning against the hepatic ischemia-reperfusion injury. Partial hepatic ischemia was produced in the left and medium lobes for 45 minutes followed by 120 minutes reperfusion. When low doses of doxorubicin (1 mg/kg body weight) was intravenously administered to rats 2 days before the ischemia, the serum alanine transaminase (ALT) levels in the preconditioning rat were clearly improved compared with those in the rat without preconditioning. Under this situation, zincprotoporphyrin IX, a specific inhibitor of HO-1, was injected subcutaneously to rats at 3 and 16 hours before the ischemia, the ALT levels were not improved by doxorubicin preconditioning. Histopathologic examination also supported these results. Although the HO-1 protein level was fairly low 2 days after the doxorubicin administration, significant amounts of HO-1 protein were detected. Our results indicated that the induction of HO-1 played a protective role against hepatic ischemia-reperfusion injury and that doxorubicin preconditioning is more clinically useful than other preconditioning methods. (HEPATOLOGY 2000;31:416-419.)

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Section: Methodsmentioning

confidence: 99%

Doxorubicin preconditioning: A protection against rat hepatic ischemia-reperfusion injury

Itō

Ozasa²,

Sanada

et al. 2000

Hepatology

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“…Intestinal pooling of blood generates proinflammatory gut metabolites (e.g. tumor necrosis factor ␣ , 5-hydroxytryptamine), which is harmful at reperfusion [7][8][9][10][11] . In order to minimize these factors in ischemia/reperfusion injury (IRI) animal studies, technically demanding and unphysiological portocaval shunt models have been developed [9,10] .…”

Section: Introductionmentioning

confidence: 99%

Segmental Ischemia of the Liver – Microdialysis in a Novel Porcine Model

et al. 2009

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Background: Segmental liver ischemia is often used in rodents to study ischemia and reperfusion injuries (IRI). There are no reports of protocols using segmental ischemia in porcine models. Microdialysis (MD) provides the opportunity to study local effects of IRI in vivo. Methods: Eight pigs received an MD catheter placed in liver segments IV and V, respectively. All circulation to segment IV was stopped for 80 min, and reperfusion was followed for 240 min. Results: During ischemia the levels of lactate, glycerol and glucose increased 3-fold (p < 0.001), 40-fold (p < 0.001) and 4-fold (p < 0.01), respectively, in the ischemic segment compared to the perfused segment, whereas the levels of pyruvate fell to a tenth of the preischemic level (p < 0.001). All values returned to baseline after reperfusion. Serum levels of aspartate aminotransferase increased (p < 0.05). Polymorphonuclear cells increased in both segments, although the density was significantly higher in segment IV. Conclusion: Clamping of one liver segment in pigs is a simple, stable and reproducible model to study IRI with minimal systemic effects. MD revealed no signs of anaerobic metabolism in the perfused segment but still there was an increase in the number of polymorphonuclear neutrophils in this segment, although it was lower than that in the ischemic segment.

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“…De acordo com a literatura consultada, os tempos de isquemia hepática variam de 30 até 100 minutos (Asakava et al, 1989;Isozaki et al, 1995), sendo os maiores períodos relacionados com trabalhos que envolvem isquemia hepática parcial ou total com derivações vasculares, e os menores, com trabalhos que envolvem isquemia total, sem derivações vasculares, como descrito por Sébe (1999) e reproduzido neste estudo.…”

Section: Discussionunclassified

Aspectos morfológicos, morfométricos e ultraestruturais do baço de ratos após o clampeamento total do pedículo hepático

Freitas

Neto

Dória

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliaram-se as alterações morfológicas, morfométricas e ultraestruturais que ocorreram no baço devido à isquemia produzida pelo clampeamento total do pedículo hepático. Para tanto, foram utilizados 40 ratos machos, distribuídos em quatro grupos de 10 animais. O grupo-controle (C) não foi submetido à isquemia, e os grupos tratados (E 1 , E 2 e E 3 ) foram submetidos ao clampeamento por 10, 20 e 30 minutos, respectivamente. Fragmentos do baço foram retirados e analisados histologicamente pela microscopia de luz (hematoxilina-eosina, ferrocianeto-férrico) e pela microscopia eletrônica de transmissão. Os resultados demonstraram que 10 minutos de clampeamento do pedículo hepático são suficientes para apresentar sinais de congestão esplênica e 20 e 30 minutos promovem intensa digestão de hemácias pelos macrófagos, com presença de grânulos de ferro (hemossiderina) no parênquima esplênico.Palavras-chave: rato, baço, clampeamento, pedículo hepático, hemossiderina ABSTRACTThe macro and microscopic alterations that occurred in the spleen during an ischemia produced by the hepatic pedicle total clamping were studied. Forty male rats were distributed in four groups of 10 animals each. The control group (C) was not submitted to ischemia and the treated groups (E 1 , E 2 , and E 3 ) were submitted to the clamping during 10, 20, and 30 minutes, respectively. Spleen fragments were collected and histologically analyzed by the light microscopy (eosin-hematoxilin and ferric ferrocyanide) and by the transmission electron microscopy. The results showed that 10 minutes of hepatic pedicle total clamping was enough produce signs of splenic congestion and 20 and 30 minutes promoted intense red bood cels digestion by the macrophages with the presence of iron granules (hemosiderin) in the splenic parenchyma.

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Acute Ischemic Liver Failure in the Rat: A Reproducible Model Not Requiring Portal Decompression

Cited by 24 publications

References 5 publications

Doxorubicin preconditioning: A protection against rat hepatic ischemia-reperfusion injury

Doxorubicin preconditioning: A protection against rat hepatic ischemia-reperfusion injury

Segmental Ischemia of the Liver – Microdialysis in a Novel Porcine Model

Aspectos morfológicos, morfométricos e ultraestruturais do baço de ratos após o clampeamento total do pedículo hepático

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