Adenovirus DNA Replication

Liu, H.; Naismith, J.H.; Hay, Ronald T.

doi:10.1007/978-3-662-05597-7_5

Cited by 44 publications

(47 citation statements)

References 156 publications

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“…Proteins encoded by the adenovirus E2 genes are essential for viral DNA replication (de Jong et al, 2003;Liu et al, 2003). Adenoviral DNA replication is initiated by a protein priming mechanism involving the preterminal protein (pTP), which is covalently attached to the 5 0 end of the genomic DNA.…”

Section: Adenovirus Gene Expression and Replicationmentioning

confidence: 99%

Inactivating intracellular antiviral responses during adenovirus infection

2005

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DNA viruses promote cell cycle progression, stimulate unscheduled DNA synthesis, and present the cell with an extraordinary amount of exogenous DNA. These insults elicit vigorous responses mediated by cellular factors that govern cellular homeostasis. To ensure productive infection, adenovirus has developed means to inactivate these intracellular antiviral responses. Among the challenges to the host cell is the viral DNA genome, which is viewed as DNA damage and elicits a cellular response to inhibit replication. Adenovirus therefore encodes proteins that dismantle the cellular DNA damage machinery. Studying virus-host interactions has yielded insights into the molecular functioning of fundamental cellular mechanisms. In addition, it has suggested ways that viral cytotoxicity can be exploited to offer a selective means of restricted growth in tumor cells as a therapy against cancer. In this review, we discuss aspects of the intracellular response that are unique to adenovirus infection and how adenoviral proteins produced from the early region E4 act to neutralize antiviral defenses, with a particular focus on DNA damage signaling.

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Section: Adenovirus Gene Expression and Replicationmentioning

confidence: 99%

Inactivating intracellular antiviral responses during adenovirus infection

2005

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“…Adenoviruses contain a linear dsDNA genome of approximately 36 kb that is replicated in the nucleus of the host cell (Liu et al, 2003;Shenk, 2001). The adenovirus DNA polymerase catalyses the covalent linkage of 5Ј terminal nucleotide dCMP to a serine residue on the viral preterminal protein (preTP).…”

Section: Introductionmentioning

confidence: 99%

“…This complex binds to the termini of the linear viral DNA and initiates replication of the DNA using the preTP-dCMP molecule as a primer. This occurs in the presence of cellular factors such as NF-1 and Oct-1, which enhance the rate of initiation by binding specific sequences and encouraging unwinding of the viral origin of replication (de Jong et al, 2003;Liu et al, 2003). Once initiated, elongation occurs adjacent to centres in the nucleus that are rich in the viral ssDNA binding protein called DBP.…”

Section: Introductionmentioning

confidence: 99%

Nucleolar protein upstream binding factor is sequestered into adenovirus DNA replication centres during infection without affecting RNA polymerase I location or ablating rRNA synthesis

Lawrence

McStay

Matthews

2006

Journal of Cell Science

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When human adenovirus infects human cells there is disruption of rRNA biogenesis. This report examines the effect of adenovirus infection on the nucleolar protein, upstream binding factor (UBF) which plays a major role in regulating rRNA synthesis. We determined that early after infection, UBF associates with the replication of viral DNA, preferentially associating with the ends of the linear viral genome, and that addition of anti-UBF serum to in vitro replication assays markedly reduced viral DNA replication. Regions of UBF important to these observations are also established. Interestingly, sequestering the majority of UBF from the nucleolus did not lead to the ablation of rRNA synthesis or the sequestration of RNA pol I. In infected cells the bulk of RNA synthesis was RNA pol I associated and distinct from the location of most of the detectable UBF. We propose that UBF plays a role in viral DNA replication, further strengthening the role of nucleolar antigens in the adenovirus life cycle.

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“…This implies that HAdV_Chiba_ E086/2012 originated from a HAdV-GT59-like virus, because it is less likely that these segments were independently copied from HAdV-GT59 simultaneously so as to satisfy the complementarity constraints on these terminal regions (28,29). The results of our analysis also indicate that HAdV_Chiba_E086/2012 has experienced several recombination events with HAdV-48, HAdV-GT65, HAdV-22, HAdV-29, and HAdV-GT60 or their recombinant viruses to form the current HAdV_Chiba_ E086/2012 genome as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

A Novel Complex Recombinant Form of Type 48-Related Human Adenovirus Species D Isolated in Japan

et al. 2014

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SUMMARY:Recently, new genotypes of human adenoviruses (HAdVs) have been reported and many of them have been found to be recombinant forms of different known types of HAdV species D (HAdV-D). The objective of this study was to document the evolutionary features of a novel isolate (HAdV_ Chiba_E086/2012) obtained from the eye swab of a patient with conjunctivitis in Japan. Viral DNA was extracted from the isolate to sequence the whole genome by the Sanger method and aligned with available genome sequences of HAdV-Ds. The phylogenetic trees of the nucleotide sequences of the penton base, hexon, and fiber genes and the E3 region showed that HAdV_Chiba_E086/2012 is closest to HAdV genotype 65 (HAdV-GT65), HAdV-48, HAdV-GT60 and HAdV-22 at 98z, 99z, 95z and 98z identity, respectively, suggesting that this isolate is a novel recombinant form to be designated as P65H48F60. Further phylogenetic and recombination analyses of the genome alignment of the new isolate implied that nested recombination events involving HAdV-GT59, GT65, 48, GT60, 22, and some ancestral lineages or their close relatives have shaped its genome. These results showed that HAdV_ Chiba_E086/2012 is the first HAdV-48-related HAdV found in Japan, which has the most complicated evolutionary history among the known HAdVs so far.

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Adenovirus DNA Replication

Cited by 44 publications

References 156 publications

Inactivating intracellular antiviral responses during adenovirus infection

Inactivating intracellular antiviral responses during adenovirus infection

Nucleolar protein upstream binding factor is sequestered into adenovirus DNA replication centres during infection without affecting RNA polymerase I location or ablating rRNA synthesis

A Novel Complex Recombinant Form of Type 48-Related Human Adenovirus Species D Isolated in Japan

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