Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria

Zawistowska-Rojek, Anna; Kośmider, Anita; Stępień, Karolina; Tyski, Stefan

doi:10.1007/s00203-022-02889-8

Cited by 48 publications

(35 citation statements)

References 47 publications

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“…Characterizing important changes in this family in KPbA mice may ultimately provide promising microbial targets to combat the metabolic disorders caused by malaria. As one of the most common probiotic families (Zawistowska-Rojek et al, 2022), Lactobacillaceae was significantly lower in the WPbA and KPbA groups than in their corresponding control groups, but was less affected by MIF knockout, indicating that it is mainly PbA infection that caused the decrease in Lactobacillaceae.…”

Section: Discussionmentioning

confidence: 89%

Deficiency of migration inhibitory factor influences the gut microbiota of C57BL/6 mice infected with Plasmodium berghei ANKA

et al. 2022

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Cerebral malaria (CM), as one of the most common complications in severe malaria, has threatened millions of individuals’ neurological health and even their lives. Macrophage migration inhibitory factor (MIF), a pleiotropic proinflammatory factor in humans, seems to be a risk factor for death in patients with CM, but its functional mechanism remains unclear. To verify whether affecting the intestinal microbes of the host was one of the mechanisms by which MIF regulates CM, C57BL/6 mice, including WT + PbA, MIF-KO + PbA and their uninfected controls, were sent for 16S rRNA-based sequencing targeting the V4 region of the intestinal microbiota through the Illumina MiSeq platform. The results showed that OTU clustering, alpha and beta diversity in the four groups involved had evident variation. The relative abundance at different taxonomic levels, especially the dominant intestinal flora, was obviously changed. The LEfSe analysis screened out several biomarkers, including significantly reduced Ligilactobacillus (Lactobacillus murinus) in WPbA mice compared to the WT group and Akkermansia (Akkermansia_muciniphila) in KPbA mice compared to the WPbA group. For MIF KO groups, mice infected with PbA or uninfected showed significant enrichment of producers of short-chain fatty acids, including Dubosiella and Faecalibaculum (Faecalibaculum rodentium) in KPbA, and Lachnospiraceae_NK4A136_group and Firmicutes_bacterium_M10-2 in KO. This study not only further proved the gut microbiota changes in C57BL/6 mice caused by PbA infection, but also found that MIF deletion directly affected the changes in the gut microbiota of C57BL/6 mice before and after PbA infection. This finding reveals a potential mechanism by which MIF regulates CM. Combining MIF with potential microbial biomarkers will provide a promising idea to develop combined drugs for improving CM in the future.

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Section: Discussionmentioning

confidence: 89%

Deficiency of migration inhibitory factor influences the gut microbiota of C57BL/6 mice infected with Plasmodium berghei ANKA

et al. 2022

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“…Of the notable taxa that differed between paired and isolated voles, Anaerostipes and Lactobacillaceae , which had a greater abundance in the fecal communities of paired voles, have previously been associated with putative benefits to host health either through the production of short chain fatty acids (SCFAs) [ 43 , 44 , 45 ] or regulation of pathogen growth and colonization [ 23 , 46 ]. Conversely, several taxa, such as Enterococcus , Metaprevotella , and Staphylococcaceae that had a greater abundance in the fecal communities of isolated voles have previously been associated with disease [ 35 , 42 , 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiome and Metabolome Variation as Indicator of Social Stress in Female Prairie Voles

Nuccio

Normann

Zhou

et al. 2023

IJMS

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Social isolation is detrimental to the health of social mammals inducing neurochemical and hormonal changes related to depression and anxiety, as well as impairments of cardiovascular and immune functioning. Likewise, perceptions of loneliness are increasingly recognized as detrimental to human psychological well-being, cognitive functioning, and physical health. Few studies, however, have examined the impact of social isolation on the intestinal microbiome and metabolome. To better understand the impact of social isolation on these systems, intestinal microbiota, and the systemic impact via the gut–brain axis, we employed prairie voles. Physiological stress on female prairie voles (n = 22) either with a same-sex sibling (n = 11) or in isolation (n =11) for four weeks demonstrated behavioral indicators of increased anxiety and depression in isolated voles (p ≤ 0.01). Bacterial DNA from fecal and colon samples, collected at five time points (T0–4), were sequenced for all nine hypervariable regions of the 16S rRNA gene. Microbiome analyses revealed several differences in gut communities of paired and isolated voles with greater differences at T4. Notably, several taxa associated with host health including Anaerostipes and Lactobacillaceae were more prevalent in paired voles, whereas several taxa associated with known pathogens (e.g., Staphylococcaceae and Enterococcus) or disease were elevated in isolated animals. Similarly, metabolome analyses suggested isolated voles, when compared to paired animals, exhibited differences in metabolites associated with diabetes and colitis. These findings further contribute to our understanding of the harmful effects of social isolation, which cause perturbations in the gut microbiome and serum metabolites.

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“…The ability to auto-aggregate lactobacilli is an important feature that creates the barrier and hinders patho-genic strains' adhesion (Klopper et al 2018). The value of auto-aggregation depends mainly on the incubation time (Piwat et al 2015;) and on the strain tested (Zawistowska-Rojek et al 2022b). In the current study, the auto-aggregation values ranged from 37% to 89%, depending on the strain used.…”

Section: Lacticaseibacillusmentioning

confidence: 99%

“…In addition, it is suggested that the shape of the bacterial cell is also vital for aggregation; longer cells, due to their larger surface area, are characterised by more significant aggregation. Thanks to this, lactic acid bacterial cells can remain in the host's gastrointestinal tract for a long time (Rajab et al 2020;Zawistowska-Rojek et al 2022b). According to some researchers, bacterial auto-aggregation is correlates with these bacteria's ability to adhere to and colonise the gastrointestinal tract (Hojjati et al 2020).…”

Section: Introductionmentioning

confidence: 99%

New Potentially Probiotic Strains Isolated from Humans – Comparison of Properties with Strains from Probiotic Products and ATCC Collection

Zawistowska-Rojek

Kociszewska

Zaręba

et al. 2022

Polish Journal of Microbiology

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Lactic acid bacteria are used in various types of probiotic products. Due to the constantly growing probiotics market, new strains with pro-health properties are sought. The present study compared 39 strains of Lactobacillus, Lacticaseibacillus, and Lactiplantibacillus, isolated from probiotic products and healthy people. The current research aimed to search for new, potentially probiotic strains. For this purpose the relationship between Lactobacillaceae strains was carried out; moreover, the basic properties of probiotic microorganisms, such as survival at low pH and bile salt environment, antibiotic susceptibility, aggregation and antagonism were estimated. The properties of these isolates were also compared with the properties of probiotic strains from the ATCC collection. In comparing the genetic relationship (PFGE method) between the tested isolates, it was observed that some of them show a high degree of similarity. All tested strains tolerated an environment with a pH value of 3.0, and the addition of 0.3% bile salt; showed auto-aggregation properties and displayed antagonism against pathogenic microorganisms. In the present study, the bacteria were susceptible to tetracycline, chloramphenicol and ampicillin; the resistance to vancomycin depended on the bacteria type. All the properties were strain-depended. Most of the tested strains had properties comparable to the reference strains. Three L. acidophilus strains isolated from cervical swabs seem to be promising candidates for probiotic strains.

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Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria

Cited by 48 publications

References 47 publications

Deficiency of migration inhibitory factor influences the gut microbiota of C57BL/6 mice infected with Plasmodium berghei ANKA

Deficiency of migration inhibitory factor influences the gut microbiota of C57BL/6 mice infected with Plasmodium berghei ANKA

Microbiome and Metabolome Variation as Indicator of Social Stress in Female Prairie Voles

New Potentially Probiotic Strains Isolated from Humans – Comparison of Properties with Strains from Probiotic Products and ATCC Collection

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