AdeB efflux pump gene knockdown by mRNA mediated peptide nucleic acid in multidrug resistance Acinetobacter baumannii

Abdi, Seyed Naser; Ghotaslou, Reza; Asgharzadeh, Mohammad; Mehramouz, Bahareh; Hasani, Alka; Baghi, Hossein Bannazadeh; Tanomand, Asghar; Narenji, Hanar; Yousefi, Bahman; Gholizadeh, Pourya; Yousefi, Mehdi; Bastami, Milad; Ganbarov, Khudaverdi; Kafil, Hossein Samadi

doi:10.1016/j.micpath.2019.103825

Cited by 20 publications

(12 citation statements)

References 34 publications

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“…baumannii its ability to resist quinolones [ 38 , 39 ]. These quinolone resistance-determining regions are found on codon 80 (serine 80) and 84 (Glu-84) of the parC gene and codon 83 (serine 83) of the gyrA gene [ 40 ]. In this study, all the clinical isolates (100%) were PCR positive for gyrA and 99% positive for parC ( Table 2 ; Figure 3 ; Supplementary 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…Even though literature reports on the plasmid mediated quinolone resistance genes qnrA, qnrB, and qnrS as one of the resistance mechanisms to reduce susceptibility to quinolones [36,37], A. baumannii are mainly resistant to quinolones through chromosomal gene mutation in parC and gyrA [38,39] and other molecular mechanisms [37]. Literature supports that a single mutation in gyrA inducing an amino acid change from serine to leucine in position 83 (serine 83) reduces the susceptibility of A. baumannii clinical isolates to fluoroquinolones [40]. Two mutations in parC in position 80 (serine 80) and 84 (Glu-84) inducing change from serine to isoleucine and glutamic acid into valine, respectively, lead to resistance to fluoroquinolones [40].…”

Section: Introductionmentioning

confidence: 99%

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Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Nogbou

Nkawane

Ntshane

et al. 2021

International Journal of Microbiology

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Acinetobacter baumannii (A. baumannii) has developed several resistance mechanisms. The bacteria have been reported as origin of multiple outbreaks. This study aims to investigate the use of efflux pumps and quinolone resistance-associated genotypic mutations as mechanisms of resistance in A. baumannii isolates at a tertiary hospital. A total number of 103 A. baumannii isolates were investigated after identification and antimicrobial susceptibility testing by VITEK2 followed by PCR amplification of blaOXA-51. Conventional PCR amplification of the AdeABC efflux pump (adeB, adeS, and adeR) and quinolone (parC and gyrA) resistance genes were performed, followed by quantitative real-time PCR of AdeABC efflux pump genes. Phenotypic evaluation of efflux pump expression was performed by determining the difference between the MIC of tigecycline before and after exposure to an efflux pump inhibitor. The Sanger sequencing method was used to sequence the parC and gyrA amplicons. A phylogenetic tree was drawn using MEGA 4.0 to evaluate evolutionary relatedness of the strains. All the collected isolates were blaOXA-51-positive. High resistance to almost all the tested antibiotics was observed. Efflux pump was found in 75% of isolates as a mechanism of resistance. The study detected parC gene mutation in 60% and gyrA gene mutation in 85%, while 37% of isolates had mutations on both genes. A minimal evolutionary distance between the isolates was reported. The use of the AdeABC efflux pump system as an active mechanism of resistance combined with point mutation mainly in gyrA was shown to contribute to broaden the resistance spectrum of A. baumannii isolates.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Nogbou

Nkawane

Ntshane

et al. 2021

International Journal of Microbiology

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“…[32][33][34] The knockdown of AdeB can reduce the resistance to antibiotics in isolates with confirmed other genetic-based mechanisms. 50…”

Section: Rnd Transportersmentioning

confidence: 99%

<p><em>Acinetobacter baumannii</em> Efflux Pumps and Antibiotic Resistance</p>

Abdi

Ghotaslou

Ganbarov

et al. 2020

IDR

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140

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Acinetobacter baumannii is a nosocomial pathogen and gram-negative coccobacillus that is responsible for opportunistic infections, pneumonia, and infections of the urinary tract, bloodstream, skin, and soft tissue. This bacterium poses a major public health problem due to inducing resistance to several drugs, isolates, multidrug treatment, and occasionally pan drugs. Drug resistance is not only a major concern caused by A. baumannii but also is considered as the main challenge in many other pathogens. Several factors such as the efflux pump are associated with antibiotic resistance, biofilm production, and genetic mutations. In this review, A. baumannii is introduced in then some of the practical works conducted on the existing efflux pump are reviewed. The importance of the efflux pump is considered in this paper in relation to the antibiotic resistance and mechanisms developed for the inhibition of these pumps as well.

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“…The RND transporter AcrB associates with the resistance to a variety of antibiotics (Zgurskaya and Nikaido, 1999;Higgins, 2007). The RND transporter A1S_1750 (AdeB), which contains multidrug efflux transporter AcrB transmembrane domain and was layered in k shell of 380, contributes to multidrug resistance of A. baumannii (Abdi et al, 2020a). Thus, it is likely that the genes such as A1S_2736, A1S_2660, and A1S_0116, which have a similar structure of multidrug efflux transporter AcrB transmembrane domain and are simultaneously located in the internal layers of network, are the potential resistance factors (Supplementary Table S5).…”

Section: The Resistance Feature and The Potential Resistance Factorsmentioning

confidence: 99%

Integrated Co-functional Network Analysis on the Resistance and Virulence Features in Acinetobacter baumannii

Xie¹,

Shao²,

Zheng³

2020

Front. Microbiol.

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Acinetobacter baumannii is one of the most troublesome bacterial pathogens that pose major public health threats due to its rapidly increasing drug resistance property. It is not only derived from clinic setting but also emerges from aquaculture as a fish pathogen, which could pass the resistant genes in the food chain. Understanding the mechanism of antibiotic resistance development and pathogenesis will aid our battle with the infections caused by A. baumannii. In this study, we constructed a co-functional network by integrating multiple sources of data from A. baumannii and then used the k-shell decomposition to analyze the co-functional network. We found that genes involving in basic cellular physiological function, including genes for antibiotic resistance, tended to have high k-shell values and locate in the internal layer of our network. In contrast, the non-essential genes, such as genes associated with virulence, tended to have lower k-shell values and locate in the external layer. This finding allows us to fish out the potential antibiotic resistance factors and virulence factors. In addition, we constructed an online platform ABviresDB (https://acba.shinyapps.io/ABviresDB/) for visualization of the network and features of each gene in A. baumannii. The network analysis in this study will not only aid the study on A. baumannii but also could be referenced for the research of antibiotic resistance and pathogenesis in other bacteria.

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AdeB efflux pump gene knockdown by mRNA mediated peptide nucleic acid in multidrug resistance Acinetobacter baumannii

Cited by 20 publications

References 34 publications

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

<p><em>Acinetobacter baumannii</em> Efflux Pumps and Antibiotic Resistance</p>

Integrated Co-functional Network Analysis on the Resistance and Virulence Features in Acinetobacter baumannii

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