Adiponectin and Testosterone Levels and Their Correlations with Fertility in Anglo-Nubian x Etawah Grade Crossbred Bucks

Hafizuddin, Hafizuddin; Karja, Ni Wayan Kurniani; Praharani, Lisa; Setiadi, Mohamad Agus

doi:10.5398/tasj.2020.43.2.110

Cited by 9 publications

(14 citation statements)

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“…This assay has been successfully validated for measuring adiponectin concentrations in Anpera buck blood plasma. Adiponectin measurement was performed by manufacturer's instructions, according to Hafizuddin et al (2020). Briefly, 50 μl adiponectin standard were added to standard wells and 40 μL of blood plasma sample) were placed in the sample wells.…”

Section: Adiponectin Concentration Measurementsmentioning

confidence: 99%

“…This study finds breed is a factor in increasing adiponectin, as shown in the Anglo Nubian breed. Specific factors in goat breed are likely to produce differences in the concentration of adiponectin (Hafizuddin et al 2020). However, the identification of factors influencing adiponectin concentration, especially in goats, requires further research.…”

Section: The Concentration Of Adiponectinmentioning

confidence: 99%

“…Recent studies by Hafizuddin et al (2020) show that the hormone adiponectin correlates with buck semen characteristics, starting with variances in semen volume, sperm morphology, and sperm concentration. This corroborates previous reports on male mammals (Kasimanickam et al 2013;Thomas et al 2013;Kadivar et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

et al. 2021

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Abstract. Hafizuddin, Karja NWK, Praharani L, Setiadi MA. 2021. Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks. Biodiversitas 22: 1112-1119. The purpose of this study was to evaluate the effect of differences in breed and age on reproductive performance and adiponectin and testosterone production in Anglo Nubian, Etawah grade, and crossbred (Anpera) bucks. A total of 12 bucks with four individuals of each breed were used. This study collected five data points from each buck regarding adiponectin, testosterone, and reproductive performance (libido and semen characteristics). Data were analyzed with factorial analysis of variance and Duncan's test. The result shows that adiponectin concentration between breeds was significantly different (P <0.01). There were also significant differences (P<0.05) in adiponectin concentrations based on buck age. There were also significant interactions with adiponectin concentrations (P <0.01). Furthermore, testosterone concentrations showed significant differences based on breed (P <0.05) and age (P <0.05). There were also significant age-breed interactions affecting testosterone concentrations (P <0.01). Libido and semen characteristics had no significant differences based on breed and age group, and no significant age-breed interactions (P> 0.05). The heterosis effect on adiponectin concentration (48.05%), testosterone concentration (27.68%), libido (-0.61%), semen volume (-1.93%), sperm motility (0.49%), sperm normal morphology (0.18%), and sperm concentration (0.00%) was measured. In conclusion, there is a significant effect of breed, age, and age-breed interactions on the concentration of adiponectin and testosterone in bucks, and both of these variables have a high heterosis effect on crossbred bucks.

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Section: Adiponectin Concentration Measurementsmentioning

confidence: 99%

Section: The Concentration Of Adiponectinmentioning

confidence: 99%

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Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

et al. 2021

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“…According to Felton-Taylor et al [5], sperm abnormalities reduced the fertilization rate and the ability of sperm to penetrate the zona pellucida. These abnormalities increased in older bulls, possibly due to either low testosterone hormone concentration [6] or low adiponectin concentration [7]. Due to this condition, seminiferous germinal tubules decreased, which play an essential role in spermatogenesis [3].…”

Section: Introductionmentioning

confidence: 99%

“…Due to this condition, seminiferous germinal tubules decreased, which play an essential role in spermatogenesis [3]. Hafizuddin et al [7] stated that testosterone concentration, including sperm abnormalities, was not correlated with age. However, either high or low concentrations of testosterone can affect the morphological formation (normal or abnormal) of sperm [8] through the hypothalamus-pituitary-gonad axis mechanism, which is important for spermatogenesis [9].…”

Section: Introductionmentioning

confidence: 99%

Correlation between age, testosterone and adiponectin concentrations, and sperm abnormalities in Simmental bulls

et al. 2021

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Background and Aim: Capacity for sperm production is affected by age, which is related to the morphology of sperm abnormalities and can affect fertility. The aim of this study was to evaluate the relationship between age and concentrations of testosterone and adiponectin with sperm abnormalities in Simmental bulls. Materials and Methods: The study used 11 bulls, separated into three groups. The first group consisted of five bulls aged 4-5 years, and the second and third groups each consisted of three bulls, aged 6-7 and 8-10 years, respectively. The average sperm motility of the animals ranged from 57.66±2.60% to 70.17±0.22%. Blood samples were obtained from the coccygeal region of the animals. Testosterone and adiponectin analysis was performed using the enzyme-linked immunosorbent assay method. Sperm morphology was evaluated using carbol fuchsin-eosin staining according to the Williams method. Finally, correlations between testosterone and adiponectin concentrations, age, and sperm abnormalities were analyzed using Pearson's correlation analysis. Results: The findings revealed a significant correlation (p<0.01) between the concentrations of testosterone and adiponectin (–0.538), age (–0.588), and abnormal sperm morphology (–0.912). Moreover, they revealed that the concentration of testosterone in the bulls aged 8-10 years was lower, at 21.89±4.56 ng/mL, compared to that in the bulls aged 4-5 years, at 36.15±1.29 ng/mL, and 6-7 years, at 35.16±5.39 ng/mL. The findings also revealed a positive correlation between adiponectin concentration and age (0.529) and sperm abnormalities (0.506). The increase in testosterone concentration was inversely related to the adiponectin concentration (–0.538). Moreover, the mean amount of abnormal sperm increased with increasing age: 3.82±0.33% in the group aged 4-5 years, and 4.40±0.72% and 10.20±1.97% in the groups aged 6-7 years and 8-10 years, respectively. Conclusion: The study data indicate that there is a decrease in testosterone concentration, a high adiponectin concentration, and an increase in abnormal sperm with increasing age in bulls.

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Association of Gonadotropin-Releasing Hormone Receptor (GnRHR) gene (c.18085 T>G) and litter size in Ettawa crossbreed goat

Hidayah,

Ardiantoro,

Septian

et al. 2023

BIO Web of Conferences

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In the present study, the association of Gonadotropin-Releasing Hormone Receptor gene (GnRHR) and litter size were investigated using the PCR-RFLP method as a genetic marker candidate for litter size in 35 individuals from Ettawa crossbreed goats. This research has two steps conducted in the laboratory and in the field. Data on litter size and blood samples were collected from the field. In the laboratory was carried out DNA isolation, amplification, and restriction. Molecular analysis of DNA isolation were precise, the enzyme Msp1 (C*CGG) with a product length of 706 bp was used for amplification and restriction analysis, releaved TT genotype in GnRHR gene fragment. In the exon 3 of chromosome 6, the single nucleotide polymorphism (SNP) c.18085 T>G was found. Ettawa crossbreed goat in calculations using the hardy-weinberg law, insignificant results are obtained because the value is more than > 0.05 (db; 1) = 0.50. The genotypes and allele frequencies found in the Ettawa Crossbreed Goat GnRHR genes are monomorphic and the GnRHR gene did not associate with litter size. The conclusion is that GnRHR exon 3 gene (SNP) c.18085 T>G did not associate with litter size and can not be used as a genetic marker on Ettawa crossbred goats.

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Adiponectin and Testosterone Levels and Their Correlations with Fertility in Anglo-Nubian x Etawah Grade Crossbred Bucks

Cited by 9 publications

References 0 publications

Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

Correlation between age, testosterone and adiponectin concentrations, and sperm abnormalities in Simmental bulls

Association of Gonadotropin-Releasing Hormone Receptor (GnRHR) gene (c.18085 T>G) and litter size in Ettawa crossbreed goat

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