Acute phase proteins in three healthy antelope species

Baldrey, V.; Verghese, R.; Wernery, Ulrich; Bailey, Tom

doi:10.1136/vr.100272

Cited by 4 publications

(8 citation statements)

References 14 publications

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“…FIB levels in the normal bongo group (342 ± 30 mg/dl) were comparable to Arabian oryx (291 ± 82 mg/dl), impala (250 ± 18 mg/dl), and blackbuck (395 ± 50 mg/dl) ( 25 ). Normal females had lower levels of FIB than males, and this difference was also observed in the clinically abnormal cohort group.…”

Section: Discussionmentioning

confidence: 74%

Protein Electrophoresis and Haptoglobin Values for Captive Bongo (Tragelaphus eurycerus)

et al. 2021

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Serum samples collected from 37 clinically normal bongo (Tragelaphus eurycerus) and 13 abnormal bongo were tested using assays for acute-phase proteins (APPs) and by protein electrophoresis. Abnormal bongo samples (n = 27) had significantly higher levels of fibrinogen (FIB) (p < 0.001) and trending but not significantly increased haptoglobin (HP) (p = 0.07) vs. samples from normal bongo (n = 37). There were no significant differences in values for total white blood cell counts or for any of the fractions determined by protein electrophoresis. Clinically normal female bongo (n = 19) had significantly lower levels of FIB than normal males (n = 18) (p = 0.014), and this observation was also made with samples from the clinically abnormal group (p = 0.004). Many weak to moderate significant correlations were observed with increasing age, including increased globulins, FIB, and HP and decreased albumin-to-globulin (A/G) ratio and albumin. In clinical cases reviewed in this study, mild HP changes categorized this reactant as a minor APP, which contrasts with the major APP classification of HP in the related species of the cow. The preliminary data indicate that the quantitation of these APPs may offer value in assessing inflammation in this species, but additional studies are needed.

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Section: Discussionmentioning

confidence: 74%

Protein Electrophoresis and Haptoglobin Values for Captive Bongo (Tragelaphus eurycerus)

et al. 2021

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“…Full assay validation has not been performed for any APR assay in nondomesticated mammal species, and some studies provide no information about assay validation 16–18,20–29 . When validation has been performed, this usually only includes imprecision and linearity experiments, 22,30–38 sometimes with additional recovery or limit of detection studies 13,39–46 (Tables 1‐4).…”

Section: Methodsmentioning

confidence: 99%

“…A latex agglutination immunoturbidometric method using a mix of rabbit polyclonal and mouse monoclonal anti‐human SAA antibodies (LZ‐SAA, Eiken, Japan) has been used most often and has been minimally or partially validated for measurement of SAA in serum of white‐tailed deer, bottlenose dolphin, Asian and African elephants, Iberian ibex, Florida manatee, and Grant's zebra 14,30,32,36,42,43,53 . This assay has also been used to detect SAA, without any documentation of assay validation procedures, in healthy Arabian oryx, blackbuck, impala, reindeer, and healthy and diseased cheetah, musk ox, and wallaby 3,16,20 . The LZ‐SAA has been reported not to detect SAA in white rhinoceros and sitatunga 3,34 .…”

Section: Methodsmentioning

confidence: 99%

“…Fibrinogen is a minor positive APR and has been measured using various methods in a range of ungulates, manatees, and leopard seals (Table 3). 18,20,34,66 The heat precipitation method and modifications of the Clauss method do not depend on species‐specific antibodies and are therefore recommended over immunologic methods unless full assay validation is performed for antibody‐based methods 67 . Increased concentrations were demonstrated in bongo and white rhinoceros with inflammatory disease 22,34 …”

Section: Methodsmentioning

confidence: 99%

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Acute phase reactants in nondomesticated mammals—A veterinary clinical pathology perspective

Hooijberg

Cray

2022

Veterinary Clinical Pathol

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Acute phase reactants (APRs) have been used to monitor health, diagnose inflammatory disease, and track response to treatment in domesticated animals for several decades. Measurement of APRs has now become part of routine clinical chemistry testing for companion animals in many areas of the world, as earlier reviews have indicated. 1,2 The increased use of APRs in domesticated animals has been paralleled by an increasing number of studies detailing the measurement and application of APRs in nondomesticated mammals.

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“…However, no reports investigating concentration of APPs in European bison are available. Although, some studies have been described in other wildlife non-bovid species, nevertheless the data is very limited [15][16][17]. The secretion of APPs is associated with the acute phase reaction (APR) [18].…”

Section: Introductionmentioning

confidence: 99%

Acute-phase protein concentrations in serum of clinically healthy and diseased European bison (Bison bonasus) – preliminary study

et al. 2022

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Background This is the first report describing levels of APPs in European bison. Serum concentration of acute phase proteins (APPs) may be helpful to assess general health status in wildlife and potentially useful in selecting animals for elimination. Since there is a lack of literature data regarding concentration of APPs in European bisons, establishment of the reference values is also needed. Methods A total of 87 European bison from Polish populations were divided into two groups: (1) healthy: immobilized for transportation, placing a telemetry collar and routine diagnostic purposes; and (2) selectively culled due to the poor health condition. The serum concentration of haptoglobin, serum amyloid A and α1-acid-glycoprotein were determined using commercial quantitative ELISA assays. Since none of the variables met the normality assumptions, non-parametric Mann-Whitney U test was used for all comparisons. Statistical significance was set at p < 0.05. Statistical analyses were performed using Statistica 13.3 (Tibco, USA). Results The concentration of haptoglobin and serum amyloid A was significantly higher in animals culled (euthanised) due to the poor condition in respect to the clinically healthy European bison. The levels of α1-acid-glycoprotein did not show statistical difference between healthy and sick animals. Conclusions Correlation between APPs concertation and health status was proven, therefore the determination of selected APPs may be considered in future as auxiliary predictive tool in assessing European bison health condition.

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Acute phase proteins in three healthy antelope species

Cited by 4 publications

References 14 publications

Protein Electrophoresis and Haptoglobin Values for Captive Bongo (Tragelaphus eurycerus)

Protein Electrophoresis and Haptoglobin Values for Captive Bongo (Tragelaphus eurycerus)

Acute phase reactants in nondomesticated mammals—A veterinary clinical pathology perspective

Acute-phase protein concentrations in serum of clinically healthy and diseased European bison (Bison bonasus) – preliminary study

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