Adenylate Cyclase Activity During Phenotypic Variation of Bordetella pertussis

Brownlie, Robert; Coote, J. G.; Parton, R.

doi:10.1099/00221287-131-1-27

Cited by 6 publications

(7 citation statements)

References 11 publications

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“…Well studied virulence factors include filamentous haemagglutinin (FHA), which is involved in bacterial adhesion, and pertussis toxin (PT) and adenylate cyclase, which are involved in evasion of the host defenses and intoxication of the eukaryotic host (Weiss et al 1983(Weiss et al , 1984. A number of environmental stresses, such as temperature and chemical agents, are known to control the transcription of the virulence genes in B.pertussis (Weiss et al, 1983(Weiss et al, , 1984Brownlie et al, 1985;Gross and Rappuoli, 1988;Arico et al, 1989;Gross et al, 1989;Melton and Weiss, 1989;Miller et al 1989a,b;Roy et al, 1989). The expression of the virulence factors in B.pertussis is co-ordinately regulated by the bvg locus which codes for the regulatory proteins BvgA and BvgS (originally called BvgABC).…”

mentioning

confidence: 99%

Sequential activation and environmental regulation of virulence genes in Bordetella pertussis.

Scarlato¹,

Aricò²,

Prugnola³

et al. 1991

The EMBO Journal

127

146

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Bacterial pathogens undergo profound physiological changes when they infect their host and require coordinated regulation of gene expression in response to the stress encountered during infection. In Bordetella pertussis, the human pathogen which causes whooping cough, virulence factors are synthesized in response to environmental signals under the control of the bvg regulatory locus. Here we demonstrate that the bvg locus is responsible for two events of gene activation. In the first step the bvg locus transactivates its own autoregulated promoter (P1) and the promoter of the adherence factor filamentous haemagglutinin (PFHA).The second step occurs several hours later and consists of the transactivation of adenylate cyclase and pertussis toxin genes. We provide evidence that the second step of transactivation requires overexpression of regulatory proteins. Our results imply that bacterial adhesion and tissue colonization -intoxication are two separate steps at the molecular level.

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mentioning

confidence: 99%

Sequential activation and environmental regulation of virulence genes in Bordetella pertussis.

Scarlato¹,

Aricò²,

Prugnola³

et al. 1991

The EMBO Journal

127

146

View full text Add to dashboard Cite

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“…This may have had the effect of causing the B. pertussis cells to undergo a phenotypic change known as antigenic modulation (Lacey, 1960), involving changes in envelope proteins and other surface properties (Parton & Wardlaw, 1975;Wardlaw & Parton, 1979). The threshold concentration at which antigenic modulation occurs is 10 to 11 rn~-MgSo, (Brownlie et al, 1985). In the present investigation, the presence of 20 mM-MgSO, was found to have essentially no effect on the plasmid transfer frequency of RPl , RP1: : Tn501, R68.45 or RP4 : : Tn7 from E. coli JC3272 to B. pertussis BPT2 (results not shown).…”

Section: Resultsmentioning

confidence: 49%

R-Plasmid-mediated Chromosome Mobilization in Bordetella pertussis

Smith¹,

Coote²,

Parton³

1986

Microbiology

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~~Antibiotic-resistant and auxotrophic mutants of Bordetella pertussis were isolated. These were used as recipients for the uptake from Escherichia coli of broad-host-range R plasmids R68.45, RPl, and RP1 and RP4 carrying transposons Tn.501 and Tn7 respectively. B. pertussis transconjugants from these crosses were used as donors to mobilize StrR, NalR, thr+ and sly+ chromosomal markers to B. pertussis or to B. parapertussis recipient strains. The frequency of plasmid transfer varied and depended on the donor and recipient strains used. Differences in chromosome mobilization frequencies of individual markers were observed and appeared to depend on the presence or absence of transposons Tn501 and Tn7 on the plasmid. Linkage was detected between the gly+ and NalR markers.

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“…Lacey (1960) reported that this concentration of MgC12, and other Mg salts present in Bordet-Gengou medium, caused modulation in terms of antigenic changes, although he reported MgCI2 to be a weak pro-C-mode salt compared to MgSO,. In our accompanying study, the critical concentration of MgSO, required to induce loss of the X polypeptides in strain TAB I was 10 to 1 1 mM (Brownlie et al, 1984). These observations suggest that the important component of MgSO, for inducing modulation is the SO:anion.…”

Section: Ss-x Medium Andmentioning

confidence: 69%

“…Results of this study, where loss of HSA and the X polypeptides occurred concomitantly with the loss of adenylate cyclase activity during modulation induced by different inorganic and organic salts, does not detract from this suggestion. However, further studies (Brownlie et al, 1984) indicate that this enzyme does not play a causal role in the loss of virulence-related factors during modulation, but rather that both are influenced by the same control mechanism which underlies the modulation phenomenon.…”

Section: Ss-x Medium Andmentioning

confidence: 93%

The Effect of Growth Conditions on Adenylate Cyclase Activity and Virulence-related Properties of Bordetella pertussis

Brownlie¹,

Parton²,

Coote³

1985

Microbiology

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Growth of Bordetella pertussis in Stainer & Scholte medium in which the NaCl had been replaced by one of several inorganic or organic salts resulted in a large decrease in adenylate cyclase activity, histamine-sensitizing activity and in the amounts of two cell-envelope polypeptides of Mr 28000 and 30000. Although some variation between strains was observed, there was never a case where one of these properties was lost independently of the others. Cultures in which these properties were lost had decreased amounts of extracellular cAMP when compared to NaCl-grown cultures. Adenylate cyclase activity was detected in three locations of B. pertussis cultures (extracellular, extracytoplasmic but cell-associated, and cytoplasmic). After growth in medium containing high concentrations of MgSO4, enzyme activity was decreased to a similar extent in all three locations.

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Adenylate Cyclase Activity During Phenotypic Variation of Bordetella pertussis

Cited by 6 publications

References 11 publications

Sequential activation and environmental regulation of virulence genes in Bordetella pertussis.

Sequential activation and environmental regulation of virulence genes in Bordetella pertussis.

R-Plasmid-mediated Chromosome Mobilization in Bordetella pertussis

The Effect of Growth Conditions on Adenylate Cyclase Activity and Virulence-related Properties of Bordetella pertussis

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