Adenovirus 37: Identification and characterization of a medically important new adenovirus type of subgroup D

Abstract

A new human adenovirus has been isolated from 62 eyes with (kerato)conjunctivitis and from nine genitourinary sites. The virus is closely related in haemagglutination inhibition tests to adenovirus type 19 (Ad 19) and Ad 10. Antiserum adsorption experiments demonstrated the presence of three haemagglutinin antigens in the virus: One unique, another common to Ad 19, and a third common to Ad 10 and 19. In neutralization tests, the virus is distantly related to Ad 13, 30, 19, and 10. Despite this relationship, it is proposed to call the virus adenovirus 37, in agreement with current species definitions. It belongs to subgroup D of human adenoviruses. Antisera to the new virus show virtually no neutralization of other human adenovirus types. Only bay use of this antiserum it is in practice possible to avoid wrong or indefinite typing, which has often occurred in the past.

A Nosocomial Outbreak of Epidemic Keratoconjunctivitis due to Adenovirus Type 37

“…Some cross-reaction with Ad-19 was observed. NT cross-reaction between Ad-37 and Ad-19 has been reported by previous workers (1,6 ).…”

The Arg279Glu Substitution in the Adenovirus Type 11p (Ad11p) Fiber Knob Abolishes EDTA-Resistant Binding to A549 and CHO-CD46 Cells, Converting the Phenotype to That of Ad7p

“…The fiber consists of a tail, shaft, and knob domain (8). Trimerization of the fiber protein is noncovalent and dependent on ␤-sheet structures in the shaft, while the globular knob domain consists of an eight-to nine-stranded antiparallel ␤-sandwich connected by loops (9,34). Each of the eight or nine strands is named A to H or A to J, respectively, while the loops are referred to as AB, CD, EF, etc.…”

“…However, species D serotypes 8,19, and 37 (causing epidemic keratoconjunctivitis) have been shown to bind sialic acid instead of CAR (1,3,9,11,17). Recently it has been suggested that Ad37 can also bind to CD46 (44).…”

Human Adenovirus from Subgenus D: Restriction Mapping of Types 9 and 19 and Characterization of a New Genome Type

“…They are non-oncogenic and are capable of complete agglutination of rat cells. These viruses, especially type 19, have been described as causal agents of ophthalmic diseases, such as keratoconjunctivitis (Dawson et al, 1970;Desmyter et al, 1974;Hierholzer et al, 1974;Guyer et al, 1975;Darougar et al, 1977;Wadell & de Jong, 1980;de Jong et al, 1981 ;Keenlyside et al, 1983;Kemp et al, 1983). Their genomes contain about 58% GC and the homology between the DNAs of the subgenus D AVs is o/ 94 to 99/o.…”