Adaptation, ancestral variation and gene flow in a ‘Sky Island’ <i>Drosophila</i> species

Hill, Tom; Unckless, Robert L.

doi:10.1111/mec.15701

Cited by 8 publications

(17 citation statements)

References 132 publications

(257 reference statements)

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“…Using a principal component analysis, we find three clear clusters, separating the Chiricahua and Prescott populations, and grouping the Santa Rita and the Huachuca populations together ( fig. 4 ), as seen with the mitochondrial genome and consistent with previous findings ( Jaenike et al 2003 ; Dyer and Jaenike 2005 ; Jaenike and Dyer 2008 ; Hill and Unckless 2020a ).…”

Section: Resultssupporting

confidence: 92%

“…4 B and C ). This signature is not seen in the host, likely due to the recent establishment of D. innubila ( Hill and Unckless 2020a ), particularly in Prescott.…”

Section: Resultsmentioning

confidence: 97%

“…Here, we examined the genome and population genomic variation of w Inn. In a previous survey we collected wild D. innubila from four isolated mountain locations and tested strains for Wolbachia using PCR to amplify the wsp locus and found 48 females infected with Wolbachia ( supplementary table 1 , Supplementary Material online, 13 from the Chiricahua mountains, 27 from Prescott, two from the Huachucas, and six from the Santa Ritas) ( Hill and Unckless 2020a , 2020b ).…”

Section: Resultsmentioning

confidence: 99%

“… Principal component analysis of genetic variation in w Inn. ( A ) Map of locations samples were taken from in this survey, adapted from Hill and Unckless (2020a ). Phoenix and Tucson are shown as points of reference.…”

Section: Resultsmentioning

confidence: 99%

“…We note that while Wolbachia titer is not significantly different as Octomom coverage changes (a proxy for copy number, fig. 5 , Spearman’s ρ = 0.115, GLM t = 1.930, P = 0.0597), it is difficult to infer copy number when sequencing coverage is low (as it is in lower Wolbachia titer individuals) ( Hill and Unckless 2020a ). The lack of resolution precludes any conclusions on their relationship, as it is possible to falsely infer Octomom copy number in some samples.…”

Section: Resultsmentioning

confidence: 99%

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Positive Selection and Horizontal Gene Transfer in the Genome of a Male-Killing Wolbachia

Hill

Unckless

Perlmutter

2021

Molecular Biology and Evolution

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Wolbachia are a genus of widespread bacterial endosymbionts in which some strains can hijack or manipulate arthropod host reproduction. Male killing is one such manipulation in which these maternally transmitted bacteria benefit surviving daughters in part by removing competition with the sons for scarce resources. Despite previous findings of interesting genome features of microbial sex ratio distorters, the population genomics of male-killers remain largely uncharacterized. Here, we uncover several unique features of the genome and population genomics of four Arizonan populations of a male-killing Wolbachia strain, wInn, that infects mushroom-feeding Drosophila innubila. We first compared the wInn genome to other closely related Wolbachia genomes of Drosophila hosts in terms of genome content and confirm that the wInn genome is largely similar in overall gene content to the wMel strain infecting D. melanogaster. However, it also contains many unique genes and repetitive genetic elements that indicate lateral gene transfers between wInn and non-Drosophila eukaryotes. We also find that, in line with literature precedent, genes in the Wolbachia prophage and Octomom regions are under positive selection. Of all the genes under positive selection, many also show evidence of recent horizontal transfer among Wolbachia symbiont genomes. These dynamics of selection and horizontal gene transfer across the genomes of several Wolbachia strains and diverse host species may be important underlying factors in Wolbachia’s success as a male-killer of divergent host species.

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Section: Resultssupporting

confidence: 92%

“…4 B and C ). This signature is not seen in the host, likely due to the recent establishment of D. innubila ( Hill and Unckless 2020a ), particularly in Prescott.…”

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Positive Selection and Horizontal Gene Transfer in the Genome of a Male-Killing Wolbachia

Hill

Unckless

Perlmutter

2021

Molecular Biology and Evolution

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Species distribution models predict genetic isolation of Hetaerina vulnerata Hagen in Selys, 1853 (Odonata, Calopterygidae)

Biddy,

Manthey,

Ware

et al. 2024

Ecology and Evolution

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Understanding how past and current environmental conditions shape the demographic and genetic distributions of organisms facilitates our predictions of how future environmental patterns may affect populations. The Canyon Rubyspot damselfly (Odonata: Zygoptera: Hetaerina vulnerata) is an insect with a range distribution from Colombia to the arid southwestern United States, where it inhabits shaded mountain streams in the arid southwestern United States. Past spatial fragmentation of habitat and limited dispersal capacity of H. vulnerata may cause population isolation and genetic differentiation, and projected climate change may exacerbate isolation by further restricting the species' distribution. We constructed species distribution models (SDMs) based on occurrences of H. vulnerata and environmental variables characterizing the species' niche. We inferred seven current potential population clusters isolated by unsuitable habitat. Paleoclimate models indicated habitat contiguity in past conditions; projected models indicated some habitat fragmentation in future scenarios. Seventy‐eight H. vulnerata individuals from six of the current clusters were sequenced via ddRADseq and processed with Stacks. Principal components and phylogeographic analyses resolved three subpopulations; Structure resolved four subpopulations. FST values were low (<0.05) for nearby populations and >0.15 for populations separated by expanses of unsuitable habitat. Isolation by distance was an existing but weak factor in determining genomic structure; isolation by environment and the intervening landscape explained a significant proportion of genetic distance. Hetaerina vulnerata populations were shown to be isolated by a lack of tree canopy coverage, an important habitat predictor for oviposition and territoriality. Thus, H. vulnerata populations are likely separated and are genetically isolated. Integrating SDMs with landscape genetics allowed us to identify populations separated by distance and unsuitable habitat, explaining population genetic patterns and probable fates for populations under future climate scenarios.

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Diversification of freshwater crabs on the sky islands in the Hengduan Mountains Region, China

Shi,

Pan,

Zhang

et al. 2024

Molecular Phylogenetics and Evolution

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Adaptation, ancestral variation and gene flow in a ‘Sky Island’ Drosophila species

Cited by 8 publications

References 132 publications

Positive Selection and Horizontal Gene Transfer in the Genome of a Male-Killing Wolbachia

Positive Selection and Horizontal Gene Transfer in the Genome of a Male-Killing Wolbachia

Species distribution models predict genetic isolation of Hetaerina vulnerata Hagen in Selys, 1853 (Odonata, Calopterygidae)

Diversification of freshwater crabs on the sky islands in the Hengduan Mountains Region, China

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