Address: W. M. Kozlowski, Posen

Kozƚowski, Wojciech M.

doi:10.5840/wcp61927121

Cited by 21 publications

(51 citation statements)

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“…The Cu II ione xhibits high affinity towards nitrogen donors, and is one of very few metal ions able to deprotonate and coordinate the peptide bond nitrogen. [4][5][6][7] Moreover,b inding with the N d of the His side chain creates as table 6-membered chelate ring with the precedinga mide nitrogen. Such rings are also achievable through Asp and Cys residues.A sp is ah ard acid, and therefore it binds Cu II weaker than His.…”

Section: Identity Of the Amino Acidmentioning

confidence: 99%

“…Thus, the Cu II -binding becomes much weaker. [7] His at position 1p rovides as His-1 peptidem olecule, resulting in a2:1 4N complex (2 NH 2 ; 2 Im), able to provide high affinity binding only at ahighconcentration.A tl ower concentrations andr atios of about 1:1, XH and XZH motifs bind Cu II much stronger.N otably,H is analogues [(4-thiazolyl)-l-alanine, (2-pyridyl)-l-alanine, and (pyrazol-1-yl)-l-alanine] can also form stable 4N motifs with affinities similart oHis. [7]…”

Section: Influence Of His Positiononm Etal Bindingmentioning

confidence: 99%

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N‐Terminal Cu‐Binding Motifs (Xxx‐Zzz‐His, Xxx‐His) and Their Derivatives: Chemistry, Biology and Medicinal Applications

González

Bossak

Stefaniak

et al. 2018

Chemistry A European J

117

203

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Peptides and proteins with N-terminal amino acid sequences NH -Xxx-His (XH) and NH -Xxx-Zzz-His (XZH) form well-established high-affinity Cu -complexes. Key examples are Asp-Ala-His (in serum albumin) and Gly-His-Lys, the wound healing factor. This opens a straightforward way to add a high-affinity Cu -binding site to almost any peptide or protein, by chemical or recombinant approaches. Thus, these motifs, NH -Xxx-Zzz-His in particular, have been used to equip peptides and proteins with a multitude of functions based on the redox activity of Cu, including nuclease, protease, glycosidase, or oxygen activation properties, useful in anticancer or antimicrobial drugs. More recent research suggests novel biological functions, mainly based on the redox inertness of Cu in XZH, like PET imaging (with Cu), chelation therapies (for instance in Alzheimer's disease and other types of neurodegeneration), antioxidant units, Cu transporters and activation of biological functions by strong Cu binding. This Review gives an overview of the chemical properties of Cu-XH and -XZH motifs and discusses the pros and cons of the vastly different biological applications, and how they could be improved depending on the application.

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Section: Identity Of the Amino Acidmentioning

confidence: 99%

Section: Influence Of His Positiononm Etal Bindingmentioning

confidence: 99%

N‐Terminal Cu‐Binding Motifs (Xxx‐Zzz‐His, Xxx‐His) and Their Derivatives: Chemistry, Biology and Medicinal Applications

González

Bossak

Stefaniak

et al. 2018

Chemistry A European J

117

203

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“…Dziś nie potrafimy przypomnieć sobie nawet tytułów przeczytanych w ten sposób książek. że takie czytanie nie wiele wzbogaca umysł, tego nie potrzebujemy tłumaczyć" 20 .…”

Section: Rozwaga Umiar Refleksja W Czytaniuunclassified

“…Przekonywał o tym w swoim poradniku W. Kozłowski, twierdząc dodatkowo, iż: "przeglądając co pewien czas swoje notatki, czytelnik systematyczny może zdać sobie sprawę z postępu swego wykształcenia" 33 . W przypadku czytania literatury pięknej wskazywano też ogromną wartość spisywania złotych myśli (J. Skarżyńska, I. Moszczeń-ska, J. Ippoldt, J. Paszenda).…”

Section: Prowadzenie Dzienniczka Lekturunclassified

“…Podobnie entuzjastycznie, wskazując jego zalety czytelnicze, wypowiadał się W. M. Kozłowski: "Prowadzenie dziennika lektury nie tylko jest najlepszym środkiem skupienia się nad przeczytaną książką oraz wydobycia z siebie samodzielnej i wszechstronnej jej oceny; osiągamy z niego inne jeszcze korzyści. Jedną z nich jest łatwość uprzytomnienia sobie treści przeczytanego po upływie pewnego czasu" 36 . Autor podawał przy tym dokładną instrukcję zawartości dziennika lektury: "W dzienniku takim notować należy możliwie treściwie zawarte w książce myśli lub wiadomości, jako też te myśli lub uwagi, które się nasuwają przy jej czytaniu, a wreszcie i samą ocenę dzieła (...)" 37 .…”

Section: Prowadzenie Dzienniczka Lekturunclassified

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Recommendations for reading fiction in Polish reading guides from the period between World War I and World War II

Nosek¹

2012

Linguodidactica

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"Tymczasem trzeba i umieć czytać i lubić.Przychodzi się do tego nie od razu i nie łatwo" The article describes a number of recommendations for the reading and reception of belletristic books in Polish reading guides from the period between World War I and World War II. The authors of the books from this period encouraged readers to choose only valuable books, to read systhematically but in moderation, to develop a critical approach to reading litarature, to internalise the content of the reading material, to participate in discussions about books and to keep a record of the books they have read in order to raise the effectiveness of reading fiction.

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Copper, BDNF and Its N‐terminal Domain: Inorganic Features and Biological Perspectives

Travaglia

Mendola

Magrı̀

et al. 2012

Chemistry A European J

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Brain-derived neurotrophic factor (BDNF) is a neurotrophin that influences development, maintenance, survival, and synaptic plasticity of central and peripheral nervous systems. Altered BDNF signaling is involved in several neurodegenerative disorders including Alzheimer's disease. Metal ions may influence the BDNF activity and it is well known that the alteration of Cu(2+) homeostasis is a prominent factor in the development of neurological pathologies. The N-terminal domain of BDNF represents the recognition site of its specific receptor TrkB, and metal ions interaction with this protein domain may influence the protein/receptor interaction. In spite of this, no data inherent the interaction of BDNF with Cu(2+) ions has been reported up to now. Cu(2+) complexes of the peptide fragment BDNF(1-12) encompassing the sequence 1-12 of N-terminal domain of human BDNF protein were characterized by means of potentiometry, spectroscopic methods (UV/Vis, CD, EPR), parallel tempering simulations and DFT-geometry optimizations. Coordination features of the acetylated form, Ac-BDNF(1-12), were also characterized to understand the involvement of the terminal amino group. Whereas, an analogous peptide, BDNF(1-12)D3N, in which the aspartate residue was substituted by an asparagine, was synthesized to provide evidence on the possible role of carboxylate group in Cu(2+) coordination. The results demonstrated that the amino group is involved in metal binding and the metal coordination environment of the predominant complex species at physiological pH consisted of one amino group, two amide nitrogen atoms, and one carboxylate group. Noteworthy, a strong decrease of the proliferative activity of both BDNF(1-12) and the whole protein on a SHSY5Y neuroblastoma cell line was found after treatment in the presence of Cu(2+). The effect of metal addition is opposite to that observed for the analogous fragment of nerve growth factor (NGF) protein, highlighting the role of specific domains, and suggesting that Cu(2+) may drive different pathways for the BDNF and NGF in physiological as well as pathological conditions.

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Address: W. M. Kozlowski, Posen

Cited by 21 publications

References 0 publications

N‐Terminal Cu‐Binding Motifs (Xxx‐Zzz‐His, Xxx‐His) and Their Derivatives: Chemistry, Biology and Medicinal Applications

N‐Terminal Cu‐Binding Motifs (Xxx‐Zzz‐His, Xxx‐His) and Their Derivatives: Chemistry, Biology and Medicinal Applications

Recommendations for reading fiction in Polish reading guides from the period between World War I and World War II

Copper, BDNF and Its N‐terminal Domain: Inorganic Features and Biological Perspectives

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