Adaptive laboratory evolution to hypersaline conditions of lactic acid bacteria isolated from seaweed

Papadopoulou, Eleftheria; Evgrafov, Mari Cristina Rodriguez de; Kalea, Argyro; Tsapekos, Panagiotis; Angelidaki, Irini

doi:10.1016/j.nbt.2023.03.001

Cited by 9 publications

(3 citation statements)

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“…Although in some studies, the substitution of NaCl with KCl had no significant impact on the growth of LAB (Ayyash and Shah, 2010;Kamleh et al, 2012;Juan et al, 2022), others showed that this change promotes their growth (Karimi et al, 2012). However, differences at the strain level could lead to this discrepancy which raises questions about how the effect of salts varies among LAB strains in terms of growth and metabolic response, for instance, their acidification ability (Papadopoulou et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

High-throughput characterization of the effect of sodium chloride and potassium chloride on 31 lactic acid bacteria and their co-cultures

Ndiaye,

Fliss,

Filteau

2024

Front. Microbiol.

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Salt (NaCl) is associated with a risk of hypertension and the development of coronary heart disease, so its consumption should be limited. However, salt plays a key role in the quality and safety of food by controlling undesirable microorganisms. Since studies have focused primarily on the effect of salts on the overall counts of the lactic acid bacteria (LAB) group, we have not yet understood how salt stress individually affects the strains and the interactions between them. In this study, we characterized the effect of sodium chloride (NaCl) and potassium chloride (KCl) on the growth and acidification of 31 LAB strains. In addition, we evaluated the effect of salts on a total of 93 random pairwise strain combinations. Strains and co-cultures were tested at 3% NaCl, 5% NaCl, and 3% KCl on solid medium using an automated approach and image analysis. The results showed that the growth of LAB was significantly reduced by up to 68% at 5% NaCl (p < 0.0001). For the co-cultures, a reduction of up to 57% was observed at 5% NaCl (p < 0.0001). However, acidification was less affected by salt stress, whether for monocultures or co-cultures. Furthermore, KCl had a lesser impact on both growth and acidification compared to NaCl. Indeed, some strains showed a significant increase in growth at 3% KCl, such as Lactococcus lactis subsp. lactis 74310 (23%, p = 0.01). More importantly, co-cultures appeared to be more resilient and had more varied responses to salt stress than the monocultures, as several cases of suppression of the significant effect of salts on acidification and growth were detected. Our results highlight that while salts can modulate microbial interactions, these latter can also attenuate the effect of salts on LAB.

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Section: Introductionmentioning

confidence: 99%

High-throughput characterization of the effect of sodium chloride and potassium chloride on 31 lactic acid bacteria and their co-cultures

Ndiaye,

Fliss,

Filteau

2024

Front. Microbiol.

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“…As a mature and effective technique, ALE has been proven highly effective in the optimization of production strains [12]. For example, the strains of Lactobacillus plantarum and Enterococcus faecium, which are producers of seaweed biomass, demonstrated a respective increase of 1.29-fold and 1.75fold in their tolerance to salt, evolved by continuously increasing the gradient of NaCl concentration during cultivation (35,50,71,100,141, and 200 g/L) [13]. Salttolerant Chlamydomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Adaptive laboratory evolution of Rhodococcus rhodochrous DSM6263 for chlorophenol degradation under hypersaline condition

Zheng,

Zhang,

et al. 2023

Microb Cell Fact

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Background Normally, a salt amount greater than 3.5% (w/v) is defined as hypersaline. Large amounts of hypersaline wastewater containing organic pollutants need to be treated before it can be discharged into the environment. The most critical aspect of the biological treatment of saline wastewater is the inhibitory/toxic effect exerted on bacterial metabolism by high salt concentrations. Although efforts have been dedicated to improving the performance through the use of salt-tolerant or halophilic bacteria, the diversities of the strains and the range of substrate spectrum remain limited, especially in chlorophenol wastewater treatment. Results In this study, a salt-tolerant chlorophenol-degrading strain was generated from Rhodococcus rhodochrous DSM6263, an original aniline degrader, by adaptive laboratory evolution. The evolved strain R. rhodochrous CP-8 could tolerant 8% NaCl with 4-chlorophenol degradation capacity. The synonymous mutation in phosphodiesterase of strain CP-8 may retard the hydrolysis of cyclic adenosine monophosphate (cAMP), which is a key factor reported in the osmoregulation. The experimentally verified up-regulation of intracellular cAMP level in the evolved strain CP-8 contributes to the improvement of growth phenotype under high osmotic condition. Additionally, a point mutant of the catechol 1,2-dioxygenase, CatAN211S, was revealed to show the 1.9-fold increment on activity, which the mechanism was well explained by molecular docking analysis. Conclusions This study developed one chlorophenol-degrading strain with extraordinary capacity of salt tolerance, which showed great application potential in hypersaline chlorophenol wastewater treatment. The synonymous mutation in phosphodiesterase resulted in the change of intracellular cAMP concentration and then increase the osmotic tolerance in the evolved strain. The catechol 1,2-dioxygenase mutant with improved activity also facilitated chlorophenol removal since it is the key enzyme in the degradation pathway.

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“…2023b).While effective,the ion exchange method has limitations in terms of exchange capacity. The membrane separation method is a technology that leverages the pore size of the membrane and electrostatic effects.This allows for the selective permeability of different ions under external force,effectively separating phosphorus from solvents in wastewater (Papadopoulou et al, 2023).The operation of membrane technology for phosphorus removal is often constrained by both the characteristics of the membrane and the characteristics of the wastewater.…”

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confidence: 99%

A review：Modified biochar as an adsorbent for phosphorus removal from water

Tian,

Huang,

Zhang

et al. 2024

int.jour.sci.res.mana

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In recent years,eutrophication of water bodies has become increasingly severe due to the rising content of phosphorus in these bodies.This escalation adversely affects the normal growth of aquatic organisms,leading to the deterioration of water quality,and ultimately impacting human life.Phosphorus removal is a crucial direction for addressing water body pollution.Therefore,it is of utmost importance to identify efficient,affordable,and feasible methods for removing phosphorus from wastewater.Adsorption,owing to its simplicity,efficiency,and low cost,is widely employed in water treatment.Biochar,in particular,is extensively used due to its high efficiency,renewability,low cost,and the ready availability of raw materials.This paper reviews the preparation methods of biochar and modified biochar,analyses the effects of pyrolysis temperature,solution pH,reaction temperature,adsorbent dosage,adsorption time and co-existing ions on the phosphorus removal of biochar materials.It also explores the necessity of desorption and regeneration of biochar,along with the prospects of biochar in the future of phosphorus removal.

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Adaptive laboratory evolution to hypersaline conditions of lactic acid bacteria isolated from seaweed

Cited by 9 publications

References 50 publications

High-throughput characterization of the effect of sodium chloride and potassium chloride on 31 lactic acid bacteria and their co-cultures

High-throughput characterization of the effect of sodium chloride and potassium chloride on 31 lactic acid bacteria and their co-cultures

Adaptive laboratory evolution of Rhodococcus rhodochrous DSM6263 for chlorophenol degradation under hypersaline condition

A review：Modified biochar as an adsorbent for phosphorus removal from water

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