Adipocyte Biology from the Perspective of In Vivo Research: Review of Key Transcription Factors

Evseeva, Maria N.; Balashova, Maria S.; Kulebyakin, Konstantin; Rubtsov, Yuri P.

doi:10.3390/ijms23010322

Cited by 11 publications

(5 citation statements)

References 132 publications

(160 reference statements)

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“…Therefore, as observed after ISO treatment, ANGPTL3-Fld-treated cells also displayed increased phosphorylation of several kinases involved in the energy metabolism [17][18][19]. In particular, the signal transduction of ANGPTL3-Fldtreated cells starts with the activation of eNOS, a well-known target of AMPK in the energy metabolism [17][18][19]. Finally, the cotreatment activated the smallest number of kinases such as the YES kinase, RSK1 in the serine 380, and STAT3 in the Y727 [17].…”

Section: Angptl3-fld Activates the Pdgfrβ Receptormentioning

confidence: 77%

“…Conversely, the treatment with ANGPTL3-Fld activates mainly the BRAF-ERK arm of the MAPK pathway (with the activation of CREB and PYK2, the endothelial nitric oxide synthase (eNOS), the STAT3 and STAT6, and the Heat Shock Proteins (HSP60 and HSP27). Therefore, as observed after ISO treatment, ANGPTL3-Fld-treated cells also displayed increased phosphorylation of several kinases involved in the energy metabolism [17][18][19]. In particular, the signal transduction of ANGPTL3-Fldtreated cells starts with the activation of eNOS, a well-known target of AMPK in the energy metabolism [17][18][19].…”

Section: Angptl3-fld Activates the Pdgfrβ Receptormentioning

confidence: 79%

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The Fibrinogen-like Domain of ANGPTL3 Facilitates Lipolysis in 3T3-L1 Cells by Activating the Intracellular Erk Pathway

et al. 2022

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Background: ANGPTL3 stimulates lipolysis in adipocytes, but the underlying molecular mechanism is yet unknown. The C-terminal fibrinogen-like domain of ANGPTL3 (ANGPTL3-Fld) activates the AKT pathway in endothelial cells. Hence, we evaluated whether ANGPTL3-Fld stimulates lipolysis in adipocytes through the MAPK kinase pathway. Materials and Methods: 3T3-L1 adipocytes were treated with isoproterenol (ISO), ANGPTL3-Fld, or both. Lipolysis was evaluated through the release of free fatty acids (FFAs) in the culture medium. The activation status of intracellular kinases was evaluated with and without the inhibition of the BRAF–ERK arm of the MAPK pathway. Results: ANGPTL3-Fld alone was not able to activate lipolysis, while the combination of ANGPTL3-Fld and ISO determined a 10-fold enrichment of the FFA concentration in the culture medium with an incremental effect (twofold) when compared with ISO alone. ANGPTL3-Fld alone inhibited hormone-sensitive lipase (HSL), whereas the treatment with ISO induced the activation of HSL. The net balance of ANGPTL3-Fld and ISO cotreatment resulted in HSL activation. The results indicate that ANGPTL3-Fld generated an intracellular activation signal involving the MAPK–ERK pathway, possibly through the PDGFRβ—PLCγ-AMPK axis. Conclusion: ANGPTL3-Fld appears to act as a facilitator of lipolysis in adipocytes, and this effect was driven by a signal mediated by a pathway that is different from the canonical β-adrenergic stimulus.

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Section: Angptl3-fld Activates the Pdgfrβ Receptormentioning

confidence: 77%

Section: Angptl3-fld Activates the Pdgfrβ Receptormentioning

confidence: 79%

The Fibrinogen-like Domain of ANGPTL3 Facilitates Lipolysis in 3T3-L1 Cells by Activating the Intracellular Erk Pathway

et al. 2022

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“…CEBPB and CEBPA (CCAAT/enhancer-binding protein-beta and alpha) are known TFs in the adipogenic differentiation cascade. Previous studies have elucidated their roles in AT’s developmental stage-specific transcriptional networks [ 38 ]. Several hub genes, such as TOP2A, CENPF, TYMS, AURKA, KIF4A, and KIF20A, are related to the cell cycle and DNA replication, implicating the close relationship between obesity and cancer [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Visceral Adipose Tissue Molecular Networks and Regulatory microRNA in Pediatric Obesity: An In Silico Approach

Roy

Modi

Ghosh

et al. 2022

IJMS

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Childhood obesity carries an increased risk of metabolic complications, sleep disturbances, and cancer. Visceral adiposity is independently associated with inflammation and insulin resistance in obese children. However, the underlying pathogenic mechanisms are still unclear. We aimed to detect the gene expression pattern and its regulatory network in the visceral adipose tissue of obese pediatric individuals. Using differentially-expressed genes (DEGs) identified from two publicly available datasets, GSE9624 and GSE88837, we performed functional enrichment, protein–protein interaction, and network analyses to identify pathways, targeting transcription factors (TFs), microRNA (miRNA), and regulatory networks. There were 184 overlapping DEGs with six significant clusters and 19 candidate hub genes. Furthermore, 24 TFs targeted these hub genes. The genes were regulated by miR-16-5p, miR-124-3p, miR-103a-3p, and miR-107, the top miRNA, according to a maximum number of miRNA–mRNA interaction pairs. The miRNA were significantly enriched in several pathways, including lipid metabolism, immune response, vascular inflammation, and brain development, and were associated with prediabetes, diabetic nephropathy, depression, solid tumors, and multiple sclerosis. The genes and miRNA detected in this study involve pathways and diseases related to obesity and obesity-associated complications. The results emphasize the importance of the TGF-β signaling pathway and its regulatory molecules, the immune system, and the adipocytic apoptotic pathway in pediatric obesity. The networks associated with this condition and the molecular mechanisms through which the potential regulators contribute to pathogenesis are open to investigation.

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“…Therefore, IGF1, glucocorticoid, and cAMP as differentiation inducers could trigger preadipocytes entering into a series of differentiation programs, including mitotic clonal expansion (MCE) and differentiation cascade [ 7 ]. Numerous adipocyte-specific transcription factors were involved in transcriptional differentiation cascade, including cAMP response element-binding protein (CREB), CCAAT enhancer-binding protein α, β and δ (C/EBPα, C/EBPβ, and C/EBPδ), and peroxisome proliferator-activated receptor gamma (PPARγ) [ 20 ]. The phosphorylation of CREB is thought to be the initiation to these events, and active CREB can promote the expression of endogenous C/EBPβ and C/EBPδ [ 21 , 22 ].…”

Section: Process Of Adipogenesismentioning

confidence: 99%

Non-Coding RNAs and Adipogenesis

Zhang

Liu

et al. 2023

IJMS

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Adipogenesis is regarded as an intricate network in which multiple transcription factors and signal pathways are involved. Recently, big efforts have focused on understanding the epigenetic mechanisms and their involvement in the regulation of adipocyte development. Multiple studies investigating the regulatory role of non-coding RNAs (ncRNAs) in adipogenesis have been reported so far, especially lncRNA, miRNA, and circRNA. They regulate gene expression at multiple levels through interactions with proteins, DNA, and RNA. Exploring the mechanism of adipogenesis and developments in the field of non-coding RNA may provide a new insight to identify therapeutic targets for obesity and related diseases. Therefore, this article outlines the process of adipogenesis, and discusses updated roles and mechanisms of ncRNAs in the development of adipocytes.

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Adipocyte Biology from the Perspective of In Vivo Research: Review of Key Transcription Factors

Cited by 11 publications

References 132 publications

The Fibrinogen-like Domain of ANGPTL3 Facilitates Lipolysis in 3T3-L1 Cells by Activating the Intracellular Erk Pathway

The Fibrinogen-like Domain of ANGPTL3 Facilitates Lipolysis in 3T3-L1 Cells by Activating the Intracellular Erk Pathway

Visceral Adipose Tissue Molecular Networks and Regulatory microRNA in Pediatric Obesity: An In Silico Approach

Non-Coding RNAs and Adipogenesis

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