Additives migrating from 3D-printed plastic induce developmental toxicity and neuro-behavioural alterations in early life zebrafish (Danio rerio)

Walpitagama, Milanga; Carve, Megan; Douek, Alon M.; Trestrail, Charlene; Bai, Yutao; Kaslin, Jan; Wlodkowic, Donald

doi:10.1016/j.aquatox.2019.105227

Cited by 54 publications

(27 citation statements)

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“…Despite these advances, an effective treatment paradigm remains elusive, partially due to the phenotypic complexity of MPS IIIA, as well as the still indeterminate mechanistic explanation of the link between the aetiology of the disease (i.e., impaired HS catabolism) and the manifestation of severe, progressive CNS dysfunction. As a model organism, the zebrafish has been used for diverse purposes ranging from ecotoxicology [ 67 ], to evolutionary [ 68 ] and developmental biology [ 69 ], to myriad uses in the biomedical sciences including regeneration of the CNS [ 70 , 71 ] and skeletal muscle following profound traumatic injury [ 72 ]. Most pertinent to this study, however, is the utility of the zebrafish as a model for inherited genetic disease [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

An Engineered sgsh Mutant Zebrafish Recapitulates Molecular and Behavioural Pathobiology of Sanfilippo Syndrome A/MPS IIIA

Douek

Khabooshan

Henry

et al. 2021

IJMS

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Mucopolysaccharidosis IIIA (MPS IIIA, Sanfilippo syndrome type A), a paediatric neurological lysosomal storage disease, is caused by impaired function of the enzyme N-sulfoglucosamine sulfohydrolase (SGSH) resulting in impaired catabolism of heparan sulfate glycosaminoglycan (HS GAG) and its accumulation in tissues. MPS IIIA represents a significant proportion of childhood dementias. This condition generally leads to patient death in the teenage years, yet no effective therapy exists for MPS IIIA and a complete understanding of the mechanisms of MPS IIIA pathogenesis is lacking. Here, we employ targeted CRISPR/Cas9 mutagenesis to generate a model of MPS IIIA in the zebrafish, a model organism with strong genetic tractability and amenity for high-throughput screening. The sgshΔex5−6 zebrafish mutant exhibits a complete absence of Sgsh enzymatic activity, leading to progressive accumulation of HS degradation products with age. sgshΔex5−6 zebrafish faithfully recapitulate diverse CNS-specific features of MPS IIIA, including neuronal lysosomal overabundance, complex behavioural phenotypes, and profound, lifelong neuroinflammation. We further demonstrate that neuroinflammation in sgshΔex5−6 zebrafish is largely dependent on interleukin-1β and can be attenuated via the pharmacological inhibition of Caspase-1, which partially rescues behavioural abnormalities in sgshΔex5−6 mutant larvae in a context-dependent manner. We expect the sgshΔex5−6 zebrafish mutant to be a valuable resource in gaining a better understanding of MPS IIIA pathobiology towards the development of timely and effective therapeutic interventions.

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Section: Discussionmentioning

confidence: 99%

An Engineered sgsh Mutant Zebrafish Recapitulates Molecular and Behavioural Pathobiology of Sanfilippo Syndrome A/MPS IIIA

Douek

Khabooshan

Henry

et al. 2021

IJMS

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“…Athey et al, 2020; LaPlaca & van den Hurk, 2020; Lei et al, 2018; Xia et al, 2020). Functional disruption of key organs, such as the kidneys, liver and brain, can then impact body fluid composition, neurotransmitter and endocrine pathways (Walpitagama et al, 2019; Wang, Wei, et al, 2019), with survival consequences for the individual (Figure 2).…”

Section: Impacts Of Ingested Mps On Freshwater Fishesmentioning

confidence: 99%

Microplastics in freshwater fishes: Occurrence, impacts and future perspectives

et al. 2021

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Microplastics (MPs) are small, plastic particles of various shapes, sizes and polymers.Although well studied in marine systems, their roles and importance in freshwater environments remain uncertain. Nevertheless, the restricted ranges and variable traits of freshwater fishes result in their communities being important receptors and strong bioindicators of MP pollution. Here, the current knowledge on MPs in freshwater fishes is synthesized, along with the development of recommendations for future research and sample processing. MPs are commonly ingested and passively taken up by numerous freshwater fishes, with ingestion patterns often related to individual traits (e.g. body size, trophic level) and environmental factors (e.g. local urbanization, habitat features). Controlled MP exposure studies highlight various effects on fish physiology, biochemistry and behaviour that are often complex, unpredictable, species-specific and nonlinear in respect of dose-response relationships.Egestion is typically rapid and effective, although particles of a particular shape and/ or size may remain, or translocate across the intestinal wall to other organs via the blood. Regarding future studies, there is a need to understand the interactions of MP pollution with other anthropogenic stressors (e.g. warming, eutrophication), with a concomitant requirement to increase the complexity of studies to enable impact assessment at population, community and ecosystem levels, and to determine whether there are consequences for processes, such as parasite transmission, where MPs could vector parasites or increase infection susceptibility. This knowledge will determine the extent to which MP pollution can be considered a major anthropogenic stressor of freshwaters in this era of global environmental change.

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“…This type of essay allows us to elucidate the mechanism of action of many substances. Many procedures to perform the biochemical assays developed for other species ( Ellman et al, 1961 ; Misra and Fridovich, 1972 ; Bradford, 1976 ; Aebi, 1984 ; Draper and Hadley, 1990 ; LeBel et al, 1992 ; Frasco and Guilhermino, 2002 ) has been adapted and used in zebrafish with great results ( Jin et al, 2015 ; Abe et al, 2018 ; Agostini et al, 2018 ; Maharajan et al, 2018 ; Sarasamma et al, 2018 , 2019 ; Bernardo et al, 2019 ; Mocelin et al, 2019 ; Schmitt et al, 2019 ; Ünal et al, 2019 ; Walpitagama et al, 2019 ).…”

Section: Evaluation Of Sars-cov-2 Neurologic Alterations Through Zebrafish’s Behaviormentioning

confidence: 99%

“…For instance, some tests allow the determination of the reactive oxygen species, the lipid peroxidation, and the activity of the enzymes involved in antioxidant mechanisms, such as superoxide dismutase, catalase, and glutathione-S-transferase ( Diniz et al, 2015 ; Jin et al, 2015 ; Sarasamma et al, 2018 ). Besides, some assays enable the measurement of distinct neurotransmitters activity, such as the choline, GABA, glutamate, glycine, dopamine, and 5-hydroxytryptamine ( Abe et al, 2018 ; Agostini et al, 2018 ; Sarasamma et al, 2018 , 2019 ; Schmitt et al, 2019 ; Walpitagama et al, 2019 ).…”

Section: Evaluation Of Sars-cov-2 Neurologic Alterations Through Zebrafish’s Behaviormentioning

confidence: 99%

“…This type of essay allows us to elucidate the mechanism of action of many substances. Many procedures to perform the biochemical assays developed for other species (Aebi, 1984;Bradford, 1976;Draper and Hadley, 1990;Ellman et al, 1961;Frasco and Guilhermino, 2002;LeBel et al, 1992;Misra and Fridovich, 1972) has been adapted and used in zebrafish with great results (Abe et al, 2018;Agostini et al, 2018;Bernardo et al, 2019;Jin et al, 2015;Maharajan et al, 2018;Mocelin et al, 2019;Sarasamma et al, 2019Sarasamma et al, , 2018Schmitt et al, 2019;Ünal et al, 2019;Walpitagama et al, 2019).…”

Section: Molecular and Biochemical Analysis In The Zebrafish Modelmentioning

confidence: 99%

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Zebrafish as a Translational Model: An Experimental Alternative to Study the Mechanisms Involved in Anosmia and Possible Neurodegenerative Aspects of COVID-19?

Costa

Brigante

Fernandes

et al. 2021

eNeuro

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Additives migrating from 3D-printed plastic induce developmental toxicity and neuro-behavioural alterations in early life zebrafish (Danio rerio)

Cited by 54 publications

References 55 publications

An Engineered sgsh Mutant Zebrafish Recapitulates Molecular and Behavioural Pathobiology of Sanfilippo Syndrome A/MPS IIIA

An Engineered sgsh Mutant Zebrafish Recapitulates Molecular and Behavioural Pathobiology of Sanfilippo Syndrome A/MPS IIIA

Microplastics in freshwater fishes: Occurrence, impacts and future perspectives

Zebrafish as a Translational Model: An Experimental Alternative to Study the Mechanisms Involved in Anosmia and Possible Neurodegenerative Aspects of COVID-19?

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