Adherence to a metal, polymer and composite by Staphylococcus aureus and Staphylococcus epidermidis

Verheyen, Cees C. P. M.; Dhert, W.J.A.; Blieck-Hogervorst, J.M.A. de; Reijden, Tanny J. K. van der; Petit, P. L. C.; Groot, K. de

doi:10.1016/0142-9612(93)90059-b

Cited by 54 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparison of these values suggests that S.epidermidis had the greatest rate of adhesion and this regardless of the polymer. It is consistent with the results of previously reported studies [16,17]. On the other hand, E.coli exhibited the smallest rate of adhesion to polymers.…”

Section: Resultssupporting

confidence: 94%

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Karakeçili¹,

Gümüşderelı́oğlu²

2002

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

In this study, interactions of widely-used polymeric biomaterials, i.e. poly(hydroxyethyl methacrylate) (PHEMA) and its copolymer with dimethylaminoethyl methacrylate (PHEMA-20% DMAEMA), polyurethane (PU), polypropylene (PP), poly(vinyl chloride) (PVC), and poly(lactide-glycolide) (PLGA), with three pathogenic bacteria and one nonpathogen were investigated comparatively with the adhesion of two tissue cells in different morphologies, i.e. fibroblast-like baby hamster kidney (BHK 21) cells and epithelial Madine Darby kidney (MDBK) cells. Biomaterials were prepared in the membrane form by bulk polymerization or solvent casting. Surface characterization studies showed that these polymers have different surface free energies in the range of 26.9-63.1 erg cm(-2) and they have smooth surfaces. The bacteria used were; Escherichia coli ATCC 25922, Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus, and Lactobacillus acidophilus B-13. Initial adhesion of bacteria to the polymeric surfaces was examined under static conditions and in a laminar flow cell. The adhesion behaviour of S. aureus and S. epidermidis was found independent of the polymeric surface hydrophobicity. However, the percentage of attached E. coli decreased when increasing the surface free energy of the polymer, while L. acidophilus showed just the opposite behaviour. The comparative results indicated that the adhesion of BHK and MDBK cell was lowest on the most hydrophilic PHEMA surface and highest on the most hydrophobic PP surface. In contrast to the case of bacterial adhesion, no relationship was found between polymer hydrophobicity and mammalian cell adherence.

show abstract

Section: Resultssupporting

confidence: 94%

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Karakeçili¹,

Gümüşderelı́oğlu²

2002

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

show abstract

“…They reported that the roughest material promoted biofilm accumulation significantly more than the other, smoother materials. Other researchers have found similar effects of increased bacterial colonization on rougher biomaterials (3,37). In contrast, there are reports indicating no difference in bacterial adhesion with roughened surfaces (21,39).…”

Section: Discussionmentioning

confidence: 51%

“…However, no differences could be detected when S. mitis adherence was tested on the surfaces of the samples used in this study. S. mitis is one of the major bacteria in the oral indigenous microflora and is not capable of producing glucans, as no gft genes have been reported to be present in any strains of S. mitis (24,37). Evidently biofilm adherence was enhanced by WIG synthesized by S. mutans, but WIG-free S. mitis colony adhesion onto the material surfaces was also observed.…”

Section: Discussionmentioning

confidence: 89%

Surface Response of Fluorine Polymer-Incorporated Resin Composites to Cariogenic Biofilm Adherence

Gyo

Nikaido

Okada

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

Experimental resin composites with incorporated polytetrafluoroethylene (PTFE) particles were developed, which theoretically could improve the surface properties of the materials, including the inhibition of bacterial adherence. To assess the surface properties in relation to biofilm formation and detachment, 23.1% (wt/wt) linear PTFE particles (FL-30) and cross-linked PTFE particles (FC-30) were incorporated into pure resin composites. Pure PTFE plates and pure resin composites without PTFE (F-0) were used as control specimens. Sucrose-dependent Streptococcus mutans biofilms were formed on the specimen blocks inside an oral biofilm reactor for various time periods and analyzed with or without application of driving forces. In addition, water contact angles and surface roughness were measured. The water contact angles of FL-30 (61.2°) and FC-30 (65.8°) were larger than that of F-0 (48.5°). The largest contact angle (107°) was detected on pure PTFE plates. However, the surfaces of FL-30, FC-30, and pure PTFE plates were rougher than that of F-0. Although the surface properties of the materials differed in terms of contact angles and roughness, these factors seemed not to affect biofilm formation on the surfaces within 5 h. Pure PTFE plates harbored almost the same amounts of biofilm as F-0. However, when a very strong driving force was applied, it was clear that there were significantly smaller amounts of biofilms retained on pure PTFE plates, which showed contact angles much higher than those of the other materials. Hydrophobicity of the resin composite was improved by incorporation of PTFE fillers. However, surface resistance against biofilm formation was not improved.

show abstract

“…Direct microscopic observation methods of microbial cells adherent to substrata based on the use of optical, scanning electron, and transmission electron microscopes also have been employed, [11][12][13] but these methods present difficulties in assessing cell viability and in counting aggregated bacteria.…”

Section: Introductionmentioning

confidence: 99%

Quantitative evaluation of bacteria adherent to polyelectrolyte HEMA‐based hydrogels

Berlutti

Rosso

Bosso

et al. 2003

J Biomedical Materials Res

View full text Add to dashboard Cite

The use of adhesive poly(HEMA)-based hydrogels is standard practice in dental restorative procedures. Microorganisms, which potentially can cause oral pathologies, may colonize these polymers. In the present work, bacterial adhesion to polymers prepared with 2-hydroxyethyl methacrylate (HEMA) and to different molar ratios of 2-acrylamido-2-methylpropane-sulfonic acid (AMPS) and/or to 2-methacryloyloxyethyl-tri-methyl-ammonium chloride (METAC) co-monomers were tested. A colorimetric assay system that utilizes the Microbo revelation medium (Microbo srl, Rome, Italy) for microbial counts is shown to be capable of counting the number of adherent bacterial cells without removing them from polymer surfaces. In conditions that mimic those present in the oral cavity, similar bacterial adhesion percentages on the same polymer were observed with the different bacteria belonging to both gram-positive and gram-negative genera, such as Streptococcus sobrinus and Streptococcus oralis (resident microorganisms in the oral cavity) and Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa (transient microorganisms in the oral cavity). It is determined that the physico-chemical characteristics of poly(HEMA)-based hydrogels are the major factors promoting bacterial adhesion, which increased with increasing water content in the swollen polymers, reaching maximal values on the cationic polymers.

show abstract

Adherence to a metal, polymer and composite by Staphylococcus aureus and Staphylococcus epidermidis

Cited by 54 publications

References 28 publications

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Surface Response of Fluorine Polymer-Incorporated Resin Composites to Cariogenic Biofilm Adherence

Quantitative evaluation of bacteria adherent to polyelectrolyte HEMA‐based hydrogels

Contact Info

Product

Resources

About