Adhesion and Internalization of Functionalized Polystyrene Latex Nanoparticles toward the Yeast Saccharomyces cerevisiae

Miyazaki, Jumpei; Kuriyama, Yuta; Miyamoto, Akihisa; Tokumoto, Hayato; Konishi, Yasuhiro; Nomura, Toshiyuki

doi:10.4164/sptj.50.472

Cited by 5 publications

(13 citation statements)

References 29 publications

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“… : Microplastics as trigger of combined physical or chemical‐like effects. Soil biogeochemistry related to agricultural mulching (Steinmetz et al., ), ingestion by terrestrial and continental birds (Gil‐Delgado et al., ; Holland et al., ; Zhao et al., ), reduction in growth of earthworms (Lwanga et al., ), lethal toxicity to fungi (Miyazaki et al., , ; Nomura et al., ), mammal lung inflammation (Hamoir et al., ; Oberdorster, ; Schmid & Stoeger, ) and broad cytotoxicity (Forte et al., ; Kato et al., ) of nanoplastics [Colour figure can be viewed at wileyonlinelibrary.com]…”

Section: Mechanisms Of Potential Impacts Of Microplastic On Terrestrimentioning

confidence: 99%

“…Moreover, microplastics could act as vector of toxic Zn to earthworms under environmental conditions due to a higher adsorption of this metal to high density polyethylene microplastic (Hodson et al., ). Lethal effects (100% mortality) were observed after 1 h exposure of yeast cells of Saccharomyces cerevisiae to polystyrene nanobeads (50 and 100 nm, 10–15 mg/L) in 5 mM NaCl culture media (Miyazaki et al., ). For the filamentous fungi Aspergillus oryzae and Aspergillus nidulans the nanoplastic toxicity was not uniform among species or phenotypes, which was explained by the variability in one single trait: the resistance and hydrophobicity of cell walls (Nomura et al., ).…”

Section: Mechanisms Of Potential Impacts Of Microplastic On Terrestrimentioning

confidence: 99%

“…Nanoplastics externally adsorbed to cells might still cause toxicity by disrupting several membrane processes essential to the intracellular homeostasis of the exposed organism. Amino terminated polystyrene beads (50 nm) could strongly adhere to cells causing high toxicity at concentrations around 10 mg/L (Miyazaki et al., ). Conversely, carboxyl terminated polystyrene particles (50 nm) did not enter eukaryotic cells ( S. cerevisiae ), which had little effects on the growth rate at up to 80 mg/L after 1 h exposure (Miyazaki et al., ).…”

Section: The Broad‐spectrum Toxicity Targets Of Nanoplasticsmentioning

confidence: 99%

“…Carboxyl ( COOH ) and amino ( NH 2 ) terminated or lecithin coated polystyrene beads yield nanoparticles with diverse cellular fate, which influences the toxicity mechanism. Reported processes (Kato et al., ; Miyazaki et al., , ; Syberg et al., ) do not fully explain toxicity and uptake. Further mechanisms await discovery [Colour figure can be viewed at wileyonlinelibrary.com]…”

Section: The Broad‐spectrum Toxicity Targets Of Nanoplasticsmentioning

confidence: 99%

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Microplastics as an emerging threat to terrestrial ecosystems

Machado

Kloas

Zarfl

et al. 2018

Global Change Biology

1,619

537

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Microplastics (plastics <5 mm, including nanoplastics which are <0.1 μm) originate from the fragmentation of large plastic litter or from direct environmental emission. Their potential impacts in terrestrial ecosystems remain largely unexplored despite numerous reported effects on marine organisms. Most plastics arriving in the oceans were produced, used, and often disposed on land. Hence, it is within terrestrial systems that microplastics might first interact with biota eliciting ecologically relevant impacts. This article introduces the pervasive microplastic contamination as a potential agent of global change in terrestrial systems, highlights the physical and chemical nature of the respective observed effects, and discusses the broad toxicity of nanoplastics derived from plastic breakdown. Making relevant links to the fate of microplastics in aquatic continental systems, we here present new insights into the mechanisms of impacts on terrestrial geochemistry, the biophysical environment, and ecotoxicology. Broad changes in continental environments are possible even in particle-rich habitats such as soils. Furthermore, there is a growing body of evidence indicating that microplastics interact with terrestrial organisms that mediate essential ecosystem services and functions, such as soil dwelling invertebrates, terrestrial fungi, and plant-pollinators. Therefore, research is needed to clarify the terrestrial fate and effects of microplastics. We suggest that due to the widespread presence, environmental persistence, and various interactions with continental biota, microplastic pollution might represent an emerging global change threat to terrestrial ecosystems.

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Section: Mechanisms Of Potential Impacts Of Microplastic On Terrestrimentioning

confidence: 99%

Section: Mechanisms Of Potential Impacts Of Microplastic On Terrestrimentioning

confidence: 99%

Section: The Broad‐spectrum Toxicity Targets Of Nanoplasticsmentioning

confidence: 99%

Section: The Broad‐spectrum Toxicity Targets Of Nanoplasticsmentioning

confidence: 99%

See 2 more Smart Citations

Microplastics as an emerging threat to terrestrial ecosystems

Machado

Kloas

Zarfl

et al. 2018

Global Change Biology

1,619

537

View full text Add to dashboard Cite

show abstract

“…NPs is not only dependent on the particle composition itself, but also on the ionic strength in the surrounding medium. Miyazaki et al (2014) found that the NaCl content in the solution has a large impact on the adhesion of polystyrene latex NPs onto the cell surface of S. cerevisiae.…”

Section: Discussionmentioning

confidence: 99%

Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae

Kiefer

Jurisic

Dahlem

et al. 2019

Biotech & Bioengineering

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Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells.However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly(lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/ yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a coculture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency.

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Copper oxide nanoparticles inhibit the metabolic activity of Saccharomyces cerevisiae

Mashock

Kappell

Hallaj

2015

Enviro Toxic and Chemistry

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Copper oxide nanoparticles (CuO NPs) are used increasingly in industrial applications and consumer products and thus may pose risk to human and environmental health. The interaction of CuO NPs with complex media and the impact on cell metabolism when exposed to sublethal concentrations are largely unknown. In the present study, the short-term effects of 2 different sized manufactured CuO NPs on metabolic activity of Saccharomyces cerevisiae were studied. The role of released Cu(2+) during dissolution of NPs in the growth media and the CuO nanostructure were considered. Characterization showed that the 28 nm and 64 nm CuO NPs used in the present study have different primary diameter, similar hydrodynamic diameter, and significantly different concentrations of dissolved Cu(2+) ions in the growth media released from the same initial NP mass. Exposures to CuO NPs or the released Cu(2+) fraction, at doses that do not have impact on cell viability, showed significant inhibition on S. cerevisiae cellular metabolic activity. A greater CuO NP effect on the metabolic activity of S. cerevisiae growth under respiring conditions was observed. Under the tested conditions the observed metabolic inhibition from the NPs was not explained fully by the released Cu ions from the dissolving NPs.

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Adhesion and Internalization of Functionalized Polystyrene Latex Nanoparticles toward the Yeast Saccharomyces cerevisiae

Cited by 5 publications

References 29 publications

Microplastics as an emerging threat to terrestrial ecosystems

Microplastics as an emerging threat to terrestrial ecosystems

Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae

Copper oxide nanoparticles inhibit the metabolic activity of Saccharomyces cerevisiae

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