2000
DOI: 10.1006/cimm.2000.1725
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Acute Myelogenous Leukemia Blasts as Accessory Cells during in Vitro T Lymphocyte Activation

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Cited by 22 publications
(14 citation statements)
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“…The methods for preparation and characterisation of the clones have been described in detail previously (Bruserud et al 1998;Bruserud and Ulvestad 2000a;Bruserud and Ulvestad 2000b). All the clones expressed the membrane molecule phenotype CD2 + CD4 + CD8 À T cell receptor (TCR) ab + cd À .…”
Section: T Lymphocyte Clonesmentioning
confidence: 99%
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“…The methods for preparation and characterisation of the clones have been described in detail previously (Bruserud et al 1998;Bruserud and Ulvestad 2000a;Bruserud and Ulvestad 2000b). All the clones expressed the membrane molecule phenotype CD2 + CD4 + CD8 À T cell receptor (TCR) ab + cd À .…”
Section: T Lymphocyte Clonesmentioning
confidence: 99%
“…The release of a wide range of CCL and CXCL chemokines was also investigated for Cal72 and SJSA-1 cells (for details, see below). Alternatively, 3 H-thymidine was added after 48 h (37 kBq/well added in 20 ll saline; TRA 310, Amersham International, Amersham, UK) and nuclear radioactivity assayed 18 h later (Bruserud and Ulvestad 2000a;Bruserud and Ulvestad 2000b).…”
Section: Suspension Cultures Of Osteosarcoma Cells Alonementioning
confidence: 99%
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“…Antibodies were diluted in culture medium (0.1 ml antibody diluted up to 12.5 ml, final dilution 1:125) (18), and these stock solutions were stored at 270°C. The anti-CD3 mAb was further diluted to 1:500 and anti-CD28 to 1:250 before used in the experiments (both mAbs then added at 20 ml/well, see below).…”
Section: Reagents For Tissue Culturementioning
confidence: 99%
“…Mononuclear cells were activated by 1 g/ml phytohemagglutinin (PHA; HA16; Wellcome, Dartford, UK) or monoclonal anti-CD3 (mouse IgE Moab; CLB-T3/4.E; The Central Laboratory of The Netherlands Red Cross Blood Transfusion Services, Amsterdam, The Netherlands) as described in. 33 …”
Section: Chemicalsmentioning
confidence: 99%