Adiponectin and Its Effects on Acute Leukemia Cells: An Experimental and Bioinformatics Approach

Tsartsalis, Athanasios N.; Tagka, Anna; Κωτούλας, Αθανάσιος; Mirkopoulou, Daphne; Geronikolou, Styliani; Lambrou, George Ι.

doi:10.1007/978-3-030-78775-2_14

Cited by 1 publication

(2 citation statements)

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“…To our knowledge, only one study has focused on exploring the activity of adiponectin on lymphoblastic cells [ 21 ]; therefore, the aim of the present work was to investigate the role of adiponectin and its effects on a B lymphoblastic cell line, the JVM-2 cells, taken as a disease model. The cells were treated with different concentrations of AdipoRon, an adiponectin agonist, for up to 72 h to evaluate cytotoxicity, apoptosis, autophagy and cell cycle distribution.…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, such an effect has never been reported before and might be peculiar to adiponectin’s action on lymphoblast cells. Indeed, only one study analyzed the in vitro effects of adiponectin on lymphoblastic cells, reporting that adiponectin did not produce any inhibition of cell cycle progression, as well as an increase in cell death while regulating different genes, such as CD22, CDH1, IFNG, LCK, MSH2, SPINT2, implicated in the progression of leukemia [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Adiponectin on the Behavior of B-Cell Leukemia Cells: Insights from an In Vitro Study

Mallardo,

Scalia,

Raia

et al. 2023

Biomedicines

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Background: Non-Hodgkin’s lymphoma (NHL), the most frequent hematological neoplasm worldwide, represents a heterogeneous group of malignancies. The etiology of NHL remains to be fully elucidated, but the role of adipose tissue (AT) in immune function via the secretion of adipokines was recently recognized. Among adipokines, adiponectin has garnered attention for its beneficial properties. This study aimed to explore the in vitro effects of AdipoRon, an adiponectin agonist, on JVM-2, a lymphoblast cell line used as a representative disease model. Methods: JVM-2 cells were treated with different concentrations of AdipoRon to evaluate its effects on viability (via an MTT test), cell cycle distribution (via an FACS analysis), invasiveness (via a Matrigel assay) and colony-forming ability; protein expression was assessed via a real-time PCR (qPCR) and/or Western blotting (WB). Results: We found that the prolonged exposure of JVM-2 cells to AdipoRon led to a reduction in their viability due to a cytostatic effect. Additionally, AdipoRon stimulated both the formation of cell colonies and the expression of E-cadherin. Interestingly, the administration of AdipoRon increased the invasive potential of JVM-2 cells. Conclusions: Our findings indicate that adiponectin is involved in the regulation of different cellular processes of JVM-2 cells, supporting its potential association with a pro-tumorigenic phenotype and indicating that it might contribute to the increased aggressiveness and metastatic potential of B lymphoma cells. However, additional studies are required to fully understand the molecular mechanisms of adiponectin’s actions on lymphoblasts and whether it may represent a marker of disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%