adducin 1 is essential for the survival of erythroid precursors via regulating p53 transcription in zebrafish

Yang, Songbai; Cao, Shan-Hu; Xu, Xuebing; Li, Quan; Li, Jianting; Guo, Jin; Wang, Fang; Bao, Yihua; Jiang, Zongxuan; Zhang, Ting; Wang, Li; Sun, Shao-Guang

doi:10.1016/j.isci.2023.107516

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…In agreement, Epb41-null primitive erythroblasts have membrane defects and reduced deformability [36]. A new study has found an essential role for adducin 1 (add1), encoding another member of the protein 4.1R complex, in zebrafish-primitive (and definitive) erythropoiesis [38]. Homozygous add1 mutants -identified in a gene trap screen -showed only mild reduction of gata1 and embryonic globin expression at 22 hours post fertilization (hpf).…”

Section: Terminal Differentiation Of Primitive Erythroid Cellsmentioning

confidence: 92%

Developmental regulation of primitive erythropoiesis

Rossmann,

Palis

2024

Current Opinion in Hematology

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Purpose of review In this review, we present an overview of recent studies of primitive erythropoiesis, focusing on advances in deciphering its embryonic origin, defining species-specific differences in its developmental regulation, and better understanding the molecular and metabolic pathways involved in terminal differentiation. Recent findings Single-cell transcriptomics combined with state-of-the-art lineage tracing approaches in unperturbed murine embryos have yielded new insights concerning the origin of the first (primitive) erythroid cells that arise from mesoderm-derived progenitors. Moreover, studies examining primitive erythropoiesis in rare early human embryo samples reveal an overall conservation of primitive erythroid ontogeny in mammals, albeit with some interesting differences such as localization of erythropoietin (EPO) production in the early embryo. Mechanistically, the repertoire of transcription factors that critically regulate primitive erythropoiesis has been expanded to include regulators of transcription elongation, as well as epigenetic modifiers such as the histone methyltransferase DOT1L. For the latter, noncanonical roles aside from enzymatic activity are being uncovered. Lastly, detailed surveys of the metabolic and proteomic landscape of primitive erythroid precursors reveal the activation of key metabolic pathways such as pentose phosphate pathway that are paralleled by a striking loss of mRNA translation machinery. Summary The ability to interrogate single cells in vivo continues to yield new insights into the birth of the first essential organ system of the developing embryo. A comparison of the regulation of primitive and definitive erythropoiesis, as well as the interplay of the different layers of regulation – transcriptional, epigenetic, and metabolic – will be critical in achieving the goal of faithfully generating erythroid cells in vitro for therapeutic purposes.

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Section: Terminal Differentiation Of Primitive Erythroid Cellsmentioning

confidence: 92%

Developmental regulation of primitive erythropoiesis

Rossmann,

Palis

2024

Current Opinion in Hematology

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“…17 In recent years, several studies have highlighted the importance of regulating the expression and activity of p53 in primitive erythroid cell differentiation in both mouse and zebrafish embryos. [18][19][20] Thus, it is necessary to reexamine the exact function of BCAS2 in primitive hematopoiesis.…”

Section: Introductionmentioning

confidence: 99%

BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus

Ning,

Lin,

et al. 2024

Preprint

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Breast carcinoma amplified sequence 2 (BCAS2), a core component of the hPrP19 complex, plays an important role in RNA-splicing and DNA damage. However, whether BCAS2 has other functions within the nucleus remains largely unknown. Here, we show that BCAS2 is essential for primitive hematopoiesis in zebrafish and mouse embryos. The activation of Wnt/β-catenin signal, which is required for hematopoietic progenitor differentiation, is significantly decreased upon depletion ofbcas2in zebrafish embryos and mouse embryonic fibroblasts. Interestingly, haploinsufficiency ofbcas2has no obvious impact on the splicing efficiency of β-catenin pre-mRNA, while significantly attenuating β-catenin nuclear accumulation. Moreover, we find that BCAS2 directly binds to β-catenin via its coiled-coil domains, thereby sequestering β-catenin within the nucleus. Thus, our results uncover a previously unknown function of BCAS2 in promoting Wnt signaling by enhancing β-catenin nuclear retention during primitive hematopoiesis.

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Erythropoiesis in Teleost Fishes: The Fantastic Biological Process

Zhong,

Yan

2024

Reviews in Aquaculture

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Global fish production exceeds 170 million tons in 2023, and provides a high‐quality protein source for humans. However, various factors may contribute to hypoxic stress and anemia, which contribute to yield loss in fish production. Elevated red blood cell counts or activation of erythropoiesis are well‐known responses to anemia and hypoxia stress in fish. Erythropoiesis is the process of formation of new erythrocytes, from hemopoietic stem cells, through the differentiation of erythroblasts, to the maturation of red blood cells. This step‐wise process is governed by complex transcriptional, post‐translational, and epigenetic programs in response to extracellular signals. This review summarizes developing knowledge regarding erythropoiesis in teleosts, including the wave and site of erythropoiesis, specification of the erythroid lineage, and molecular regulation mechanisms. A summary of research gaps is presented, to be addressed by future research on teleosts. Understanding erythropoiesis facilitates improved fish husbandry and selective breeding of hypoxia‐tolerance fish, to ultimately contribute to increased production.

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adducin 1 is essential for the survival of erythroid precursors via regulating p53 transcription in zebrafish

Cited by 5 publications

References 46 publications

Developmental regulation of primitive erythropoiesis

Developmental regulation of primitive erythropoiesis

BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus

Erythropoiesis in Teleost Fishes: The Fantastic Biological Process

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