Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface

Tan, Wee Jin; Teo, Gilbert P.; Liao, Kin; Leong, Kam W.; Mao, Hai‐Quan; Chan, Vincent

doi:10.1016/j.biomaterials.2004.03.041

Cited by 28 publications

(26 citation statements)

References 28 publications

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“…4B). These results were consistent with our previous study [28] on the ability of hydrophobic PEI to adsorbed high amount of FNG and with the data from the literature reporting for high protein adsorption on PET [15]. It is also obvious from figure 4 that the introduction of carboxylic groups on PEI-1 and PEI-2 decreased the quantity of the proteins adsorbed.…”

Section: Protein Adsorptionsupporting

confidence: 82%

“…One approach to improve cell adhesion on biomaterials is surface modification of (hydrophobic) polymers by covalently binding of charged functional groups such as carboxylic (-COOH) groups [15]. It was shown that -COOH moieties on the surface can enhance adsorption of adhesive proteins [such as collagen II and III] as well as to support their fibrilar organization, which subsequently could enhance cell adhesion [FN,16].…”

Section: Introductionmentioning

confidence: 99%

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Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Tzoneva

Seifert

Albrecht

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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Surface modifications of poly(ether imide) (PEI) membranes with carboxylic groups were tested in comparison to pure PEI and poly(ethylene terephtalate) (PET) for their ability to support attachment, growth and function of human umbilical vein endothelial cells (HUVEC) in respect to endothelization of the above materials. Flat sheet PEI membranes were modified by covalent binding of iminodiacetic acid (IDA) for different periods of time (1 to 30 min) to obtain surfaces with various content of carboxylic groups. In addition, fibronectin (FN) and fibrinogen (FNG) preadsorption on the various membranes were studied on their effect on HUVEC behaviour. Results show a decreased protein adsorption and HUVEC adhesion, growth and function in terms of prostacyclin production with an increase carboxylic groups.Preadsorption of the membranes with FN or FNG promoted activity of HUVEC, which became superior to cells on PET. FN-coated membranes were found to be better substrate for HUVEC adhesion and prostacyclin production, while on FNG-coated membranes cells grew better. Overall it can be concluded that PEI is a promising materials for endothelial cells immobilization as it is needed for improving the hemocompatibility of cardiovascular devices.

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Section: Protein Adsorptionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Tzoneva

Seifert

Albrecht

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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“…To further improve the centrifugal seeding process, the effects of scaffold dimension (thickness), porosity, pore size, and surface roughness need to be studied. In addition, incorporation of hydrophilic materials, 1,19 bioactive materials, 24 or peptides 20,25 to the matrix surface to improve the affinity and adhesion of cells to the scaffold surface may also improve cell seeding.…”

Section: Discussionmentioning

confidence: 98%

Centrifugal seeding of mammalian cells in nonwoven fibrous matrices

Gurm

Yang

2009

Biotechnology Progress

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Three-dimensional (3D) cell cultures have many advantages over two-dimensional cultures. However, seeding cells in 3D scaffolds such as nonwoven fibrous polyethylene terephthalate (PET) matrices has been a challenge task in tissue engineering and cell culture bioprocessing. In this study, a centrifugal seeding method was investigated to improve the cell seeding efficiency in PET matrices with two different porosities (93% and 88%). Both the centrifugal force and centrifugation time were found to affect the seeding efficiency. With an appropriate centrifugation speed, a high 80-90% cell seeding efficiency was achieved and the time to reach this high seeding efficiency was less than 5 min. The seeding efficiency was similar for matrices with different porosities, although the optimal seeding time was significantly shorter for the low-porosity scaffold. Post seeding cell viability was demonstrated by culturing colon cancer cells seeded in PET matrices for over 5 days. The centrifugal seeding method developed in this work can be used to efficiently and uniformly seed small fibrous scaffolds for applications in 3D cell-based assays for high-throughput screening.

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“…In particular, the kinetics of cell deadhesion and detachment during the thermal-induced transformation of TRP is not known. Our group has been actively engaged in the development of integrated biophysical techniques to elucidate the adhesion contact dynamics of several cell types on biomaterials during the initial phase of cell seeding [5]. We hypothesize that this biophysical approach may also be beneficial for probing the cell deadhesion/detachment kinetics towards temperature drop on TRP surface.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

Chen¹,

Dang²,

Tan³

et al. 2007

Biomaterials

Self Cite

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Thermoresponsive polymer (TRP) enables the enzyme-free harvesting of cells through an acute increase in surface hydrophilicity of TRP across its lower critical solution temperature (LCST), rendering feasible the generation of polymer-free cell sheets for regenerative medicine applications. To date, the intricate mechanisms of cell deadhesion/detachment on TRP surface remain obscure. Elucidation of such biophysical responses would be valuable for the cell sheet technology. In this study, integrative biophysical techniques are applied to probe the thermal-induced deadhesion kinetics of smooth muscle cell (SMC) on thermoresponsive hydroxybutyl chitosan (HBC29) against different periods of pre-culture time at 37 °C. Atomic force microscopy demonstrates that both the surface topography and mechanical property of HBC29 film in water are acutely modulated across its LCST. Firstly, cells show negligible changes in adhesion contact area during low-temperature incubation on unmodified tissue culture polystyrene (TCPS). Secondly, the recession of adhesion contact and retraction of cell body for cells with different pre-culture times are triggered by HBC29 coating on TCPS. Interestingly, the initial rate of reduction in the normalized adhesion contact area of SMC is negatively correlated with the pre-culture time. Thirdly, the degree of cell deformation and average adhesion energy are reducing functions of time only for SMCs with the lowest preculture time. In contrast, adhesion energy per cell is a reducing function of time irrespective of the change of pre-culture time. Lastly, the temporal dynamics of cytoskeleton organization and β-actin/ smoothelin-B mRNA expression for SMCs is strongly dependent on the pre-culture time. Overall, this study demonstrates that the thermal-induced deadhesion of SMC on TRP is characterized by the evolution of its contractile phenotypes.

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Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface

Cited by 28 publications

References 28 publications

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Centrifugal seeding of mammalian cells in nonwoven fibrous matrices

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

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