Adhesion of Human Osteoblasts on Root-End Filling Materials

Abstract

Adhesion of human osteoblasts to root-end filling materials (mineral trioxide aggregate (MTA), IRM, composite, and amalgam) was observed by scanning electron microscopy. Root-end filling materials were inserted into 96-well flat-bottomed plates and condensed to disks of approximately 1 mm thick and the same diameter as the wells. After the disks were set, they were placed in the bottom of Nunc four-well culture plates at one disk per well. Then human osteoblasts were seeded into the wells at 1.5 x 10(5) cells per well. After 1 day in culture the disks of root-end filling materials along with cells grown on their surface were examined with a scanning electron microscopy. Results showed that osteoblasts attached and spread on MTA and composite by forming a monolayer. Osteoblasts also attached on amalgam, but with few cells spreading. In the presence of IRM, osteoblasts appeared rounded with no spreading. These results indicate that osteoblasts have a favorable response to MTA and composite resin compared with IRM and amalgam.

Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex

“…Use of human primary cells of a relevant type to the study of endodontic materials, has been pointed out previously (12), in view of several expected differences in the responses of immortalized cells. For this reason, hOB cells have been considered closest to the ideal cells for cytocompatibility assays, because the direct interaction of these cells with biomaterials could play a critical role in a clinical setting (13). It was also reported that hOB cells provide a useful tool able to help predicting the effects of biomaterials on regenerative capability of periapical tissues (14).…”

Chemical and morphological characteristics of mineral trioxide aggregate and Portland cements

“…2 and 3). The fine particle size and distribution of WMTA is generally considered clinically advantageous in comparison to PC, because physical structure, surface characteristics and cytotoxicity of a material indicates its biocompatibility 30) . Nonetheless, dental materials with smoother surfaces causes less irritation in adjacent living tissues 31) .…”

In VitroComparison of the Effects of Dental Filling Materials on Mouse Fibroblasts

“…Adhesion and spreading of cells on a material surface are initial phase for cellular function. Zhu et al (62) suggested that cell adhesion and spreading on restorative materials could be used as a criterion for evaluation of the biocompability of that material. In studies about the effects of various restorative materials on human PDL cells (3,4), it was proposed that the observation of circular shaped fibroblasts are due to the toxic effects of the surface materials.…”