Adenosine receptor protein changes in guinea pigs with form deprivation myopia

Abstract

Acta Ophthalmol. 2010: 88: 759–765 Abstract. Purpose: Recent results have shown that treatment with the non‐selective adenosine antagonist 7‐methylxanthine (7‐MX) reduces the development of form deprivation myopia (FDM) in guinea pigs. The aims of this study were to identify the presence of adenosine receptors (AdoRs) in the eye wall of the guinea pig and to determine their possible changes during form deprivation. Methods: Three‐week‐old guinea pigs were monocularly treated with a translucent lens to induce FDM. After 21 days, samples were taken from the posterior eye wall and examined with immunofluorescence confocal microscopy for the presence of AdoRA1, AdoRA2A, AdoRA2B and AdoRA3 proteins. Western blot analysis was used to quantitate AdoRs in samples from the retina, choroids and sclera. Results: All four subtypes of AdoR were expressed in the posterior wall of the guinea pig eye, although AdoRA3 only weakly. Twenty‐one days after the induction of myopia, we observed a significant decrease in protein expression for AdoRA1 (− 25.5%) and an increase in protein expression for AdoRA2B (+ 66.7%) in the retina of FDM eyes. Conclusions: AdoRs of all subtypes are expressed in the retina, choroids and sclera in guinea pigs and may play a role in the regulation of eye growth. The changed pattern of AdoR expression during form deprivation confirms that pharmaceutical intervention targeting AdoRs may reduce myopia progression.

Gene expression signatures in tree shrew choroid during lens-induced myopia and recovery

“…The purinergic receptor gene P2RY1 was up-regulated in GO and STAY; however, ADORA2A (adenosine receptor A2a) was not significantly affected, a similar result to that found at the protein level in form-deprived guinea pig choroid (Cui et al, 2010). …”

“…While a number of changes were found, it is not possible to know if the changes occurred in the RPE or choroid. In tree shrews (mammals closely related to primates; Luckett, 1980) and guinea pigs, studies have examined changes of a few genes and proteins in the choroid in form-deprived animals (Cui et al, 2010; Jobling et al, 2009; Liu et al, 2007; McBrien et al, 2009). In the present study, we examined, in the choroid, changes in gene expression of a large sample of genes in animals treated with a minus lens.…”

Adenosine receptors and caffeine in retinopathy of prematurity

“…A 1 , A 2A , A 2B and A 3 ) (Fredholm et al, 2001) to exert control over blood vessel growth in various tissues, including retina, both under normal and pathological conditions (Adair et al, 2005; Patz, 1980). All four adenosine receptor subtypes have been detected in retina (Cui et al, 2010; Brito et al, 2012). …”

Adenosine A2AR modulates cardiovascular function by activating ERK1/2 signal in the rostral ventrolateral medulla of acute myocardial ischemic rats

“…The concentration of total proteins extracted from those tissue samples was determined by the bicinchoninic acid (BCA) assay. Protein samples (30 μg) were separated by 12% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride membranes (Gelman-Pall, Ann Arbor, MI, USA), blocked with 5% nonfat milk, and incubated with either rabbit polyclonal antibodies against A 2A R (1:1000) (Cui et al, 2010), or rabbit polyclonal antibodies against pERK1/2 (1:1000), or rabbit polyclonal antibodies against ERK1/2 (1:1000), or mouse polyclonal antibodies against β-actin (1:3000) at in 5% nonfat milk, and incubated at 4°C overnight. Mouse polyclonal antibodies against β-actin were incubated as an internal standard.…”