Adaptive response in irradiated human lymphocytes: radiobiological and genetical aspects

Khandogina, E.K.; Mutovin, G.R.; Zvereva, S.V.; Antipov, A.V.; Zverev, D.O.; Akifyev, A. P.

doi:10.1016/0027-5107(91)90073-w

Cited by 22 publications

(6 citation statements)

References 10 publications

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“…Shadley et al [11] and others [12,13] have pointed out that resting human lymphocytes cannot be triggered to induce an adaptive response under in vitro conditions. However, Sanderson and Morley [14], Cai and Liu [15] as well as Khandogina et al [16] did find a positive response in G 0 lymphocytes. Thus, the universal existence of RAR still remains a matter of debate and dispute.…”

Section: Introductionmentioning

confidence: 92%

Influence of in vitro low-level gamma-radiation on the UV-induced DNA repair capacity of human lymphocytes - analysed by unscheduled DNA synthesis (UDS) and comet assay

Mohankumar

Paul

Prakash

et al. 1998

Radiation and Environmental Biophysics

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Unscheduled DNA synthesis (UDS) induced by ultraviolet radiation (UV) was studied in human lymphocytes after exposing blood samples in vitro to doses ranging between 1 and 10 mGy gamma-radiation, by way of measuring tritiated thymidine (3H-TdR) uptake in the DNA of these lymphocytes. The results indicate that samples pre-exposed to gamma-ray doses ranging between 2.5 and 4 mGy show higher UDS levels compared with those pre-exposed to doses of less than 2.5 or more than 4 mGy. These results were verified by studying the rate of removal of UV-induced photoproducts using the comet assay. The reason for the increase in DNA repair capacity in this dose range is discussed in comparison with earlier reports on this phenomenon. The DNA repair capacity with respect to inter-individual variability and age is also analysed. The study implies that the comet assay is a simple and sensitive visual method to track nucleotide excision repair and hence can be used to estimate UV-induced DNA repair in the place of the more reliable yet cumbersome and time-consuming, grain-counting autoradiographic technique.

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Section: Introductionmentioning

confidence: 92%

Influence of in vitro low-level gamma-radiation on the UV-induced DNA repair capacity of human lymphocytes - analysed by unscheduled DNA synthesis (UDS) and comet assay

Mohankumar

Paul

Prakash

et al. 1998

Radiation and Environmental Biophysics

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“…On the other hand, when chromosomal aberrations were analyzed, both positive [35] and negative adaptive responses [36] were reported. The reasons for the conflicting results are not understood.…”

Section: Discussionmentioning

confidence: 97%

Studies of adaptive response and mutation induction in MCF-10A cells following exposure to chronic or acute ionizing radiation

Manesh

Sangsuwan

Wójcik

et al. 2015

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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“…Shadley et al (1987) were the first to claim that the adaptive response cannot be triggered in G o human lymphocytes, and this observation was later confirmed by Moquet et al (1989) and Wang et al (1991), all working under in vitro conditions. On the other hand, Sanderson and Morley (1986), Cai and Liu (1990) and Khandogina et al (1991) did find an adaptive response in G o lymphocytes irradiated in vitro. Furthermore, Tuschl et al (1980Tuschl et al ( , 1983 described an enhanced UV-light-induced unscheduled DNA synthesis (UDS) frequency and reduced mitomycin-C-induced sister chromatid exchanges in peripheral lymphocytes of persons occupationally exposed to low levels of ionizing radiation.…”

Section: Introductionmentioning

confidence: 87%

No indications of an enhanced UV-light-induced unscheduled DNA synthesis in splenocytes of mice following a low-dose irradiation in vivo or in vitro

Wójcik

Seemayer

Müller

et al. 1995

Radiat Environ Biophys

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One of the open questions regarding the adaptive response to ionizing radiation is whether it can be induced in G0 lymphocytes. In the majority of experiments in which an adaptive response in G0 lymphocytes was observed, the adapting dose was applied in vivo. In order to investigate whether there is some in vivo component of adaptive response, mouse splenocytes of the C57BL/6 strain were irradiated with 0.1 Gy x-rays either in vivo or in vitro, and their UV-light-induced unscheduled DNA synthesis (UDS) levels were determined autoradiographically. An augmented UV-light-induced UDS following an adapting dose applied in vivo has previously been described by several authors in splenocytes of C57BL/6 mice, indicating that the adapting dose enhanced the DNA repair capacity of lymphocytes. In the present investigation, however, no evidence of an adaptive response could be seen regardless of whether the adapting dose was given in vivo or in vitro. Those results present a further indication for the fact that the adaptive response to ionizing radiation is not always inducible, even in lymphocytes of an inbred mouse strain in which its existence has been reported before.

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Adaptive response in irradiated human lymphocytes: radiobiological and genetical aspects

Cited by 22 publications

References 10 publications

Influence of in vitro low-level gamma-radiation on the UV-induced DNA repair capacity of human lymphocytes - analysed by unscheduled DNA synthesis (UDS) and comet assay

Influence of in vitro low-level gamma-radiation on the UV-induced DNA repair capacity of human lymphocytes - analysed by unscheduled DNA synthesis (UDS) and comet assay

Studies of adaptive response and mutation induction in MCF-10A cells following exposure to chronic or acute ionizing radiation

No indications of an enhanced UV-light-induced unscheduled DNA synthesis in splenocytes of mice following a low-dose irradiation in vivo or in vitro

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