Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm

Frolikova, Michaela; Valaskova, Eliska; Černý, Jiří; Lumeau, Audrey; Šebková, Nataša; Palenikova, Veronika; Sanches-Hernandez, Noemi; Pohlová, Alžběta; Maňásková-Postlerová, Pavla; Dvořáková-Hortová, Kateřina

doi:10.3390/ijms20051004

Cited by 14 publications

(24 citation statements)

References 45 publications

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“…In our previous study, we showed a presence of both α6β1 and α6β4 integrin heterodimers on sperm 20 . However, the α6 subunit preferentially creates the α6β4 heterodimer in the presence of both β4 and β1 subunits 23,51,52 .…”

Section: Discussionmentioning

confidence: 80%

“…With the ability of tetraspanins being part of signalling cascades involving cytoskeleton 5,15,16 they also play a crucial role in the organization of functional membrane protein cluster domains called a tetraspanin web, which were also described in oocyte and recently in sperm. The integral part of the tetraspanin web are integrins and the presence of α3β1, α6β1 and α6β4 integrins was shown in sperm [17][18][19][20] . Importantly, in somatic cells, tetraspanin CD151 was found to play a key role in regulating the adhesion strengthening of integrin α6β1 21 .…”

mentioning

confidence: 99%

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Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Jankovičová

Frolikova

Palenikova

et al. 2020

Sci Rep

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The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a newly discovered presence of CD151 in sperm, and present its expression and distribution during spermatogenesis and sperm transition during the acrosome reaction. We traced CD151 gene and protein expression in testicular cell subpopulations, with strong enrichment in spermatogonia and spermatids. The testicular and epididymal localization pattern is designated to the sperm head primary fusion site called the equatorial segment and when compared to the acrosome vesicle status, CD151 was located into the inner acrosomal membrane overlying the nucleus. Moreover, we show CD151 interaction with α6 integrin subunit, which forms a dimer with β4 as a part of cisprotein interactions within sperm prior to gamete fusion. We used mammalian species with distinct sperm morphology and sperm maturation such as mouse and bull and compared the results with human. In conclusion, the delivered findings characterise CD151 as a novel sperm tetraspanin network member and provide knowledge on its physiology in male germ cells.CD151 belongs to the tetraspanin superfamily, these proteins participate in many biological processes such as growth control, intracellular signalling, cell adhesion, migration, motility 1-4 and they also take place in pathogenesis of some human diseases 5,6 . Tetraspanins create a scaffolding multiprotein network in the membrane that anchors other proteins into specific domains with precise functions and moreover, they are indispensable players in the mammalian fertilization process. Tetraspanin CD9 expressed on mouse eggs is considered as an essential molecule for successful gamete fusion 7-9 as well as tetraspanin CD81 which is also involved in fertilization 10,11 . Our previous studies concerning species-specific traits of CD9 and CD81 in mouse, bull and porcine gametes during their functional maturation and fertilization, suggested the active participation of tetraspanins in these events [11][12][13][14] . With the ability of tetraspanins being part of signalling cascades involving cytoskeleton 5,15,16 they also play a crucial role in the organization of functional membrane protein cluster domains called a tetraspanin web, which were also described in oocyte and recently in sperm. The integral part of the tetraspanin web are integrins and the presence of α3β1, α6β1 and α6β4 integrins was shown in sperm [17][18][19][20] . Importantly, in somatic cells, tetraspanin CD151 was found to play a key role in regulating the adhesion strengthening of integrin α6β1 21 . CD151 has previously been linked with human gamete fusion 22 , associates specifically with α3, α6 and α7 integrins 1,3,23 and links α3β1 and α6β1 integrins to other tetraspanins, including CD9 [24][25][26][27] . Based on that knowledge we p...

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Section: Discussionmentioning

confidence: 80%

mentioning

confidence: 99%

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Jankovičová

Frolikova

Palenikova

et al. 2020

Sci Rep

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“…The adhesion molecule trophinin was shown to be associated with sperm motility in humans (Hatakeyama et al, 2008) and mice (Park et al, 2012). Once sperm arrive at the oviducts, adhesion molecules facilitate the attachment of sperm to the oviductal epithelium (Aviles, Gutierrez‐Adan, & Coy, 2010; Frolikova et al, 2016, 2019; Talevi & Gualtieri, 2010). This is an important step that prevents premature capacitation in sperm.…”

Section: Adhesion Molecules and Reproductionmentioning

confidence: 99%

“…Once sperm arrive at the oviducts, adhesion molecules facilitate the attachment of sperm to the oviductal epithelium (Aviles, Gutierrez-Adan, & Coy, 2010;Frolikova et al, 2016Frolikova et al, , 2019Talevi & Gualtieri, 2010). This is an important step that prevents premature capacitation in sperm.…”

Section: Gamete Transportmentioning

confidence: 99%

Adhesion molecules in gamete transport, fertilization, early embryonic development, and implantation—role in establishing a pregnancy in cattle: A review

D’Occhio

Campanile

Zicarelli

et al. 2020

Molecular Reproduction Devel

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Cell–cell adhesion molecules have critically important roles in the early events of reproduction including gamete transport, sperm–oocyte interaction, embryonic development, and implantation. Major adhesion molecules involved in reproduction include cadherins, integrins, and disintegrin and metalloprotease domain‐containing (ADAM) proteins. ADAMs on the surface of sperm adhere to integrins on the oocyte in the initial stages of sperm–oocyte interaction and fusion. Cadherins act in early embryos to organize the inner cell mass and trophectoderm. The trophoblast and uterine endometrial epithelium variously express cadherins, integrins, trophinin, and selectin, which achieve apposition and attachment between the elongating conceptus and uterine epithelium before implantation. An overview of the major cell–cell adhesion molecules is presented and this is followed by examples of how adhesion molecules help shape early reproductive events. The argument is made that a deeper understanding of adhesion molecules and reproduction will inform new strategies that improve embryo survival and increase the efficiency of natural mating and assisted breeding in cattle.

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“…In addition to capacitation-associated signaling pathways in the sperm flagellum, super-resolution has been employed to investigate the acrosome reaction. Namely, it has revealed changes in the actin cytoskeleton in the sperm head ( Figure 6E) [228], the distribution of specific integrin heterodimers [234,235], and the formation of SNARE protein complexes immediately prior to acrosome exocytosis [236]. 3D STORM has also revealed unique structures in sperm cells.…”

Section: Novel Technology To Study Trafficking Of Proteins (Super-resmentioning

confidence: 99%

Sperm Differentiation: The Role of Trafficking of Proteins

Teves

Roldán

Krapf

et al. 2020

IJMS

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Sperm differentiation encompasses a complex sequence of morphological changes that takes place in the seminiferous epithelium. In this process, haploid round spermatids undergo substantial structural and functional alterations, resulting in highly polarized sperm. Hallmark changes during the differentiation process include the formation of new organelles, chromatin condensation and nuclear shaping, elimination of residual cytoplasm, and assembly of the sperm flagella. To achieve these transformations, spermatids have unique mechanisms for protein trafficking that operate in a coordinated fashion. Microtubules and filaments of actin are the main tracks used to facilitate the transport mechanisms, assisted by motor and non-motor proteins, for delivery of vesicular and non-vesicular cargos to specific sites. This review integrates recent findings regarding the role of protein trafficking in sperm differentiation. Although a complete characterization of the interactome of proteins involved in these temporal and spatial processes is not yet known, we propose a model based on the current literature as a framework for future investigations.

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Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm

Cited by 14 publications

References 45 publications

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Adhesion molecules in gamete transport, fertilization, early embryonic development, and implantation—role in establishing a pregnancy in cattle: A review

Sperm Differentiation: The Role of Trafficking of Proteins

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