Adhesion of Candida tropicalis to polystyrene and epithelial cell lines: Insights of correlation of the extent of adherent yeast cells among distinct surfaces

Souza, Cássia Milena de; Perini, Hugo Felix; Caloni, Carla Custódio; Furlaneto-Maia, Luciana; Furlaneto, Márcia Cristina

doi:10.1016/j.mycmed.2020.101043

Cited by 7 publications

(10 citation statements)

References 25 publications

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“…On the other hand, isolates of C. tropicalis showed adhesion rates to HeLa cells similar to those observed for C. albicans (Okawa et al 2008). When adherence was compared using distinct epithelial cell lines, it was shown that C. tropicalis exhibits cell typedependent differences in adherence rates (Negri et al 2011a;Souza et al 2020a). C. tropicalis was more adherent to intestinal cells (Caco-2) than to cervical cells (HeLa) and urinary cells (TCC-SUP) (Negri et al 2011a).…”

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 73%

“…2A. Souza et al (2020a) showed that the adherence of C. tropicalis to polystyrene was greater than its adherence to epithelial cells (HeLa and Vero cells). These authors found a correlation of adhesion capability between abiotic (polystyrene) and the cell lines.…”

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 99%

“…In another study, isolates of C. tropicalis showed more adherence to HeLa cells than to kidney cells (Vero cells). Furthermore, yeast cells occurred as aggregates on the surface of HeLa cells, while diffuse adhesion was evidenced on Vero cells (Souza et al 2020a). The adhesion patterns of C. tropicalis cells to HeLa and Vero cells are shown in Fig.…”

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 99%

“…This yeast displayed the highest adherence capacity among C. albicans and other Candida nonalbicans pathogenic species. Another feature is that isolates of C. tropicalis obtained from different anatomical sites are capable of adhering to polystyrene in a strain-dependent manner (Silva-Dias et al 2015;Yu et al 2017;Kumari et al 2018;Souza et al 2020a). An illustration of C. tropicalis adhesion on a polystyrene surface is shown in Fig.…”

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 99%

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Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know?

et al. 2023

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Candida tropicalis is among the most important Candida species in terms of epidemiology, virulence and resistance. Considering the increase in C. tropicalis incidence and high rates of mortality associated with this species, knowledge of its adhesion and biofilm formation abilities is needed. These traits determine the persistence and survival of yeast on different indwelling medical devices and host sites. C. tropicalis is among the most adherent Candida species, and it has been described as a strong biofilm producer. Environmental factors, phenotypic switching and quorum sensing molecules can affect adhesion and biofilm growth. C. tropicalis can form sexual biofilms, which are promoted by mating pheromones. C. tropicalis biofilms are regulated by a wide and complex network of genes and signaling pathways that are currently poorly understood. Morphological studies showed improved biofilm architecture, which was related to the expression of several hypha-specific genes. Based on recent updates, research is still needed to increase our knowledge on the genetic network of adhesion and biofilm formation by C. tropicalis, as well as the protein diversity that mediates interactions with inert materials and biological surfaces. Here, we have reviewed the main aspects related to adhesion and biofilm formation in C. tropicalis and summarized current knowledge on the significance of these virulence factors in this opportunistic species.

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Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 73%

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 99%

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 99%

Section: Adhesion Capability Of Candida Tropicalismentioning

confidence: 99%

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Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know?

et al. 2023

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“…The majority of Candida−human cell adhesion studies use standard adhesion assays in which adherent cells are visualized using fluorescence microscopy and quantified by counting the number of adherent yeasts. 15,25,43 More recently, SCFS has been used to quantify adhesion forces between single live yeast and mammalian cells. 44 C. albicans adhesion to skin keratinocyte, gingival fibroblast, and vaginal epithelial cell lines was found to be implicitly biological (Figure 1), consistent with prior studies of other pathogens, with similar adhesion profiles.…”

Section: ■ Discussionmentioning

confidence: 99%

Host Cell Geometry and Cytoskeletal Organization Governs Candida–Host Cell Interactions at the Nanoscale

Ndlovu,

Malpartida,

Sultana

et al. 2023

ACS Appl. Mater. Interfaces

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Candida is one of the most common opportunistic fungal pathogens in humans. Its adhesion to the host cell is required in parasitic states and is important for pathogenesis. Many studies have shown that there is an increased risk of developing candidiasis when normal tissue barriers are weakened or when immune defenses are compromised, for example, during cancer treatment that induces immunosuppression. The mechanical properties of malignant cells, such as adhesiveness and viscoelasticity, which contribute to cellular invasion and migration are different from those of noncancerous cells. To understand host invasion and its relationship with host cell health, we probed the interaction of Candida spp. with cancerous and noncancerous human cell lines using atomic force microscopy in the single-cell force spectroscopy mode. There was significant adhesion between Candida and human cells, with more adhesion to cancerous versus noncancerous cell lines. This increase in adhesion is related to the mechanobiological properties of cancer cells, which have a disorganized cytoskeleton and lower rigidity. Altered geometry and cytoskeletal disruption of the human cells impacted adhesion parameters, underscoring the role of cytoskeletal organization in Candida–human cell adhesion and implicating the manipulation of cell properties as a potential future therapeutic strategy.

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One-Pot and Environmentally Friendly Biosynthesis of Silver Nanoparticles from Enterococcus durans: Activity Against Fluconazole-Resistant Pathogenic Candida tropicalis

Paulo,

de Souza,

de Souza

et al. 2024

BioNanoSci.

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Adhesion of Candida tropicalis to polystyrene and epithelial cell lines: Insights of correlation of the extent of adherent yeast cells among distinct surfaces

Cited by 7 publications

References 25 publications

Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know?

Adhesion and biofilm formation by the opportunistic pathogen Candida tropicalis: what do we know?

Host Cell Geometry and Cytoskeletal Organization Governs Candida–Host Cell Interactions at the Nanoscale

One-Pot and Environmentally Friendly Biosynthesis of Silver Nanoparticles from Enterococcus durans: Activity Against Fluconazole-Resistant Pathogenic Candida tropicalis

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