Adaptation of adhesion test using Caco-2 cells for anaerobic bacteriumPseudobutyrivibrio xylanivorans, a probiotic candidate

Čepeljnik, Tadej; Lah, B.; Narat, Mojca; Marinšek-Logar, Romana

doi:10.1007/bf02932091

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…Of those, 18 genera showed higher abundance in one rat genotype versus the other. Of the nine genera with higher abundance in BB-DR, three genera, Bifidobacterium, Lactobacillus and Pseudobutyrivibrio , have representatives with known probiotic activity (Silva et al , 1987; Vandenbergh 1993; Jack et al , 1995; Bernet-Camard et al , 1997; Lorca et al , 2001; Reid et al , 2003; Leahy et al , 2005; Ohkawara et al , 2005; Fukuda et al , 2006; Ohkawara et al , 2006; Salminen et al , 2006; Čepeljnik et al , 2003, 2007). These observations from pyrosequencing were verified by qPCR of Bifidobacterium and Lactobacillus .…”

Section: Discussionmentioning

confidence: 99%

Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model

et al. 2009

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Bacteria associated with the onset of type 1 diabetes in a rat model system were identified. In two experiments, stool samples were collected at three time points after birth from bio-breeding diabetes-prone (BB-DP) and bio-breeding diabetes-resistant (BB-DR) rats. DNA was isolated from these samples and the 16S rRNA gene was amplified using universal primer sets. In the first experiment, bands specific to BB-DP and BB-DR genotypes were identified by automated ribosomal intergenic spacer analysis at the time of diabetes onset in BB-DP. Lactobacillus and Bacteroides strains were identified in the BB-DR-and BB-DP-specific bands, respectively. Sanger sequencing showed that the BB-DP and BB-DR bacterial communities differed significantly but too few reads were available to identify significant differences at the genus or species levels. A second experiment confirmed these results using higher throughput pyrosequencing and quantitative PCR of 16S rRNA with more rats per genotype. An average of 4541 and 3381 16S rRNA bacterial reads were obtained from each of the 10 BB-DR and 10 BB-DP samples collected at time of diabetes onset. Nine genera were more abundant in BB-DP whereas another nine genera were more abundant in BB-DR. Thirteen and eleven species were more abundant in BB-DP and BB-DR, respectively. An average of 23% and 10% of all reads could be classified at the genus and species levels, respectively. Quantitative PCR verified the higher abundance of Lactobacillus and Bifidobacterium in the BB-DR samples. Whether these changes are caused by diabetes or are involved in the development of the disease is unknown.

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Section: Discussionmentioning

confidence: 99%

Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model

et al. 2009

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“…However literature overview indicates that although viable probiotics have more documented health effects than nonviable probiotics, the latter are not without effect (Ouwehand and Salminen 1998;Čepeljnik et al 2007). Therefore, we proposed to evaluate the adhesion of fecal lactobacilli to cultured Caco-2 cells.…”

Section: Cfumentioning

confidence: 97%

Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells

et al. 2009

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A quantitative approach has been proposed to evaluate the competitive inhibition of Escherichia coli and Salmonella typhi by live and heat-inactivated laboratory isolated Lactobacillus sp. on adhesion to monolayer of Caco-2 cells. Three species of Lactobacillus (L. casei, L. acidophilus, L. agilis) isolated from human neonate feces and two commercial probiotic strains (L. casei, L. acidophilus) have been compared for probiotic activity. All lactobacilli were able to attach to the Caco-2 cells, however, the degree of adhesion was bacterial strain-dependent. The adhesion indices of the two commercial probiotic strains were not significantly different from the values obtained for the other two similar fecal strains (p > 0.01). The inhibition of attachment of the pathogenic bacteria by inactivated cells of fecal L. acidophilus was examined and compared to the results of live bacteria. The inhibition pattern was similar for live and heat-inactivated L. acidophilus (p > 0.01). The number of attached pathogenic bacteria to the Caco-2 cells decreased when the number of L. acidophilus increased from 10(6) to 10(9) CFU/mL. The heat-inactivated L. acidophilus displayed similar probiotic activity compared to the live bacteria.

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“…) and is commonly used as a model system to study bacterial adhesion (Čepeljnik et al . ; Świątecka et al . ).…”

Section: Discussionmentioning

confidence: 99%

Hydrolysates of glycated and heat-treated peanut 7S globulin (Ara h 1) modulate human gut microbial proliferation, survival and adhesion

et al. 2013

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Aims: Evaluation of an effect of glycation of Ara h 1 on proliferation and survival rate and adhesion of intestinal Enterococcus faecalis, Escherichia coli and Lactobacillus acidophilus. Methods and Results: Pure Ara h 1 heated at three different temperature conditions (G37, G60 and C145°C) in the presence or absence of glucose was subjected to enzymatic hydrolysis. Impacts of Ara h 1 hydrolysates on the bacterial proliferation, survival rate and adhesion to Caco-2 cells in mono and heterogeneous cultures were studied with fluorescent techniques: DAPI, LIVE/ DEAD staining and FISH. Examined hydrolysates hindered proliferation of E. coli and Ent. faecalis with simultaneous decrease in their survival. Maillard reaction (MR, glycation) of Ara h 1 did not alter the effect of hydrolysates on bacterial proliferation rate. Hydrolysates modified at 60 and 145°C with glucose altered the profile of immobilized bacteria, mostly by lowering the number of adhering E. coli and promoting the adhesion of bacteria from genera Lactobacillus and Enterococcus. Conclusions: Ara h1 hydrolysates processed in various ways demonstrated their strong modulatory effect on bacterial proliferation, survival rate and adhesion. Significance and Impact of the Study: Reducing the adhesion of opportunistic bacteria by hydrolysates of Ara h 1 glycated at 60 and 145°C, together with modulation of immobilization of beneficial lactobacilli and enterococci, may be of relevance in terms of the physiological status of the intestinal barrier.

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Adaptation of adhesion test using Caco-2 cells for anaerobic bacteriumPseudobutyrivibrio xylanivorans, a probiotic candidate

Cited by 10 publications

References 25 publications

Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model

Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model

Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells

Hydrolysates of glycated and heat-treated peanut 7S globulin (Ara h 1) modulate human gut microbial proliferation, survival and adhesion

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