Adaptive evolution to the natural and anthropogenic environment in a global invasive crop pest, the cotton bollworm

Rapid evolution of pesticide resistance via adaptation and interspecific introgression in a major North American crop pest

“…A (0-9Mbp), which shows no signs of admixture, and B (>9Mbp) in which H. armigera haplotypes have introgressed. We next sought to determine whether the admixed samples carried the H. armigera CYP337B3 (Ni et al 2023;Jin et al 2023). Together, these results suggest that two of 237 individuals sampled are admixed, that introgression in these two samples is concentrated around the CYP337B3 locus, and that both samples carry at least one H. armigera CYP337B3 allele.…”

“…In order to conduct intra-and inter-specific comparisons, we used raw sequencing data from multiple Helicoverpa spp. generated by Anderson et al (2018), Taylor et al (2021) and Jin et al (2023). These publicly available data were analysed in the same manner as our newly generated sequence data.…”

“…To reconstruct a tree at the CYP337B3 locus, we used a combination of H. zea samples collected here (two admixed and two representative non-admixed samples), 4 H. zea collected in 2002 by , 2 H. punctigera and 9 H. armigera collected from both the invasive range in Brazil by Anderson et al (2018) (which are admixed with H. zea), and 10 H. armigera from the major clades of the native range by Jin et al (2023) (see Supplementary Table S2). These samples were mapped, jointly genotyped and filtered without phasing in the manner as described above.…”

Genome-Wide Scanning Loci and Differentially Expressed Gene Analysis Unveils the Molecular Mechanism of Chlorantraniliprole Resistance in Spodoptera frugiperda

“…evolution of cold tolerance. 50 The tissue distribution results of high expression of Sf Tret1 in the fat body and hemolymph also suggest that this may be involved in trehalose transport in S. frugiperda (Figure 6B). Through RNAi combined with trehalose content determination, we speculated that the Ch-R strain could increase the trehalose content in the cell of S. frugiperda by upregulating the expression of Sf Tret1, thereby indirectly improving the tolerance to chlorantraniliprole (Figure 6C−G).…”

“…The trehalose transporter can transport trehalose from the fat body to the hemolymph to maintain trehalose balance in insects . Recent research has identified a mutation in a Tret gene in cotton bollworm that enables the evolution of cold tolerance . The tissue distribution results of high expression of SfTret1 in the fat body and hemolymph also suggest that this may be involved in trehalose transport in S.…”