Adiponectin enhances in vitro development of swine embryos

Chappaz, Eugénie; Albornoz, Marcelo S.; Campos, Danila Barreiro; Che, Limei; Palin, Marie‐France; Murphy, Bruce D.; Bordignon, Vilceu

doi:10.1016/j.domaniend.2008.05.007

Cited by 73 publications

(66 citation statements)

References 34 publications

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“…Adiponectin addition to the medium induced meiotic maturation of oocytes derived from large follicles, but not those derived from small follicles (Chappaz et al 2008). The growth and regression of ovarian tissues are accompanied by equally rapid changes in their vascular vessel network.…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

Szeszko

Smolińska

Kieżun

et al. 2015

Funct Integr Genomics

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Reproductive functions are closely related to nutritional status. Recent studies suggest that adiponectin may be a hormonal link between them. Adiponectin is an adipocytokine, abundantly expressed in adipose tissues. It plays a dominant role in lipid and carbohydrate metabolism by stimulating fatty acid oxidation, decreasing plasma triglycerides, and increasing cells' sensitivity to insulin and has direct antiatherosclerotic effects. The hormone is also postulated to play a modulatory role in the regulation of the reproductive system. The aim of this study was to identify differentially expressed genes (DE-genes) in response to adiponectin treatment of porcine luteal ovarian cells. The global expression of genes in the porcine ovary was investigated using the Porcine (V2) Two-color gene expression microarray, 4 × 44 (Agilent, USA). Analysis of the microarray data showed that 701 genes were differentially expressed and 389 genes showed a fold change greater than 1.2 (p < 0.05). Among this number, 186 genes were up-regulated and 203 were down-regulated. The list of DE-genes was used for gene ontology analyses. The biological process list was generated from up-regulated and down-regulated DE-genes. We found that up-regulated products of DE-genes take part in 30 biological processes and down-regulated products in 9. Analysis of the interaction network among DE-genes showed that adiponectin interacts with genes involved in important processes in luteal cells. These results provide a basis for future work describing the detailed interactions and relationships explaining local regulation of adiponectin actions in the ovary of pigs.

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Section: Discussionmentioning

confidence: 95%

“…Knowledge of the effects of adiponectin on the development of the ovaries is limited mainly to oocytes' maturation and vascularization (Chappaz et al 2008;Ledoux et al 2006). Adiponectin addition to the medium induced meiotic maturation of oocytes derived from large follicles, but not those derived from small follicles (Chappaz et al 2008).…”

Section: Discussionmentioning

confidence: 99%

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

Szeszko

Smolińska

Kieżun

et al. 2015

Funct Integr Genomics

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“…Also, rh adiponectin alone did not stimulate mouse COCs expansion; however, it enhanced significantly the in vitro fertilization and development of the mouse embryos to the blastocyst stage [7]. In the pig, however, Chappaz et al [13] found that recombinant porcine adiponectin significantly decreased the frequency of meiotic immature oocytes derived from large follicles (3-6 mm) but not from small follicles (< 3 mm), suggesting an acceleration of the meiotic maturation of porcine oocyte by adiponectin. This discrepancy between different studies could be linked to the animal species or to the size of the follicles used for COCs recovery and also the dose of the adiponectin they used.…”

Section: Discussionmentioning

confidence: 97%

“…For example, in chickens and pigs, there were no variations in the levels of adiponectin receptors in granulosa cells compared to theca cells or among different sizes of follicles [10][11][12]. It has also been shown that adiponectin differentially alters the expression of genes involved in steroidogenesis, ovulation and apoptosis in cumulus cells and enhances oocyte maturation and early embryo development in the mouse, human and the swine [6,13]. No significant effects were observed on the bovine in vitro oocyte maturation, cleavage and blastocyst formation rates in the presence of adiponectin [14].…”

Section: Introductionmentioning

confidence: 99%

The In Vitro Effect of Adiponectin on Early Bovine Embryonic Development and Transcriptomic Markers of Oocyte Competence

MR¹,

Kafi²

2016

JFIV Reprod Med Genet

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Objectives: The current study aimed to investigate the in vitro effects of physiologic concentration of adiponectin in follicular fluid of pre-ovulatory bovine ovarian follicles on: 1) the bovine oocyte maturation and the early developmental rates of bovine embryos in vitro; 2) the expression profiles of some developmentally important marker genes in the bovine cumulus cells and oocytes. Materials and Methods:The cumulus-oocyte complexes (COCs) were aspirated from abattoir ovaries and cultured in vitro in the presence/absence of the recombinant human (rh) adiponectin (5 µg/mL) and used for real time RT-QPCR and in vitro embryo production assays.Results: Adiponection had no significant effects on nuclear maturation and cumulus expansion rates (p > 0.05). The expression levels of hyaluronic synthase 2 gene (Has2) was significantly higher in the adiponection group compared to the control group (p = 0.014). However, the early embryonic development was not affected significantly in the presence of adiponectin. Adiponectin did not change the expression rates of adiponectin receptors or other markers of oocyte competence. Also, the expression rates of two important oocyte secreted factors (OSFs), bone morphogenetic protein 15 (Bmp15) and growth/differentiation factor 9 (Gdf9) were not affected (p > 0.05). Conclusions:Adiponection did not affect the bovine oocyte developmental competence and early embryo development in spite of a significant increase in the expression level of Has2 gene, a biomarker of the future oocyte developmental competence.

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“…Dans les cellules de granulosa de porc cultivées in vitro, 1'adiponectine augmente 1'expression de molécules impliquées dans 1'ovulation, telles que Cox2 (cyclooxygénase 2), PGE2 (prostaglandine E2) et EGF (epidermal growth factor) [22]. Lors de protocoles de fécondation in vitro chez la femme, la souris et le porc, 1'adiponectine améliore la maturation ovocytaire et le développement de l'embryon [29,30].…”

Section: Rôle De L'adiponectine Et De La Résistine Au Niveau Des Gonadesunclassified

Adiponectine et résistine

et al. 2013

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Adiponectin enhances in vitro development of swine embryos

Cited by 73 publications

References 34 publications

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

The In Vitro Effect of Adiponectin on Early Bovine Embryonic Development and Transcriptomic Markers of Oocyte Competence

Adiponectine et résistine

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