Adipocyte telomere length associates negatively with adipocyte size, whereas adipose tissue telomere length associates negatively with the extent of fibrosis in severely obese women

Bouazzaoui, Fatiha el; Henneman, Peter; Thijssen, Peter; Visser, Annemieke; Koning, Frits; Lips, Mirjam A.; Janssen, Ignace; Pijl, Hanno; Dijk, Ko Willems van; Harmelen, Vanessa van

doi:10.1038/ijo.2013.175

Cited by 22 publications

(15 citation statements)

References 12 publications

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“…The relative length of telomeres was estimated by quantitative PCR as previously reported [40, 41]. Briefly, genomic DNA was extracted with Qiagen DNeasy Blood & Tissue Kit (Qiagen, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Mitochondrial DNA Hypomethylation Is a Biomarker Associated with Induced Senescence in Human Fetal Heart Mesenchymal Stem Cells

Pian

et al. 2017

Stem Cells International

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Background. Fetal heart can regenerate to restore its normal anatomy and function in response to injury, but this regenerative capacity is lost within the first week of postnatal life. Although the specific molecular mechanisms remain to be defined, it is presumed that aging of cardiac stem or progenitor cells may contribute to the loss of regenerative potential. Methods. To study this aging-related dysfunction, we cultured mesenchymal stem cells (MSCs) from human fetal heart tissues. Senescence was induced by exposing cells to chronic oxidative stress/low serum. Mitochondrial DNA methylation was examined during the period of senescence. Results. Senescent MSCs exhibited flattened and enlarged morphology and were positive for the senescence-associated beta-galactosidase (SA-β-Gal). By scanning the entire mitochondrial genome, we found that four CpG islands were hypomethylated in close association with senescence in MSCs. The mitochondrial COX1 gene, which encodes the main subunit of the cytochrome c oxidase complex and contains the differentially methylated CpG island 4, was upregulated in MSCs in parallel with the onset of senescence. Knockdown of DNA methyltransferases (DNMT1, DNMT3a, and DNMT3B) also upregulated COX1 expression and induced cellular senescence in MSCs. Conclusions. This study demonstrates that mitochondrial CpG hypomethylation may serve as a critical biomarker associated with cellular senescence induced by chronic oxidative stress.

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Section: Methodsmentioning

confidence: 99%

“…The telomere length was estimated by the relative ratio between the copies of telomere and the copies of β -globin (T/S). T/S value is calculated by 2 −ΔΔCt [40, 41]. …”

Section: Methodsmentioning

confidence: 99%

Mitochondrial DNA Hypomethylation Is a Biomarker Associated with Induced Senescence in Human Fetal Heart Mesenchymal Stem Cells

Pian

et al. 2017

Stem Cells International

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“…We observed only a few crown-like structures in obese and more specifically in SC adipose tissue 116 . No correlation was found between the number of crown-like structures and adipocyte hypertrophy in humans 65,74,76,117 .…”

Section: Inflammationmentioning

confidence: 97%

Adipocyte size as a determinant of metabolic disease and adipose tissue dysfunction

Laforest¹,

Labrecque²,

Michaud³

et al. 2015

Critical Reviews in Clinical Laboratory Sciences

186

148

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Obesity is a heterogeneous disease and is associated with comorbidities such as type 2 diabetes mellitus, cardiovascular disease and cancer. Several studies have examined the role of dysfunctional adipose tissue in the pathogenesis of obesity, highlighting the contrasting properties and impact of distinct fat compartments, sometimes with contradictory results. Dysfunctional adipose tissue involves enlargement, or hypertrophy, of pre-existing fat cells, which is thought to confer increases in cardiometabolic risk, independent of the level of obesity per se. In this article, we critically analyze available literature that examined the ability of adipocyte cell size to predict metabolic disease and adipose tissue dysfunction in humans. Many studies demonstrate that increased fat cell size is a significant predictor of altered blood lipid profiles and glucose-insulin homeostasis independent of adiposity indices. The contribution of visceral adiposity to these associations appears to be of particular importance. However, available studies are not unanimous and many fat depot-specific aspects of the relationship between increased fat cell size and cardiometabolic risk or parameters of adipose tissue dysfunction are still unresolved. Methodological factors such as the approach used to express the data may represent significant confounders in these studies. Additional studies should consider the fact that the relationship between fat cell size and common adiposity indices is non-linear, particularly when reaching the obese range. In conclusion, our analysis demonstrates that fat cell size is a significant predictor of the cardiometabolic alterations related to obesity. We propose that adipocyte hypertrophy, especially in the visceral fat compartment, may represent a strong marker of limited hyperplasic capacity in subcutaneous adipose tissues, which in turn is associated with the presence of numerous cardiometabolic alterations.

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“…Bouazzaoui et al [25] reported that there was no correlation between the telomere length of obese human visceral adipose tissue and visceral adipocyte size. Lakowa et al [8] indicated that, in humans, there is no difference in telomere length between mature subcutaneous and mature visceral adipocytes.…”

Section: Discussionmentioning

confidence: 98%

Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues

Yamada

Higuchi

Nakanishi

2015

Biochemical and Biophysical Research Communications

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Adipocyte telomere length associates negatively with adipocyte size, whereas adipose tissue telomere length associates negatively with the extent of fibrosis in severely obese women

Cited by 22 publications

References 12 publications

Mitochondrial DNA Hypomethylation Is a Biomarker Associated with Induced Senescence in Human Fetal Heart Mesenchymal Stem Cells

Mitochondrial DNA Hypomethylation Is a Biomarker Associated with Induced Senescence in Human Fetal Heart Mesenchymal Stem Cells

Adipocyte size as a determinant of metabolic disease and adipose tissue dysfunction

Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues

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