Acute Toxicity Studies of Brucea javanica Merril Leaves Extract on Mice

Angelina, Marissa; Dewijanti, Indah Dwiatmi; Banjarnahor, Sofna; megawati, Megawati; Yuliani, Tri

doi:10.11594/jtls.02.02.01

Cited by 3 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…collected in Indonesia on mice was previously reported [38]. In this investigation, it was reported that vital organs and body average weights of treated leaves ethanol extract did not show no difference compared to control group.…”

Section: Acute Toxicity Of the Aqueous Extract Of B Sumatrana Leavesmentioning

confidence: 57%

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of <i>Brucea sumatrana</i> (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Ehata¹,

Lumpu²,

Munduku³

et al. 2016

View full text Add to dashboard Cite

Results from the in vitro evaluation of the antiparasitaire activity of the aqueous extract, the 80% methanol extract and its fractions from the leaves of Brucea sumatrana against Trypanosoma brucei brucei, T. cruzi, Leishmania infantum, the multidrugresistant K1 and chloroquine-sensitive NF54 strains of Plasmodium falciparum indicated that all samples from the leaves extract presented interesting antiparasitaire activity at different extents. The 80% methanol extract, its chloroform acid, petroleum ether and 80% methanol soluble fractions and the aqueous extract exhibited strong activity against Trypanosoma b. brucei, T. cruzi, L. infantum and the multidrug-resistant K1 strain of P. falciparum with IC 50 values from <0.25 to 4.35 µg/ml as well as against chloroquine-sensitive NF54 strain of P. falciparum with IC 50 values ranging from <0.02 to 2.0.4 µg/ml. Most samples were cytotoxic against MRC-5 cell lines (0.2 < cytotoxic concentration 50(CC 50 ) < 34.24 µg/ml) and showed good selective effect against all tested parasites. In acute toxicity, the aqueous extract was found to be non-toxic and its LD 50 was estimated to be greater than 5 g/kg. In addition, it did not significantly modify the concentration levels of some evaluated biochemical How to cite this paper: Ehata, M.T., Lumpu, S.N., Munduku, C.K., Kabangu, O.K., Cos, P., Maes, L., Apers, S., Vlietinck, A.J., Pieters, L. and Kanyanga, R.C. (2016) 94and hematological parameters in treated rats. These results constitute a scientific validation supporting and justifying the traditional use of the leaves of B. sumatrana for the treatment of malaria, sleeping sickness and at some extent Chagas disease.

show abstract

Section: Acute Toxicity Of the Aqueous Extract Of B Sumatrana Leavesmentioning

confidence: 57%

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of <i>Brucea sumatrana</i> (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Ehata¹,

Lumpu²,

Munduku³

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Other studies also have a similar result, IC 50 of BrD in non-tumorigenic hepatocyte and pancreatic progenitor cells is more than 250 µM [ 41 ] and over 30 µM in normal skin fibroblast [ 42 ]. A study about the acute cytotoxicity of oral administration of Brucea javanica fruit also shows mild cytotoxicity and safe to be used as plant medicine [ 44 ], and administration of high dose BrD (3 mg/kg) in mice was associated with no obvious toxicity [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

Nandana,

Rasyid,

Prihantono

et al. 2024

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Objective: Brucein D (BrD), a quassinoid isolated from Brucea javanica fruit, reportedly demonstrates anti-cancer activity. This study's objective is to evaluate the cytotoxicity of Brucein D and its ability to induce apoptosis in T24 bladder cancer cells. Methods: We investigated the cytotoxic activity of BrD against the T24 cell through the induction of apoptosis in vitro. This cytotoxic activity was evaluated with ΜΤΤ assay and followed by Calcein-AM/PI viability staining. Apoptotic activity was determined with Hoechst 33342 nuclear staining and DNA fragmentation. Doxorubicin and docetaxel were used as a positive control. Evaluation of apoptotic-related gene expression, Bax, Bak, Bcl2, and p53 was also performed using semi-quantitative PCR analysis. Statistical analysis was conducted using One-way ANOVA followed by post hoc test Turkey's HSD (Honestly Significance Difference). Results: Results show that BrD had high toxicity against T24 bladder cancer cells with an IC 50 value of 7.65 ± 1.2 µg/mL but relatively less toxic to 1BR3 normal skin fibroblast cells compared to the doxorubicin and docetaxel treated cells. The viability assay shows that BrD significantly increases the percentage of dead cells relative to control in a dose-dependent manner. Furthermore, the percentage of cells with apoptotic appearance was significantly higher in group treated with BrD IC 50 (56.04±3.09%) compared to control (9.42±2.88). The result was similar to doxorubicin IC 50 (58.97±12.31) but lower than docetaxel IC50 (74.42±9.79). DNA fragmentation in gel electrophoresis was also observed in T24 cells treated with BrD. Apoptosis was also verified by an alteration in the expression of apoptosis-related genes, upregulation of Bax, Bak, and p53, and downregulation of Bcl-2. Conclusion: BrD has shown a cytotoxic effect against T24 bladder cancer cells. Hence, it is a promising natural compound for the management of bladder cancer by induction of apoptosis through activation of the intrinsic pathway, with low toxicity to normal cells.

show abstract

“…Rats treated with P. minima water extract continued to gain weight throughout the 14 days of experiment. According to Angelina et al (2012), the alteration of body weight gain and organ weights of the treatment groups compared to the control group will reflect the toxicity of the substance 19 . In this study, there were no significance differences in body weight gain with control group in the result analysis in p≥0.05.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Acute Oral Toxicity Study of the Water Extract of Physalis Minima Leaves

Ismail¹,

Arsad²,

Lokman³

et al. 2018

J Biol Sci Opin

View full text Add to dashboard Cite

Physalis minima has been used in traditional medicine for the treatment of different ailments. Thus, it is necessary to evaluate the toxicity of this plant's extract to prevent any unwanted side effects. This study aimed to determine the acute oral toxicity level of water extract of P. minima leaves on female Sprague Dawley rats at a dosage of 2000 mg/kg for a period of 14 days using the Organisation for Economic Co-Operation and Development (OECD) Guideline 423. The findings showed no mortality and no change in behavioural pattern. Besides, the body weight of rats increased throughout the study. The rats' internal organs showed a normal histopathological architecture compared to the control group. Hence, it can be concluded that the oral administration of water extract of P. minima did not produce any significant toxic effects on rats.

show abstract

Acute Toxicity Studies of Brucea javanica Merril Leaves Extract on Mice

Cited by 3 publications

References 3 publications

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of <i>Brucea sumatrana</i> (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of <i>Brucea sumatrana</i> (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

Preliminary Acute Oral Toxicity Study of the Water Extract of Physalis Minima Leaves

Contact Info

Product

Resources

About

Acute Toxicity Studies of Brucea javanica Merril Leaves Extract on Mice

Cited by 3 publications

References 3 publications

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of &lt;i&gt;Brucea sumatrana&lt;/i&gt; (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of &lt;i&gt;Brucea sumatrana&lt;/i&gt; (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

Preliminary Acute Oral Toxicity Study of the Water Extract of Physalis Minima Leaves

Contact Info

Product

Resources

About

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of <i>Brucea sumatrana</i> (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of <i>Brucea sumatrana</i> (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo