2024
DOI: 10.1073/pnas.2411617121
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Adhesive interactions within microbial consortia can be differentiated at the single-cell level through expansion microscopy

Pu-Ting Dong,
Wenyuan Shi,
Xuesong He
et al.

Abstract: Investigating microbe–microbe interactions at the single-cell level is critical to unraveling the ecology and dynamics of microbial communities. In many situations, microbes assemble themselves into densely packed multispecies biofilms. The density and complexity pose acute difficulties for visualizing individual cells and analyzing their interactions. Here, we address this problem through an unconventional application of expansion microscopy, which allows for the “decrowding” of individual bacterial cells wit… Show more

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