Adaptive-Resolution Multi-Orientation Analysis of Complex Filamentous Network Images

Abstract

13 14 Microscopy images of cytoskeletal, 15 nucleoskeletal, and other filamentous 16 structures contain complex junctions where 17 multiple filaments with distinct orientations 18overlap, yet state-of-the-art software generally 19uses single orientation analysis to segment 20 these structures. We describe an image 21 analysis approach to simultaneously segment 22both filamentous structures and their 23 intersections in microscopy images, based on 24 analytically resolving coincident multiple 25 orientations upon image filtering in a manner 26 that balances orientation resolution and 27 spatial localization. 28