Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids

Górecki, Roman Krzysztof; Koryszewska-Bagińska, Anna; Gołębiewski, Marcin; Żylińska, Joanna; Grynberg, Marcin; Bardowski, Jacek

doi:10.1371/journal.pone.0022238

Cited by 40 publications

(64 citation statements)

References 53 publications

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“…lactis SK11, a phage-resistant dairy strain used in cheese making that carries 5 plasmids [7], and L . lactis IL594, the 7-plasmid-containing parent of the plasmid-free laboratory strain IL1403 [8]. The plasmids often specify traits of industrial importance such as the ability to grow on lactose, to utilize milk protein or stress resistance [9,10].…”

Section: Introductionmentioning

confidence: 99%

Plasmid Complement of Lactococcus lactis NCDO712 Reveals a Novel Pilus Gene Cluster

et al. 2016

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Lactococcus lactis MG1363 is an important gram-positive model organism. It is a plasmid-free and phage-cured derivative of strain NCDO712. Plasmid-cured strains facilitate studies on molecular biological aspects, but many properties which make L. lactis an important organism in the dairy industry are plasmid encoded. We sequenced the total DNA of strain NCDO712 and, contrary to earlier reports, revealed that the strain carries 6 rather than 5 plasmids. A new 50-kb plasmid, designated pNZ712, encodes functional nisin immunity (nisCIP) and copper resistance (lcoRSABC). The copper resistance could be used as a marker for the conjugation of pNZ712 to L. lactis MG1614. A genome comparison with the plasmid cured daughter strain MG1363 showed that the number of single nucleotide polymorphisms that accumulated in the laboratory since the strains diverted more than 30 years ago is limited to 11 of which only 5 lead to amino acid changes. The 16-kb plasmid pSH74 was found to contain a novel 8-kb pilus gene cluster spaCB-spaA-srtC1-srtC2, which is predicted to encode a pilin tip protein SpaC, a pilus basal subunit SpaB, and a pilus backbone protein SpaA. The sortases SrtC1/SrtC2 are most likely involved in pilus polymerization while the chromosomally encoded SrtA could act to anchor the pilus to peptidoglycan in the cell wall. Overexpression of the pilus gene cluster from a multi-copy plasmid in L. lactis MG1363 resulted in cell chaining, aggregation, rapid sedimentation and increased conjugation efficiency of the cells. Electron microscopy showed that the over-expression of the pilus gene cluster leads to appendices on the cell surfaces. A deletion of the gene encoding the putative basal protein spaB, by truncating spaCB, led to more pilus-like structures on the cell surface, but cell aggregation and cell chaining were no longer observed. This is consistent with the prediction that spaB is involved in the anchoring of the pili to the cell.

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Section: Introductionmentioning

confidence: 99%

Plasmid Complement of Lactococcus lactis NCDO712 Reveals a Novel Pilus Gene Cluster

et al. 2016

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“…This inter-plasmid competition could result in higher costs for the individual host cell or the entire population [24,25]. Conversely, synergism may occur such that genes encoded on distinct plasmids increase survival or growth of the cell in combination, whereas independently they are less beneficial [26,27].…”

Section: Introductionmentioning

confidence: 99%

Non-additive costs and interactions alter the competitive dynamics of co-occurring ecologically distinct plasmids

et al. 2014

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Plasmids play an important role in shaping bacterial evolution and adaptation to heterogeneous environments. As modular genetic elements that are often conjugative, the selective pressures that act on plasmid-borne genes are distinct from those that act on the chromosome. Many bacteria are coinfected by multiple plasmids that impart niche-specific phenotypes. Thus, in addition to host-plasmid dynamics, interactions between co-infecting plasmids are likely to be important drivers of plasmid population dynamics, evolution and ecology. Agrobacterium tumefaciens is a facultative plant pathogen that commonly harbours two distinct megaplasmids. Virulence depends on the presence of the tumour-inducing (Ti) plasmid, with benefits that are primarily restricted to the disease environment. Here, we demonstrate that a second megaplasmid, the At plasmid, confers a competitive advantage in the rhizosphere. To assess the individual and interactive costs of these plasmids, we generated four isogenic derivatives: plasmidless, pAt only, pTi only and pAtpTi, and performed pairwise competitions under carbonlimiting conditions. These studies reveal a low cost to the virulence plasmid when outside of the disease environment, and a strikingly high cost to the At plasmid. In addition, the costs of pAt and pTi in the same host were significantly lower than predicted based on single plasmid costs, signifying the first demonstration of non-additivity between naturally occurring co-resident plasmids. Based on these empirically demonstrated costs and benefits, we developed a resource-consumer model to generate predictions about the frequencies of these genotypes in relevant environments, showing that non-additivity between co-residing plasmids allows for their stable coexistence across environments.

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“…Both belong to L. lactis subsp. lactis phenotypically, but the parent strain of IL1403 has a citrate permease plasmid (Górecki et al, 2011) and is able to metabolize citrate, placing it with L. lactis subsp. lactis biovar diacetylactis, whereas MG1363 has a lactis phenotype and a cremoris genotype (Kelly et al, 2010).…”

Section: Lactic Acid Bacteriamentioning

confidence: 99%

Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review

Aleksandrzak‐Piekarczyk¹

2013

Lactic Acid Bacteria - R &Amp; D for Food, Health and Livestock Purposes

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Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids

Cited by 40 publications

References 53 publications

Plasmid Complement of Lactococcus lactis NCDO712 Reveals a Novel Pilus Gene Cluster

Plasmid Complement of Lactococcus lactis NCDO712 Reveals a Novel Pilus Gene Cluster

Non-additive costs and interactions alter the competitive dynamics of co-occurring ecologically distinct plasmids

Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review

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